LOCUS       sequence-22631-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Zhou BP, Deng J, Xia W, Xu J, Li YM, Gunduz M, Hung MC
  TITLE     Dual regulation of Snail by GSK-3beta-mediated phosphorylation in 
            control of epithelial-mesenchymal transition.
  JOURNAL   Nat Cell Biol. 2004 Oct . 6(10):931-40.
  PUBMED    15448698
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2004 Oct . 6(10):931-40."
COMMENT     Sequence Label: Flag Snail 6SA
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..50
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcagatccgc tagcgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc
       61 tggagctcca ccgcggtggc ggccgccacc atggattaca aggatgacga cgataagagc
      121 ccgggcggat ccccgcgctc tttcctcgtc aggaagccct ccgaccccaa tcggaagcct
      181 aactacagcg agctgcagga ctctaatcca gagtttacct tccagcagcc ctacgaccag
      241 gcccacctgc tggcagccat cccacctccg gagatcctca accccaccgc ctcgctgcca
      301 atgctcatct gggactctgt cctggcgccc caagcccagc caattgcctg ggcctccctt
      361 cggctccagg agagtcccag ggtggcagag ctgacctccc tgtcagatga ggacgctggg
      421 aaaggcgccc agccccccag cccacccgca ccggctcctg cgtccttctc cgcgacgtca
      481 gtcgcttcct tggaggccga ggcctatgct gccttcccag gcttgggcca agtgcccaag
      541 cagctggccc agctctctga ggccaaggat ctccaggctc gaaaggcctt caactgcaaa
      601 tactgcaaca aggaatacct cagcctgggt gccctcaaga tgcacatccg aagccacacg
      661 ctgccctgcg tctgcggaac ctgcgggaag gccttctcta ggccctggct gctacaaggc
      721 catgtccgga cccacactgg cgagaagccc ttctcctgtc cccactgcag ccgtgccttc
      781 gctgaccgct ccaacctgcg ggcccacctc cagacccact cagatgtcaa gaagtaccag
      841 tgccaggcgt gtgctcggac cttctcccga atgtccctgc tccacaagca ccaagagtcc
      901 ggctgctcag gatgtccccg ctga
//
