LOCUS       sequence-226142-        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Part. CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.027
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(104..123)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             115..930
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             115..930
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(188..207)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aagcaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
       61 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
      121 catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat
      181 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      241 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      301 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      361 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      421 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      481 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      541 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
      601 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
      661 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
      721 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
      781 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
      841 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
      901 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
      961 cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga acttttgctg
     1021 agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa
     1081 agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca
     1141 ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac
     1201 gaggcagacc tcagacct
//
