LOCUS       sequence-226418-       11192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/lox flip-out construct for marking cell clones in Tribolium 
            castaneum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Gilles AF, Schinko JB, Schacht MI, Enjolras C, Averof M
  TITLE     Clonal analysis by tunable CRISPR-mediated excision.
  JOURNAL   Development. 2019 Jan 4;146(1). pii: dev.170969. doi: 
            10.1242/dev.170969.
  PUBMED    30552128
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2019-01-4"; volume: "146(1). pii: dev.170969.
            doi"; pages: " 10.1242/dev.170969"
COMMENT     Sequence Label: pBac(3xP3-gTc'v; pUb:lox-mYFP-lox-H2BmCherry)
FEATURES             Location/Qualifiers
     source          1..11192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   20..54
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(2403..2436)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             join(2509..2511,2512..2514,2515..3228)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2554..2575)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2815..2834)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3162..3183
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3351..3472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3479..3512)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             3896..4603
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4041..4059)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4309..4328
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4613..4669
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    4739..4860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7663..7725
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8414..8431)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8414..8430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8423..8445)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8639..8658)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8758..8780
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8818..8836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8904..9008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9009..9077,9078..9869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9227..9246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10040..10628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10529..10548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10782..10799
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10916..10937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10952..10957,10958..10975,10976..10982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10990..11006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10995..11017
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11014..11030
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11014..11030
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcgcaaat cttttttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct
       61 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct
      121 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt
      181 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa
      241 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt
      301 atatatatat tttcttgtta tagatatcgt gactaatata taataaaatg ggtagttctt
      361 tagacgatga gcatatcctc tctgctcttc tgcaaagcga tgacgagctt gttggtgagg
      421 attctgacag tgaaatatca gatcacgtaa gtgaagatga cgtccagagc gatacagaag
      481 aagcgtttat agatgaggta catgaagtgc agccaacgtc aagcggtagt gaaatattag
      541 acgaacaaaa tgttattgaa caaccaggtt cttcattggc ttctaacaga atcttgacct
      601 tgccacagag gactattaga ggtaagaata aacattgttg gtcaacttca aagtccacga
      661 ggcgtagccg agtctctgca ctgaacattg tcagatctca aagaggtccg acgcgtatgt
      721 gccgcaatat atatgaccca cttttatgct tcaaactatt ttttactgat gagataattt
      781 cggaaattgt aaaatggaca aatgctgaga tatcattgaa acgtcgggaa tctatgacag
      841 gtgctacatt tcgtgacacg aatgaagatg aaatctatgc tttctttggt attctggtaa
      901 tgacagcagt gagaaaagat aaccacatgt ccacagatga cctctttgat cgatctttgt
      961 caatggtgta cgtctctgta atgagtcgtg atcgttttga ttttttgata cgatgtctta
     1021 gaatggatga caaaagtata cggcccacac ttcgagaaaa cgatgtattt actcctgtta
     1081 gaaaaatatg ggatctcttt atccatcagt gcatacaaaa ttacactcca ggggctcatt
     1141 tgaccataga tgaacagtta cttggtttta gaggacggtg tccgtttagg atgtatatcc
     1201 caaacaagcc aagtaagtat ggaataaaaa tcctcatgat gtgtgacagt ggtacgaagt
     1261 atatgataaa tggaatgcct tatttgggaa gaggaacaca gaccaacgga gtaccactcg
     1321 gtgaatacta cgtgaaggag ttatcaaagc ctgtgcacgg tagttgtcgt aatattacgt
     1381 gtgacaattg gttcacctca atccctttgg caaaaaactt actacaagaa ccgtataagt
     1441 tcgagatcgg ccggcctttt ctttgtccca aatgaccctt ttacataatt tattattgtc
     1501 gtccgtattt acatgataaa caagactcta gaacaacaac gataaggata attctttgta
     1561 aacaaattaa ttcgtttcaa aattgaacag acgaatatgg cattccatgc aggaattaat
     1621 gggtggttta tttgaaacgt ttgaaaagca taaattggga aataaattaa aatttgaaaa
     1681 ataaaaaagg gcgtaattcc aaaacaaaga aaacagctga tcgataaagc tgacgacgta
