LOCUS       sequence-22622-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Kanda T, Sullivan KF, Wahl GM
  TITLE     Histone-GFP fusion protein enables sensitive analysis of chromosome 
            dynamics in living mammalian cells.
  JOURNAL   Curr Biol. 1998 Mar 26. 8(7):377-85.
  PUBMED    9545195
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1998 Mar 26. 8(7):377-85."
COMMENT     Sequence Label: pCLNR-H2BG
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..424
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      437..446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(443..445,446..448,449..907)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(488..509)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(749..768)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtgtgcnct ccagcttcga attctgcagt cgacggtacc gccaccatgc cagagccagc
       61 gaagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa
      121 aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt
      181 tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc
      241 gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa
      301 caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg
      361 ggagttggcc aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc
      421 taaggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      481 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      541 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      601 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      661 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      721 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      781 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      841 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      901 catggccgac agcagagacg ctca
//
