LOCUS       sequence-22576-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Hirota J, Omura M, Mombaerts P
  TITLE     Differential impact of Lhx2 deficiency on expression of class I and 
            class II odorant receptor genes in mouse.
  JOURNAL   Mol Cell Neurosci. 2007 Apr . 34(4):679-88.
  PUBMED    17350283
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci. 2007 Apr . 34(4):679-88."
COMMENT     Sequence Label: pMOR31-8-ISH
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(650..668)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(651..668)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(686..702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(686..702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(699..721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    710..726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(734..740,741..758,759..764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    779..800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt ggaccggttt
       61 atggcaggag ggatggatac atagagattt gcaaacagta tatggaagga gtgggggata
      121 ttgtgaccaa aacgatggac aaggacagaa aatatggctg gtgtgtagaa tataagaatg
      181 acacagacat gggaaccaca tgtgctaagg gctttcttgc gggcatcctg ggagggaagg
      241 ttaaagacag cacgaagaat aacagtgtag gagatgccaa tcaagatcag atctgacatg
      301 acagtcatta ttggtactgc aaagccatac cagatattga tggagatgtc agcacaagcc
      361 agacgagcaa tgcctatgtg ctcacagtat gtgtgaggaa tgacgagctc tcggcagaaa
      421 ggcagtcgtt ttagcaagaa cacacatggg aagatgatac aaaagctcct gctgataatt
      481 gccaccacaa tcttaataac aatctgatgg gtgaggatgg tggtatatct taaggggaag
      541 cagatagcca cataccgatc cagcgacata atcactagtg aattcgcggc cgcctgcagg
      601 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
      661 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      721 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      781 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttt
//