     1741 gataaaaatc agctgacatg tcaccaacaa ctgctggcca aagtccctaa aatcttgttc
     1801 aatcaaaagc aattttctta atacctgaaa cattgtttag accatgatta accgaattgg
     1861 cattaaacta acagcggcta acgtttgtgc ataaattttt gattaaagag gacgtttttt
     1921 ggataattac tcgaaaattc tagaaagtgt actttgggtt cgagattaaa aaggagtggg
     1981 ggaagccgtc cgtataaaag cggttccaca gctgcattcg ccatattgtc tgagcaaaag
     2041 tggtgaaata caaaagcgat ttaattaaaa ttgtgaattt tcctagccgt aaaggtatgt
     2101 attttaattt catcatgaat tattcgattg caagaagcgc agaaagtcgc aattttgtga
     2161 attttttcgc atgacgcaat aacatttgtt tgaaaaaaca tcgccctagt gacgtgtttt
     2221 ttgtttaaat tttaacaaaa ttaattttgt aaatttgtcg atctgttcaa aaccaaacca
     2281 catagtgagt aatgtgtttt ttcggtttat tcagtgcgta attctttgtt caacgaaatc
     2341 caatatggcg gcctggaaat ttgctttgtc acaaaagtaa ttttttgtgt cgttgcagat
     2401 ctataacttc gtatagtata ccttatacga agttattgcg atcgcaaaat gctgtgctgt
     2461 atgagaagaa ccaaacaggt tgaaaagaat gatgaggacc aaaagatcat ggtgagcaag
     2521 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2581 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2641 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2701 ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     2761 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2821 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2881 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2941 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3001 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3061 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     3121 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3181 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     3241 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3301 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3361 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3421 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaagcttat
     3481 aacttcgtat agtatacctt atacgaagtt attacgtaaa atggctccga aaactagtgg
     3541 aaaggcagcc aagaaggctg gcaaggctca gaagaacatc accaagaccg acaagaaaaa
     3601 gaagcgcaaa aggaaggaga gctatgccat ctacatttac aaggttctca agcaggtcca
     3661 tcctgacacc ggaatttcgt cgaaggcgat gagcataatg aacagctttg taaatgatat
     3721 tttcgagcga attgctgccg aagcgtctcg tctagctcac tacaacaagc gctcgaccat
     3781 caccagtcgg gagatccaaa cggctgttcg cctgcttttg cctggagagt tggccaagca
     3841 tgctgtcagt gagggaacca aggctgtcac caagtacacc agctctaaac tcgagatggt
     3901 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3961 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4021 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     4081 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     4141 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4201 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aagacggcga
     4261 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4321 gaagaagacg atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4381 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4441 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4501 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4561 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggatccg gagccacgaa
     4621 cttctctctg ttaaagcagg caggagatgt ggaagaaaac cccggccctg ccatggttaa
     4681 ttaacctgca ggctagatca taaactcatc ggcgcgccgt acgcgtatcg ataagcttta
     4741 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     4801 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     4861 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     4921 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga gtcgcggccg
     4981 ccttaacgaa ataattaatt gtttccattc gacacgatat tggtgctatt tgccgacccc
     5041 caattgcaca aatgacttct catagacgta gacaaggggg ggatgtagga ccgtggtatc
     5101 aaaaacgtcg ataaattgaa aaggtccacg aaaactttat accgatcgct gaaaaacaaa
     5161 atcaaacatt gcaaaaaccg tgttgaaatc aatttcacct taacgttgac cttaaatact
     5221 gataccccga agagccccct gttcctaact gcgaagaacc aatcattctt tgcaccatca
     5281 aaacatgatt atctgaaatt ataaaattta aacaaaaaca attgacgtac ggatttacta
     5341 acaccgccat ttcgtgatca gcgagtctat gtccatcaaa agtgttagaa tttggtgggg
     5401 ctgactgaac ctgggttcgt ccctgtaaaa ggtgatcatg atagcccctt gcagggcttt
     5461 gtgtgagaag cgcctctcgc cgcgcgacat tagagcctca tggacctcaa ctttaaaaat
     5521 cgactcgtaa acctcgcgtc tcttctccaa atcgttcaat ttgtaacgct ttaaagtctc
     5581 cgcctcctcc ttctccgcca gctctttttg ctcggttaaa agcttctcga ccgctttttg
     5641 gtacttcccc cagaaattaa acccctcgag ctccagcccc ggcgttcgct ccagccaccg
     5701 ctggaccaaa tcggtcaaag acggctcttt ctccgacttg gcgatttgct cgagcgcttt
     5761 ttcgtcattt ccgaacactt tcgagtagtt ctggttgtat ttgacgcggt tttcctggcg
     5821 caccccgagc ttgttttcca ggagcctgaa ctgcaggctc tggaacccgg aagcgggccg
     5881 cagataacac cggaagtcca tgaaatcgag cggtgtcatc gtttccagaa tcatcacttg
     5941 gtccaccaag acctacaatt gcgacagttt tatctcgtga ccgcaatttg ttgcgaaatt
     6001 gtgaaccttg aggatcaaaa caacacgatt gaggcgtttg aggatttcca aagtttgcga
     6061 ctcttctaaa acgtcgctga aaatgttgcg gatcgagtcc agctcgtaga ttatttgttt
