LOCUS       sequence-226346-        5147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ARL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Van Valkenburgh H, Shern JF, Sharer JD, Zhu X, Kahn RA
  TITLE     ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both 
            specific and shared effectors: characterizing ARL1-binding proteins.
  JOURNAL   J Biol Chem. 2001 Jun 22;276(25):22826-37. doi: 
            10.1074/jbc.M102359200. Epub 2001 Apr 12.
  PUBMED    11303027
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M102359200"; journalName: "J Biol Chem"; date: 
            "2001-06-22- 22"; volume: "276"; issue: "25"; pages: "22826-37"
COMMENT     Sequence Label: pJL1
FEATURES             Location/Qualifiers
     source          1..5147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             91..113
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(717..735)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      731..778
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1144..1149,1150..1166,1167..1172))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1199..1217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1285..1389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1390..1458,1459..2250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1608..1627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2421..3009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2910..2929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3163..3180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3195..3334
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3260..3279)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3420..3442
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3436..3627)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5085..5104
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact cactataggg
       61 agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacat
      121 atgggtggct ttttctcaag tatattttcc agtctgtttg gaactcggga aatgagaatt
      181 ttaattttgg gattagatgg agcaggaaaa accacaattt tgtacagatt acaagtggga
      241 gaagttgtta ctactatacc taccattgga tttaatgtag agacggtgac gtacaaaaac
      301 cttaaattcc aagtctggga tttaggagga cagacaagta tcaggccata ctggagatgt
      361 tactattcaa acacagatgc agtcatttat gtagtagaca gttgtgaccg agaccgaatt
      421 ggcatttcca aatcagagtt agttgccatg ttggaggaag aagagctgag aaaagccatt
      481 ttagtggtgt ttgcaaataa acaggacatg gaacaggcca tgacttcctc agagatggca
      541 aattcacttg ggttacctgc cttgaaggac cgaaaatggc agatattcaa aacgtcagca
      601 accaaaggca ccggccttga tgaggcaatg gaatggttag ttgaaacatt aaaaagcaga
      661 cagtgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      721 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      781 gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      841 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
      901 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
      961 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
     1021 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
     1081 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
     1141 cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc
     1201 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1261 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1321 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1381 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1441 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1501 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1561 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1621 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1681 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1741 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1801 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1861 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1921 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1981 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2041 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2101 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2161 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2221 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2281 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2341 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2401 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2461 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2521 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2581 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2641 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2701 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2761 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2821 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2881 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2941 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3001 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3061 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3121 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3181 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3241 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     3301 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     3361 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3421 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     3481 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     3541 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     3601 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     3661 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     3721 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     3781 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     3841 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     3901 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
     3961 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     4021 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     4081 catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga
     4141 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
     4201 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct
     4261 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     4321 aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca
     4381 taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcgagc
     4441 gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     4501 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     4561 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg
     4621 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
     4681 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
     4741 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
     4801 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
     4861 gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg
     4921 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     4981 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     5041 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     5101 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgat
//
