LOCUS       sequence-226358-        5223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ARL14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5223)
  TITLE     Kahn expression plasmids
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: rk17s
FEATURES             Location/Qualifiers
     source          1..5223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    114..138
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(739..763)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(816..834)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      830..877
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1243..1248,1249..1265,1266..1271))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1298..1316)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1384..1488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1489..1557,1558..2349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1707..1726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2520..3108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3009..3028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3262..3279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3294..3433
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3359..3378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3519..3541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3535..3726)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5188..5207
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
       61 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag atcacaagtt
      121 tgtacaaaaa agcaggctta gaaggagata gaaccatggg ttcgctgggt tctaaaaatc
      181 cgcaaaccaa acaagcccaa gttcttcttt tgggacttga ctcagctggg aagtctactc
      241 tcctttataa attaaagctt gctaaggata ttaccaccat ccctacaata ggtttcaatg
      301 tggaaatgat cgagttggaa aggaattttt cactcacagt ctgggatgtt ggaggacagg
      361 aaaaaatgag aactgtttgg ggctgttact gtgagaacac cgatgggctg gtgtatgttg
      421 tggacagtac agacaaacag cgactggaag agtctcagag acagtttgag cacattttga
      481 agaatgaaca cattaaaaat gtgcctgttg ttctattagc caacaaacaa gacatgcctg
      541 gagctctgac tgctgaggac atcaccagaa tgttcaaagt gaagaagctt tgcagtgacc
      601 ggaactggta tgtgcaaccc tgctgtgccc tcacagggga ggggctggcc caggggttca
      661 ggaaattaac tggatttgtg aagagccaca tgaaatcaag aggagacact ttggcgttct
      721 tcaagcagaa ctgataggac ccagctttct tgtacaaagt ggtgatgatc cggctgctaa
      781 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      841 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      901 atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg
      961 aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc
     1021 atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt
     1081 cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta
     1141 cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg
     1201 gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga
     1261 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     1321 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1381 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1441 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1501 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1561 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1621 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1681 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     1741 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1801 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1861 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1921 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1981 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     2041 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2101 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2161 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2221 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2281 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2341 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2401 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2461 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2641 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2881 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2941 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3001 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3061 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3301 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta
     3361 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     3421 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3481 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3541 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     3601 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     3661 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     3721 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     3781 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     3841 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     3901 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     3961 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     4021 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     4081 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     4141 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga
     4201 cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
     4261 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
     4321 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
     4381 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
     4441 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
     4501 ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
     4561 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     4621 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     4681 acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
     4741 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca
     4801 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
     4861 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
     4921 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
     4981 atcggtcgat cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     5041 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     5101 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     5161 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     5221 acc
//
