LOCUS       sequence-226276-        1266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Marker fusion of Bar:mCherry (without start codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1269R
FEATURES             Location/Qualifiers
     source          1..1266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..552
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="SPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQEW
                     TDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGST
                     LYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGFW
                     QLDFSLPVPPRPVLPVTEI"
     CDS             553..1260
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(695..713)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     963..982
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtaccagcc cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg
       61 gcggtctgca ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag
      121 ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg
      181 ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct acgcgggtcc ctggaaggca
      241 cgcaacgcct acgactggac ggccgaatcg accgtgtacg tctccccccg ccaccagcgg
      301 acgggactgg gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc
      361 aagagcgtgg tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg
      421 ctcggatatg ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat
      481 gacgtgggtt tctggcagct ggacttcagc ctgccggttc cgccccgtcc ggtcctgccc
      541 gtcaccgaga ttgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      601 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      661 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      721 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      781 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      841 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      901 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      961 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1021 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1081 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1141 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1201 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     1261 ggatcc
//
