LOCUS       sequence-22605-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Ishii T, Hirota J, Mombaerts P
  TITLE     Combinatorial coexpression of neural and immune multigene families 
            in mouse vomeronasal sensory neurons.
  JOURNAL   Curr Biol. 2003 Mar 4. 13(5):394-400.
  PUBMED    12620187
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Mar 4. 13(5):394-400."
COMMENT     Sequence Label: pISH-H2-M10.1
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(644..662)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(645..662)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(680..696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(693..715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    704..720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(728..734,735..752,753..758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    773..794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattctgaa cactattgtg caggatcaca
       61 ctggctgcag actttcaaca ttgtaatttt ggagcctggc atgttggagc cccggttcat
      121 ccaggtcagc tatgtagact ccatacagta tcagggattt gacagcaaag acccatctgc
      181 agggatgcaa cctcgagctg catggataga gctggagcca cctgaatatt gggaaaagga
      241 gacatcaaga gttctggaat tgtcacaggt agagagacaa gttcttcggt taatggtgaa
      301 aaaaaatgga cacaaaatgg atggttatca caccctgcag gaagtatatg gttgcaatgt
      361 ggctaatgat gggagcttcc tcggcggtca ctaccgactc acctactatg gctatgatta
      421 cttaacccta aatgaggacc tgagctctcg gactgcagag ggcaagggag ctcaacacgc
      481 gaagagcagg tgggaggatg caggtgaagc aaaacggagg aagacttacc tgcaagggga
      541 gtgtgtacaa aggcttctta gataatcact agtgaattcg cggccgcctg caggtcgacc
      601 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
      661 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      781 gctaactcac attaattgcg ttgcgctcac tg
//
