LOCUS       sequence-226388-        7145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn5 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn5-130
FEATURES             Location/Qualifiers
     source          1..7145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..711
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      728..822
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2204..2863
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2270..2289)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2928..3014
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4758..4776)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4844..4948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4949..5017,5018..5809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5167..5186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6048..6157
                     /note="incP origin of transfer"
     primer_bind     6711..6727
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6711..6727
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
       61 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      121 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      181 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
      241 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      301 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
      361 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
      421 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
      481 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
      541 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
      601 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
      661 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttaattagct
      721 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      781 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgct
      841 gagagatccc ctcataattt ccccaaagcg taaccatgtg tgaataaatt ttgagctagt
      901 agggttgcag ccacgagtaa gtcttccctt gttattgtgt agccagaatg ccgcaaaact
      961 tccatgccta agcgaactgt tgagagtacg tttcgatttc tgactgtgtt agcctggaag
     1021 tgcttgtccc aaccttgttt ctgagcatga acgcccgcaa gccaacatgt tagttgaagc
     1081 atcagggcga ttagcagcat gatatcaaaa cgctctgagc tgctcgttcg gctatggcgt
     1141 aggcctagtc cgtaggcagg acttttcaag tctcggaagg tttcttcaat ctgcattcgc
     1201 ttcgaataga tattaacaag ttgtttgggt gttcgaattt caacaggtaa gttagttgct
     1261 agaatccatg gctcctttgc cgacgctgag tagattttag gtgacgggtg gtgacaatga
     1321 gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag atttatacaa tagaatttgg
     1381 catgagattg gattgctttt agtcagcctc ttatagccta aagtctttga gtgactagat
     1441 gacatatcat gtaagttgct gataggtttc cagttttccg ctcctaggtc tgcatattgt
     1501 acttttcctc ttactcgact taaccagtac caacccagct tctcaacgga tttataccat
     1561 ggcactttaa agccagcatc actgacaatg agcggtgtgg tgttactcgg tagaatgctc
     1621 gcaaggtcgg ctagaaattg gtcatgagct ttctttgaac attgctctga aagcgggaac
     1681 gctttctcat aaagagtaac agaacgaccg tgtagtgcga ctgaagctcg caataccata
     1741 agccgttttt gctcacggat atcagaccag tcaacaagta caatgggcat cgtattgccc
     1801 gaacagataa agctagcatg ccaacggtat acagcgagtc gctctttgtg gaggtgacga
     1861 ttacctaaca atcggtcgat tcgtttgatg ttatgttttg ttctcgcttt ggttggcagg
     1921 ttacggccaa gttcggtaag agtgagagtt ttacagtcaa gtaaggcgtg gcaagccaac
     1981 gttaagctgt tgagtcgttt taagtgtaat tcggggcaga attggtaaag agagtcgtgt
     2041 aaaatatcga gttcgcacat tttgttgtct gattattgat ttttggcgaa accatttgat
     2101 catatgacaa gatgtgtatc taccttaact taatgatttt gataaaaatc attaggggat
     2161 tcatcagagc ttggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     2221 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     2281 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     2341 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     2401 gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga
     2461 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     2521 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     2581 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     2641 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     2701 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     2761 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2821 tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
     2881 ttggtgccct taaacgcctg gggtaatgac tctctagctt gaggcatcaa ataaaacgaa
     2941 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     3001 tgagtaggac aaatccgccg ctaggagctt gcggccgcca ggatatcgac ccaagtaccg
     3061 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgacgcgtca attcgagcgc
     3121 gtcaattcga gggccgcggc ctaggcggcc agatctgatc aagagacagt ctagctagaa
     3181 ttaattcgag tcgaccgatc ccgtacacaa gtagcgtcct gaacggaacc tttcccgttt
     3241 tccaggatct gatcttccat gtgacctcct aacatggtaa cgttcatgat aacttctgct
     3301 cttcatcgtg cggccgactg ggctaaatct gtgttctctt cggcggcgct gggtgatcct
     3361 cgccgtactg cccgcttggt taacgtcgcc gcccaattgg caaaatattc tggtaaatca
     3421 ataaccatct catcagaggg tagtgaagcc atgcaggaag gcgcttaccg atttatccgc
     3481 aatcccaacg tttctgccga ggcgatcaga aaggctggcg ccatgcaaac agtcaagttg
     3541 gctcaggagt ttcccgaact gctggccatt gaggacacca cctctttgag ttatcgccac
     3601 caggtcgccg aagagcttgg caagctgggc tctattcagg ataaatcccg cggatggtgg
     3661 gttcactccg ttctcttgct cgaggccacc acattccgca ccgtaggatt actgcatcag
     3721 gagtggtgga tgcgcccgga tgaccctgcc gatgcggatg aaaaggagag tggcaaatgg
     3781 ctggcagccg ccgcaactag ccggttacgc atgggcagca tgatgagcaa cgtgattgcg
     3841 gtctgtgacc gcgaagccga tattcatgct tatctgcagg acaaactggc gcataacgag
     3901 cgcttcgtgg tgcgctccaa gcacccacgc aaggacgtag agtctgggtt gtatctgtac
     3961 gaccatctga agaaccaacc ggagttgggt ggctatcaga tcagcattcc gcaaaagggc
     4021 gtggtggata aacgcggtaa acgtaaaaat cgaccagccc gcaaggcgag cttgagcctg
     4081 cgcagtgggc gcatcacgct aaaacagggg aatatcacgc tcaacgcggt gctggccgag
     4141 gagattaacc cgcccaaggg tgagaccccg ttgaaatggt tgttgctgac cagcgaaccg
     4201 gtcgagtcgc tagcccaagc cttgcgcgtc atcgacattt atacccatcg ctggcggatc
     4261 gaggagttcc ataaggcatg gaaaaccgga gcaggagccg agaggcaacg catggaggag
     4321 ccggataatc tggagcggat ggtctcgatc ctctcgtttg ttgcggtcag gctgttacag
     4381 ctcagagaaa gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
     4441 gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     4501 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     4561 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     4621 tggggcgccc tctgggaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     4681 gccaaggatc tgatggcgca ggggatcaag atctgatcgg tcgatcgaat taattcccaa
     4741 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4801 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5161 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5881 ggtgaagatc cggggaattg gggatcaatt ccgttttccg ctgcataacc ctgcttcggg
     5941 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     6001 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     6061 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     6121 gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag
     6181 ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcacggaa
     6241 ttgatccggc cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg
     6301 atagtacgta ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc
     6361 tctcatgttt aacgaactaa accctcatgg ctaacgtact aagctctcat gtttcacgta
     6421 ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa
     6481 ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta
     6541 acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa
     6601 gcaattttga gtgacacagg aacacttaac ggctgacatg ggaattaatt ctgactctta
     6661 tacacaagtg cggccctcga attgacgcgc tccaccgcgg tggcggccgc tctagaacta
     6721 gtggatcccc cgggctgcag gaattcgata tccataaact gccaggaatt ggggatcgga
     6781 ttcgagctcg cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac
     6841 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     6901 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     6961 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     7021 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     7081 aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatacta gtggaggaag
     7141 aaaaa
//
