LOCUS       sequence-22603-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Ishii T, Hirota J, Mombaerts P
  TITLE     Combinatorial coexpression of neural and immune multigene families 
            in mouse vomeronasal sensory neurons.
  JOURNAL   Curr Biol. 2003 Mar 4. 13(5):394-400.
  PUBMED    12620187
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Mar 4. 13(5):394-400."
COMMENT     Sequence Label: pISH-H2-M9
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(704..722)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(705..722)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(740..756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(740..756)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(753..775)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    764..780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgccatgggc ggccgcggga attcgatttg aagaactttg aatcccagcc tctcctcctg
       61 ctgttcatgg tcaccttggt catcgcaaag catccaaatg gttcacacac tctgagattt
      121 gtgtccacct tcttatcatg gcctcgacat ctggagctgc agtttatctt tctcatctat
      181 gtggatgaaa cacagatcat gggattcaac agtatatcag agagtcagag gatggagagt
      241 cgggtaccat ggttgaatga gctaaatgca gagttttggg agttggcaac acaggatgtc
      301 ctgaaagaaa aaagcttcgt gacaggaatt atgaacaaat tgctccacat ctataatgat
      361 agcatgactg gatatcacat aatccaggaa acatatggct gccaagtcaa gcagagaacg
      421 tacttcagtc acgccttcat ggaattatta ttcgatacac atgattacat cacactgaat
      481 gaggatctgc aaacatggag agcggttggc aaggcagctg agatagttaa ggaagagtgg
      541 gagaaaataa atcttgtaaa gtcgtcaaag tctttcctgc tgggtgcatg tgtggagggg
      601 ctccttcaat acctgaactt tggaatcact agtgaattcg cggccgcctg caggtcgacc
      661 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
      721 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      781 ttcacacaac atacgagccg gaaagcataa gtgtaaagcc tggg
//
