LOCUS       sequence-22596-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Ishii T, Mombaerts P
  TITLE     Expression of nonclassical class I major histocompatibility genes 
            defines a tripartite organization of the mouse vomeronasal system.
  JOURNAL   J Neurosci. 2008 Mar 5. 28(10):2332-41.
  PUBMED    18322080
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Mar 5. 28(10):2332-41."
COMMENT     Sequence Label: M10.2 Southern Blot 5'-external probe
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(615..633)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(616..633)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(651..667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(651..667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(664..686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    675..691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(699..705,706..723,724..729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    744..765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgccatgggc ggccgcggga attcgattac ctgctctggg tctaagctga agaggcaggg
       61 tctcaatgtg ccccacccac aggctcacac tggctgcaga ctttcaatac tgtgattttg
      121 aagcatggca cgttggagcc ccggatcatc caggtcggct atgtagactc catacagtat
      181 cagggatttg acagcaaaga accgactgca aggatgcaac ctcgggctgc atggatggag
      241 caggagccac ctgaatattg ggagaaggag acagcacaag tcctgcgctt gtcactgaca
      301 aacgaaagac ttcttaggta catgatgatc tacaatgaac agagcaatga gggtaagtca
      361 tctcagggct gaggtcataa gccctcaatg tccccctggt ttggcccaat aagctccact
      421 ctaggtcccc aaatcataga ttcagccata gtcaaaggga cccctggaga ccttgacctg
      481 agagaaccac agactgtgat ctggttaggg gtttagttgg ggacaaatcc cgggaatcac
      541 tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg
      601 catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg
      661 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      721 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      781 ctgcccgctt tccagt
//
