LOCUS       sequence-226249-        4582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon mutagenesis vector for Bacteroides thetaiotaomicron. 
            Compatible with Insertion Sequencing (INSeq)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Goodman AL, McNulty NP, Zhao Y, Leip D, Mitra RD, Lozupone CA, 
            Knight R, Gordon JI
  TITLE     Identifying genetic determinants needed to establish a human gut 
            symbiont in its habitat.
  JOURNAL   Cell Host Microbe. 2009 Sep 17;6(3):279-89. doi: 
            10.1016/j.chom.2009.08.003.
  PUBMED    19748469
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2009.08"; journalName: "Cell Host Microbe"; date: 
            "2009-09-17- 17"; volume: "6"; issue: "3"; pages: "279-89"
COMMENT     Sequence Label: pSAM_Bt
FEATURES             Location/Qualifiers
     source          1..4582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1034..1052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2142..2228
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2320..2347
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      3807..4195
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            4310..4419
                     /note="incP origin of transfer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt cacgcgtcct cggtacctaa
     1081 caggttggat gataagtccc cggtcttcgt atgccgtctt ctgcttggcg cgccctcgag
     1141 caattgccga ccgaattttt atgtcgtaaa gaggggcttt gcagggggtg gactcagaaa
     1201 gatgagaata gatgactatt gtagttgaaa cacatagaaa gttgctgata tacagaccga
     1261 tacgcatatc gggatgaacc atgagtacgt tcttttctca aaaaacataa atattcgaaa
     1321 agagatgcaa taaattaagg agaggttata atgaacaaag taaatataaa agatagtcaa
     1381 aattttatta cttcaaaata tcacatagaa aaaataatga attgcataag tttagatgaa
     1441 aaagataaca tctttgaaat aggtgcaggg aaaggtcatt ttactgctgg attggtaaag
     1501 agatgtaatt ttgtaacggc gatagaaatt gattctaaat tatgtgaggt aactcgtaat
     1561 aagctcttaa attatcctaa ctatcaaata gtaaatgatg atatactgaa atttacattt
     1621 cctagccaca atccatataa aatatttggc agcatacctt acaacataag cacaaatata
     1681 attcgaaaaa ttgtttttga aagttcagcc acaataagtt atttaatagt ggaatatggt
     1741 tttgctaaaa tgttattaga tacaaacaga tcactagcat tgctgttaat ggcagaggta
     1801 gatatttcta tattagcaaa aattcctagg tattatttcc atccaaaacc taaagtggat
     1861 agcacattaa ttgtattaaa aagaaagcca gcaaaaatgg catttaaaga gagaaaaaaa
     1921 tatgaaactt ttgtaatgaa atgggttaac aaagagtacg aaaaactgtt tacaaaaaat
     1981 caatttaata aagctttaaa acatgcgaga atatatgata taaacaatat tagtttcgaa
     2041 caatttgtat cgctatttaa tagttataaa atatttaacg gctaaaaaca ataggccaca
     2101 tgcaactgtc tagaatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     2161 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     2221 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     2281 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     2341 gcctttttgc gtttctacct gcagggcgcg ccaagcagaa gacggcatac gaagaccggg
     2401 gacttatcat ccaacctgtt aggatcctga tctggaagaa gcaatgaaag ctgctgttaa
     2461 gtctccgaat caggtattgt tcctgacagg tgtattccca tccggtaaac gcggatactt
     2521 tgcagttgat ctgactcagg aataaattat aaattaaggt aagaagattg taggataagc
     2581 taatgaaata gaaaaaggat gccgtcacac aacttgtcgg cattcttttt tgttttatta
     2641 gttgaaaata tagtgaaaaa gttgcctaaa tatgtatgtt aacaaattat ttgtcgtaac
     2701 tttgcactcc aaatctgttt ttaacatatg gaaaaaaagg aatttcgtgt tttgataaaa
     2761 tactgttttc tgaagggaaa aaatacagtg gaagcaaaaa cttggcttga taatgagttt
     2821 ccggactctg ccccagggaa atcaacaata attgattggt atgcaaaatt caagcgtggt
     2881 gaaatgagca cggaggacgg tgaacgcagt ggacgcccga aagaggtggt taccgacgaa
     2941 aacatcaaaa aaatccacaa aatgattttg aatgaccgta aaatgaagtt gatcgagata
     3001 gcagaggcct taaagatatc aaaggaacgt gttggtcata tcattcatca atatttggat
     3061 atgcggaagc tctgtgcgaa atgggtgccg cgcgagctca catttgacca aaaacaacga
     3121 cgtgttgatg attctaagcg gtgtttgcag ctgttaactc gtaatacacc cgagtttttc
     3181 cgtcgatatg tgacaatgga tgaaacatgg ctccatcact acactcctga gtccaatcga
     3241 cagtcggctg agtggacagc gaccggtgaa ccgtctccga agcgtggaaa gactcaaaag
     3301 tccgctggca aagtaatggc ctctgttttt tgggatgcgc atggaataat ttttatcgat
     3361 tatcttgaga agggaaaaac catcaacagt gactattata tggcgttatt ggagcgtttg
     3421 aaggtcgaaa tcgcggcaaa acggccccac atgaagaaga aaaaagtgtt gttccaccaa
     3481 gacaacgcac cgtgccacaa gtcattgaga acgatggcaa aaattcatga attgggcttc
     3541 gaattgcttc cccacccgcc gtattctcca gatctggccc ccagcgactt tttcttgttc
     3601 tcagacctca aaaggatgct cgcagggaaa aaatttggct gcaatgaaga ggtgatcgcc
     3661 gaaactgagg cctattttga ggcaaaaccg aaggagtact accaaaatgg tatcaaaaaa
     3721 ttggaaggtc gttataatcg ttgtatcgct cttgaaggga actatgttga ataagcggcc
     3781 gccaccgcgg tggaggggaa ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa
     3841 ttgctttgag aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa
     3901 ccgttaaacc ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa
     3961 aaccttagag gctatttaag ttgctgattt atattaattt tattgttcaa acatgagagc
     4021 ttagtacgtg aaacatgaga gcttagtacg ttagccatga gagcttagta cgttagccat
     4081 gagggtttag ttcgttaaac atgagagctt agtacgttaa acatgagagc ttagtacgtg
     4141 aaacatgaga gcttagtacg tactatcaac aggttgaact gctgatcttc agatcctcta
     4201 cgccggacgc atcgtggccg gatcaattcc gtgataggtg ggctgccctt cctggttggc
     4261 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
     4321 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
     4381 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
     4441 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
     4501 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaacggaatt
     4561 gatccccctc gaattgacgc gt
//