     6121 gaaccataac tcatacgctg gaattacttg ttatagttaa ttacggttaa gtgggttttt
     6181 taccttgatg ggtcacaata aataaatgct cgtcgtgcac gggctggtta ttttgctcgc
     6241 tgagtaatct ttgggcttct aaaatcttgt ccaacatcaa gtattcaccg taaagcatcc
     6301 cacattcttc gctcagttgg tcgccttctt gggcttcact taatttacga ttaattgtaa
     6361 atggaataat taaattaaat catacttacg agggtctcag tgggcaactc attacgaccg
     6421 cggtaccgtc gacgttctag cggtaccccg attgtttagc ttgttcagct gcgcttgttt
     6481 atttgcttag ctttcgctta gcgacgtgtt cactttgctt gtttgaattg aattgtcgct
     6541 ccgtagacga agcgcctcta tttatactcc ggcggtcgag ggttcgaaat cgataagctt
     6601 ggatcctaat tgaattagct ctaattgaat tagtctctaa tgaataatga tccccgggcg
     6661 agctcgaatt aattcaacca ttgtgggaac cgtgcgatca aacaaacgcg agataccgga
     6721 agtactgaaa aacagtcgct ccaggccagt gggaacatcg atgttttgtt ttgacggacc
     6781 ccttactctc gtctcatata aaccgaagcc agctaagatg gtatacttat tatcatcttg
     6841 tgatgaggat gcttctatca acgaaagtac cggtaaaccg caaatggtta tgtattataa
     6901 tcaaactaaa ggcggagtgg acacgctaga ccaaatgtgt tctgtgatga cctgcagtag
     6961 gaagacgaat aggtggccta tggcattatt gtacggaatg ataaacattg cctgcataaa
     7021 ttcttttatt atatacagcc ataatgtcag tagcaaggga gaaaaggttc aaagtcgcaa
     7081 aaaatttatg agaaaccttt acatgagcct gacgtcatcg tttatgcgta agcgtttaga
     7141 agctcctact ttgaagagat atttgcgcga taatatctct aatattttgc caaatgaagt
     7201 gcctggtaca tcagatgaca gtactgaaga gccagtaatg aaaaaacgta cttactgtac
     7261 ttactgcccc tctaaaataa ggcgaaaggc aaatgcatcg tgcaaaaaat gcaaaaaagt
     7321 tatttgtcga gagcataata ttgatatgtg ccaaagttgt ttctgactga ctaataagta
     7381 taatttgttt ctattatgta taagttaagc taattactta ttttataata caacatgact
     7441 gtttttaaag tacaaaataa gtttattttt gtaaaagaga gaatgtttaa aagttttgtt
     7501 actttataga agaaattttg agtttttgtt tttttttaat aaataaataa acataaataa
     7561 attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact taatatctat
     7621 tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc aattttacgc
     7681 atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaaa taatagtttc
     7741 taattttttt attattcagc ctgctgtcgt gaataccgta tatctcaacg ctgtctgtga
     7801 gattgtcgta ttctagcctt tttagttttt cgctcatcga cttgatattg tccgacacat
     7861 tttcgtcgat ttgcgttttg atcaaagact tgagcagaga cacgttaatc aactgttcaa
     7921 attgatccat attaacgata tcaacccgat gcgtatatgg tgcgtaaaat atatttttta
     7981 accctcttat actttgcact ctgcgttaat acgcgttcgt gtacagacgt aatcatgttt
     8041 tcttttttgg ataaaactcc tactgagttt gacctcatat tagaccctca caagttgcaa
     8101 aacgtggcat tttttaccaa tgaagaattt aaagttattt taaaaaattt catcacagat
     8161 ttaaagaaga accaaaaatt aaattattta atcgaccagt taatcaacgt gtacacagag
     8221 cgcaaaaaac acgcagcccg acgtgttggc taaaattatt aaatcaactt gtgttatagt
     8281 cacgatttgc cgtccaacgt gttcctcaaa aagttgaaga ccaacaagtt tacggacact
     8341 agttaattat ttgattttgc cccacttcat tttgtgggat cacaattttg ttatatttta
     8401 aacaaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     8461 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     8521 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     8581 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     8641 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     8701 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     8761 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     8821 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8881 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8941 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9001 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9061 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9121 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9181 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9241 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9301 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9361 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9421 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9481 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9541 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9601 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9661 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9721 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9781 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9841 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9901 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9961 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    10201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
    10441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10741 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10801 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10861 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    10921 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    10981 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11041 aattcgagct cggtacccgg ggatcctcta gagtcgacgc tcgcgcgact tggtttgcca
    11101 ttctttagcg cgcgtcgcgt cacacagctt ggccacaatg tggtttttgt caaacgaaga
    11161 ttctatgacg tgtttaaagt ttaggtcgag ta
//
