LOCUS       sequence-226288-        2901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence nptII from E. coli domesticated into pUPD2 with 
            AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA
            assembly for A. tumefaciens-mediated genetic transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB005
FEATURES             Location/Qualifiers
     source          1..2901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..796
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(56..75)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     666..685
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      838..895
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(919..936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1090..1678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1170..1189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1861..1955)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1976..2635)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2550..2569
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2797..2815
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2819..2862)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2865..2886
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
       61 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
      121 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
      181 ccaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
      241 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
      301 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
      361 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacaccg
      421 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
      481 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc ggatgcccga
      541 cggcgaggat ctcgtcgtga cccacggcga cgcctgcttg ccgaatatca tggtggaaaa
      601 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
      661 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
      721 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atagccttct atcgccttct
      781 tgacgagttc ttctgagctt tgagaccact catcgcgact agtagcggcc gctgcagtcc
      841 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      901 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1081 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1261 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1501 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     1861 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1921 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1981 ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     2041 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     2101 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2161 ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa
     2221 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2281 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag
     2341 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2401 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2461 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2521 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2581 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2641 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2701 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     2761 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     2821 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     2881 ctagagcgat gagtggtctc g
//
