LOCUS       sequence-22626-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     Jon Epstein Lab Plasmids
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS-mPax3
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(415..431)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(416..432)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(460..478)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(499..515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(499..515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(512..534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    523..539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(547..553,554..571,572..577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    592..613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(730..747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggccgct ctagaactag tggatccccc gggccgggcc gcatcatcct gggcacagcg
       61 ccggccagcg tggtcatcct gggggcagct tcgctcgcaa attatatcca ggtgaaggcg
      121 agacgaaaaa gcgagcacag tgcggcgggt gaccctcggg aactctccgg atctcggaga
      181 gctcctcccc caaaaaaggc cagagaggga ggcggggcgc ggggaggggg gcgctaatag
      241 atccaagtcc acaggctcca gaggagtccc ttgagtgcag gatcccaagc ccagggcgga
      301 cgagtttggt gcgagtctag cagatcggcc cctcagctgg ggctactggc aaaggtgctc
      361 actgcaccgc gaagatccct tgaccgtaag acatgaattc gatatcaagc ttatcgatac
      421 cgtcgacctc gagggggggc ccggtaccag cttttgttcc ctttagtgag ggttaatttc
      481 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      661 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgngcgctc
      721 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      781 agctcac
//
