LOCUS       sequence-22610-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ishii T, Hirota J, Mombaerts P
  TITLE     Combinatorial coexpression of neural and immune multigene families 
            in mouse vomeronasal sensory neurons.
  JOURNAL   Curr Biol. 2003 Mar 4. 13(5):394-400.
  PUBMED    12620187
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Mar 4. 13(5):394-400."
COMMENT     Sequence Label: pISH-H2-M10.6
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(622..640)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(623..640)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(658..674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(658..674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(671..693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    682..698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(706..712,713..730,731..736))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    751..772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tggcggccgc gggaattcga ttggctcaca ctggctgcag attttcaaca ttgtgatttt
       61 ggagcctggc atgttggagg cccggttcat ccaggtcgga tacgtagact ccatacagtt
      121 tcagggattc gacagcaaag aaccaaatgc aaggatgcaa cctcgggctg cttggatgaa
      181 gcaggagcca cctgaatatt gggagaaaga gacagcaaaa gccctgagct tctcactgtc
      241 aggaagacga attcttaagt acatgatgtc tgcaaataaa gacaagaata atggttatca
      301 caccctgcag gaagtgtatg gctgcaacgt ggcaaatgat gggagcttcc tcgaaggtca
      361 ctaccggctc acatactatg gatatgattc cttaatcctg aatgaggacc tgaactcttg
      421 gactgcagag ggtgaagtag gtgaaaaatt caagacatac caggagcaag gaggcatgac
      481 agaaagttgg aggacttacc tgctagggga atgcgtggaa aggcttctta gatacatggt
      541 caatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg
      601 ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc
      661 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      721 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      781 gcgctcactg cccgctttcc agtcgggaaa actgtcgtgc cagctgcatt aatgaatcgg
      841 ccaacgcgcg g
//
