LOCUS       sequence-22609-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Ishii T, Hirota J, Mombaerts P
  TITLE     Combinatorial coexpression of neural and immune multigene families 
            in mouse vomeronasal sensory neurons.
  JOURNAL   Curr Biol. 2003 Mar 4. 13(5):394-400.
  PUBMED    12620187
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Mar 4. 13(5):394-400."
COMMENT     Sequence Label: pISH-H2-M10.5
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(646..664)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(647..664)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(682..698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(682..698)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(695..717)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    706..722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(730..736,737..754,755..760))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    775..796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctccgggccg ccatggcggc cgcgggaatt cgattgacca ggtatctaag aagcctttcc
       61 acgcacactc cttgcaggta agtcttccac ctttctgcat cacctccacc gtcccacctg
      121 ctcttcacat attcagctgc gtttccatgt gcaatccagg agctcaggtt ctcattcagg
      181 tataagtcat catagccata gtaggtgagc cggaagtgac cgccgaggaa gctcccatca
      241 ttagccacat tgcagccata cacttcctgc agggtgtgat aatcgtccat tctttgttca
      301 tttttgttca ccatgaagtg aagaattcgc ctgtctacct gtgacaattc cgcaatgctt
      361 gctgtctcac tggcccaata tgtcattggc acctgctcca tccatgcaac ccgaggtagc
      421 aaaactgcaa ttggttcttt gctgtcatat ctctgatact ctatagagtc tacatagcca
      481 atgtggatga accgggaatt aaccatgcca ggctccaaaa tcacagcgtt gaaagtctgc
      541 agccagtgtg agcctacacg atactaatca ctagtgaatt cgcggccgcc tgcaggtcga
      601 ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct
      661 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      721 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      781 gagctaactc acattaattg cgttgcgctc actgc
//
