LOCUS       sequence-22608-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Ishii T, Hirota J, Mombaerts P
  TITLE     Combinatorial coexpression of neural and immune multigene families 
            in mouse vomeronasal sensory neurons.
  JOURNAL   Curr Biol. 2003 Mar 4. 13(5):394-400.
  PUBMED    12620187
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Mar 4. 13(5):394-400."
COMMENT     Sequence Label: pISH-H2-M10.4
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(620..638)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(621..638)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(656..672)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(656..672)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(669..691)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    680..696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(704..710,711..728,729..734))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    749..770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggccgcggg aattcgattg ggctcacact ggctgaagac tttcaggatt gtaataatgg
       61 agcctggcat attggagccc cggttcatcc aggtcagcta tgtagactcc atacagtatc
      121 agggattcga cagcagatca gagactgtgg ggatgcaacc tcgggctgct tggatgaagc
      181 aggagccacc tgaatattgg aagaatgaga cagaacatgc catgggcgcg tcactgttag
      241 caagacgaac tcttatatac atggtgactg aaaataataa caagaagaat gactatcaca
      301 ccctgcagga agtgtttggc tgcaatgtgg cacatgatgg gagcttcctt ggtggtcact
      361 acgggctcac ctactatggc tatgattaca tcatcctgaa tgaggacctg aactcttgga
      421 ctacagaggg aaaagtaggt ggaaaattta atccagaccg gaagcaagga agcgtgacag
      481 aaggttggag gacttacctg aaaggggagt gcacagaaag gcttcttaga tacatggaca
      541 atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt
      601 ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
      661 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      721 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      781 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcg
//
