LOCUS       sequence-226138-        4149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Part. CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.018
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..24,25..27,28..2514,2515..2517,2518..4101)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc
       61 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac
      121 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg
      181 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat
      241 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt
      301 gaagagtctt ttttggtgga agaagataag aagcatgaac gtcatcctat ttttggaaat
      361 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa
      421 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg
      481 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg
      541 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt
      601 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga
      661 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc
      721 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat
      781 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa
      841 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta
      901 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg
      961 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa
     1021 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt
     1081 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa
     1141 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag
     1201 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct
     1261 attttgagac gacaagaaga tttttatcca tttttaaaag acaatcgtga gaagattgaa
     1321 aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt
     1381 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt
     1441 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat
     1501 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat
     1561 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca
     1621 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt
     1681 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca
     1741 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt
     1801 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta
     1861 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac
     1921 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt
     1981 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat
     2041 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt
     2101 ttgacattta aagaagatat tcaaaaagca caagtgtctg gacaaggcga tagtttacat
     2161 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta
     2221 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt
     2281 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg
     2341 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt
     2401 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac
     2461 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatgccatt
     2521 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat
     2581 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac
     2641 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg
     2701 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg
     2761 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact
     2821 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa
     2881 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac
     2941 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat
     3001 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg
     3061 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat
     3121 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct
     3181 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc
     3241 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag
     3301 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct
     3361 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat
     3421 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa
     3481 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt
     3541 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat
     3601 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa
     3661 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat
     3721 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag
     3781 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt
     3841 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca
     3901 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc
     3961 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa
     4021 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat
     4081 ttgagtcagc taggaggtga cgccgccaat aacatcgttt cctttaagcg ggtagccatt
     4141 gccgcctaa
//
