LOCUS       sequence-22598-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Ishii T, Mombaerts P
  TITLE     Expression of nonclassical class I major histocompatibility genes 
            defines a tripartite organization of the mouse vomeronasal system.
  JOURNAL   J Neurosci. 2008 Mar 5. 28(10):2332-41.
  PUBMED    18322080
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Mar 5. 28(10):2332-41."
COMMENT     Sequence Label: pISH-H2-Q1-alpha3-domain
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(296..314)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(297..314)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(332..348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(332..348)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(345..367)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    356..372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(380..386,387..404,405..410))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    425..446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(563..580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgccatggc ggccgcggga attcgattgg catgtgtaat tctgctcctt cccaagaggc
       61 accaccacag ctgcccactt ctggaaggtt ccatcccctg caggcctggt ctccacaagc
      121 tccatgtcct gggtcagctc ctccccattc aactgccagg tcagggtgat gtcagcaggg
      181 tagaagccca gggcccagca cctcagggtg acatcaatca ctagtgaatt cgcggccgcc
      241 tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat
      301 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      361 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      421 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      481 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      721 cgttgctggc gtttttccat
//
