LOCUS       sequence-22599-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Ishii T, Mombaerts P
  TITLE     Expression of nonclassical class I major histocompatibility genes 
            defines a tripartite organization of the mouse vomeronasal system.
  JOURNAL   J Neurosci. 2008 Mar 5. 28(10):2332-41.
  PUBMED    18322080
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Mar 5. 28(10):2332-41."
COMMENT     Sequence Label: pISH-bovine-tau
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(631..649)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(632..649)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(667..683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(667..683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(680..702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    691..707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(715..721,722..739,740..745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gccgccatgg gcggccgcgg gaattcgatt gcgaggtctt cggggtggga gtggtttttg
       61 ctggaatccg ggtggcgttg gcctggcctt tctggcctgg aggggctgct ccccggggtg
      121 tggcaatctt cgttccaggc ttaccatccg cccccttggt ttttttgtca tcgggtccag
      181 tcccatcttt gcctttactg accatgcgag cttgggtcac gtgtccggca gcttggtctt
      241 ccaggttgga cgtgtcgcca atgcctgctt cttcagctgc ggttccttct gggatctccg
      301 cgggggcctg agcggccgcc tgctcaccgg gggctccctc atccactaag ggtgctgtcg
      361 catcttccgc cgtcggagtg ctcttagcat cagaggtttc agagcctggt tcctcagatc
      421 catcatcggc cggggtctgc aggggagact ctttcaggcc ggggtccatg tcaccctcct
      481 ggtcttgcag ggtgtagtcc ccctgagcat gatcttccat cacgtcgaac tcctggcggg
      541 gctcagccat aatcactagt gaattcgcgg ccgcctgcag gtcgaccata tgggagagct
      601 cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta
      661 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      721 acgagccgga agcataaagt gtaaagcctg gggtggctaa tgagtgagct aactca
//
