LOCUS       sequence-226400-        2837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with
            GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA
            assembly for A. tumefaciens-mediated genetic transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB007
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      774..831
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(855..872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1026..1614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1106..1125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1797..1891)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1912..2571)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2486..2505
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2733..2751
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2755..2798)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2801..2822
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagtggcgc atgcggacag acggacggac gcagagagaa gggctgagta ataagcgcca
       61 ctgcgccaga cagctctggc ggctctgagg tgcagtggat gattattaat ccgggaccgg
      121 ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa gaatctattg
      181 catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg agaatgtgaa
      241 gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac agaagtccaa
      301 ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta ggtagagcga
      361 gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg tccaaatatc
      421 gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga gctggccagc
      481 ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca ctcgacctgc
      541 tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg tgtgtcggcg
      601 gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct gctctcccca
      661 ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat cttcccatcc
      721 aagaaccttt aaaatgtgag accactcatc gcgactagta gcggccgctg cagtccggca
      781 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
      841 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      901 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      961 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1021 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1381 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1441 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1501 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1561 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1621 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1681 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1741 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
     1801 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1861 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     1921 gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     1981 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     2041 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     2101 atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa
     2161 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     2221 tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg
     2281 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     2341 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     2401 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     2461 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     2521 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2581 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2641 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
     2701 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
     2761 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag
     2821 agcgatgagt ggtctcg
//
