LOCUS       sequence-22607-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Ishii T, Hirota J, Mombaerts P
  TITLE     Combinatorial coexpression of neural and immune multigene families 
            in mouse vomeronasal sensory neurons.
  JOURNAL   Curr Biol. 2003 Mar 4. 13(5):394-400.
  PUBMED    12620187
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Mar 4. 13(5):394-400."
COMMENT     Sequence Label: pISH-H2-M10.3
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(627..645)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(628..645)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(663..679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(663..679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(676..698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    687..703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(711..717,718..735,736..741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    756..777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccgccatggc ggccgcggga attcgattaa gaagcctttg tacacactcc cctcgaaggt
       61 aagtcttcca cctttctgcc tcactcatat tctcccacct gttcttcatg tattcagctc
      121 ccttgccctc tgcagtccag gagctcaggt cctcattcag ggttaagtaa tcgtagccat
      181 agtaggtgag ccggaagtga ccatgaagga agcgcccatc agtagccaca ttgcagccat
      241 atacttcctg cagggtgtga taatcgttca ttctatgttc atatttttct atcatgaagt
      301 aaagaattcg tctttctatc tgtgacaata gcagaatttt tgttgtctcc acgttccaat
      361 acttaggtag ttcctgctcc atccatgcag cccgaggttt caaaatagcc attggttctt
      421 tgctgtcaaa tccttgatac tgtatagagt ctacatagcc aatgtggatg aaccgggaat
      481 taatcatgcc aggctccaaa aatgcaatgt tgaaagtctg cagccagtgt gagccttcac
      541 gatactaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca
      601 acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca
      661 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      721 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      781 gcgttgcgct cactgcccgc tttccagt
//
