LOCUS       sequence-226287-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry (without start and stop codons) in pGFL1010.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1259R
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..711
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(149..167)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     417..436
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtaccgtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
       61 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      121 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      181 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      241 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      301 cgcgtgatga acttcgagga cggtggcgtg gtgaccgtga cccaggactc ctccctgcag
      361 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      421 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      481 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      541 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      601 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      661 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatcc
//
