LOCUS       sequence-22606-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Ishii T, Hirota J, Mombaerts P
  TITLE     Combinatorial coexpression of neural and immune multigene families 
            in mouse vomeronasal sensory neurons.
  JOURNAL   Curr Biol. 2003 Mar 4. 13(5):394-400.
  PUBMED    12620187
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Mar 4. 13(5):394-400."
COMMENT     Sequence Label: pISH-H2-M10.2
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(638..656)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(639..656)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(674..690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(687..709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    698..714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(722..728,729..746,747..752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ccgccatggc ggccgcggga attcgattga ccaggcatct aagaagcctt tctgtgcact
       61 cccctagcag gtaggtcctc cagccttccg ctgcgcctac attcaccaag tctgtcttca
      121 ggaacccagc tgcctctccc tgtgctgtcc aggaactcag gtcctcattc aggttgatga
      181 actcatagcc gtagtaggtg agccggtaga gaccgccgag gaagctccca tcattatcca
      241 cattgcagcc aaacacttcc tgcagggtgt gatattcctc attgctctgt tcattgtaga
      301 tcatcatgta cctaagaagt ctttcgtttg tcagtgacaa gcgcaggact tgtgctgtct
      361 ccttctccca atattcaggt ggctcctgct ccatccatgc agcccgaggt tgcatccttg
      421 cagtcggttc tttgctgtca aatccctgat actgtatgga gtctacatag ccgacctgga
      481 tgatccgggg ctccaacgtg ccatgcttca aaatcacagt attgaaagtc tgcagccagt
      541 gtgagcctac aggatagaat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg
      601 gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct
      661 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      721 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctact
      781 cacattaatt gcgttgcgct cact
//
