LOCUS       sequence-225662-       10825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, 
            Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH, Spector DL
  TITLE     From silencing to gene expression: real-time analysis in single 
            cells.
  JOURNAL   Cell 2004 Mar 5;116(5):683-98.
  PUBMED    15006351
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2004-03-5"; volume: "116(5)"; pages: "683-98"
COMMENT     Sequence Label: CMV-H3.3-YFP
FEATURES             Location/Qualifiers
     source          1..10825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2762..2792,2793..2994)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3056..3150
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3169..3407
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3354..3375
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     enhancer        3505..3884
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3885..4088
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4039..4059
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4133..4152
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4133..4151
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4629..4638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4635..4637,4638..4640,4641..5351)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4680..4701)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4941..4960)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5288..5309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          5471..5536
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5666..5686
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5958..6092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5995..6014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6049..6068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6119..6137)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6589..6660,7062..7335,7410..8064,8126..8441,8645..8776,
                     8847..9461)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(10240..10794,10795..10825)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     2701 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     2761 ccatgatgaa ataacataag gtggtcccgt cggcaagaga catccactta acgtatgctt
     2821 gcaataagtg cgagtgaaag gaatagtatt ctgagtgtcg tattgagtct gagtgagaca
     2881 gcgatatgat tgttgattaa cccttagcat gtccgtgggg tttgaattaa ctcataatat
     2941 taattagacg aaattatttt taaagtttta tttttaataa tttgcgagta cgcaaagctt
     3001 ggctgcatcc aacgcgttgg gagctctccg gatccaagct tgcatgcctg caggtcggag
     3061 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     3121 tgtcctccga gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa
     3181 tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc
     3241 taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa
     3301 gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta
     3361 ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg
     3421 gaattccaaa acaaactggt tattgtggta ggtcatttgt ttggcagaaa gaaaactcga
     3481 gtgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     3541 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3601 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3661 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3721 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3781 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3841 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     3901 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     3961 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     4021 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     4081 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     4141 ctcactatag ggagacccaa gcttggacct ctagagctca aaaccactta attaccaggt
     4201 aaacgatggc acgtaccaag caaacagccc gtaaatcgac cggaggcaag gcgccccgca
     4261 agcagctggc caccaaggcg gcccgtaaat cggcgccatc caccggcgga gtgaagaagc
     4321 cacatcgcta ccgtcctgga acggtggccc tgcgtgagat tcgtcgctac cagaagtcca
     4381 cggagctgct catccgcaag ctgccgttcc agcgtctggt gcgcgagata gcccaggact
     4441 tcaagaccga tctgcgcttc cagtcggcgg ccattggagc cctacaggag gccagcgagg
     4501 cgtacctggt cggtctgttc gaggacacca atctgtgcgc cattcacgcc aagcgcgtca
     4561 ccattatgcc caaggacatc cagctggcca gacgcatccg tggcgagcgg gcctcacggc
     4621 cgtcggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4681 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     4741 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4801 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc
     4861 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4921 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4981 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5041 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5101 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     5161 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5221 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     5281 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5341 agctgtacaa gtcacggcca gtcgcaacaa gatctgatta aggtaccact agaggatctt
     5401 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     5461 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     5521 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     5581 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     5641 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     5701 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     5761 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     5821 aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     5881 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5941 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6061 aactcatcaa tgtatcttat catgtctgga tccactagaa ggcctaattc tagtatgtat
     6121 gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac
     6181 tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat
     6241 ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag
     6301 cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg
     6361 ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat
     6421 tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt
     6481 gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt
     6541 gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag
     6601 gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat
     6661 gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa
     6721 catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac
     6781 aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt
     6841 tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt
     6901 tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa
     6961 aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca
     7021 taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt
     7081 cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac
     7141 ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg
     7201 cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc
     7261 tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac
     7321 atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata
     7381 aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt
     7441 ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg
     7501 atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt
     7561 ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg
     7621 ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct
     7681 gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa
     7741 atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag
     7801 gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga
     7861 gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct
     7921 gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga
     7981 gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag
     8041 cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt
     8101 acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg
     8161 cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc
     8221 ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt
     8281 tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca
     8341 aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca
     8401 aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa
     8461 acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg
     8521 cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga
     8581 agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt
     8641 gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg
     8701 tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt
     8761 ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa
     8821 ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg
     8881 aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat
     8941 tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac
     9001 tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg
     9061 tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt
     9121 ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg
     9181 gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg
     9241 agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt
     9301 cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg
     9361 ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg
     9421 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
     9481 actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca
     9541 aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag
     9601 tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact
     9661 tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat
     9721 attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac
     9781 cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt
     9841 tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt
     9901 tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta
     9961 tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta atttaatatt
    10021 atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac
    10081 tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat
    10141 aatagtgttg tttatatata tcaaaaataa caactataat aataagaata catttaattt
    10201 agaaaatgct tggatttcac tggaactaga attaattcgg ctgctgctct aaacgacgca
    10261 tttcgtactc caaagtacga attttttccc tcaagctctt attttcatta aacaatgaac
    10321 aggacctaac gcacagtcac gttattgttt acataaatga ttttttttac tattcaaact
    10381 tactctgttt gtgtactccc actggtatag ccttctttta tcttttctgg ttcaggctct
    10441 atcactttac taggtacggc atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt
    10501 gcaggtgcag ccttccactg cgaatcatta aagtgggtat cacaaatttg ggagttttca
    10561 ccaaggctgc acccaaggct ctgctcccac aattttctct taatagcaca cttcggcacg
    10621 tgaattaatt ttactccagt cacagctttg cagcaaaatt tgcaatattt catttttttt
    10681 tattccacgt aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct
    10741 caacaagcaa acgtgcactg aatttaagtg tatacttcgg taagcttcgg ctatcgacgg
    10801 gaccacctta tgttatttca tcatg
//
