LOCUS       sequence-226423-        7873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7873)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-FAK-5
FEATURES             Location/Qualifiers
     source          1..7873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4601..4722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4638..4657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4692..4711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4729..5184)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4866..4887)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5078..5097
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5211..5315
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5317..5674
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5317..5508
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5340..5360)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5525..5660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5709..6503
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5709..6503
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5763..5782)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6373..6392
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6691..6710)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6735..6782
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7111..7699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7600..7619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgcgcggcga gggcgagggc
      661 gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcgccacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatca gcttcaagga cgacggcacc tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat caccgccgac
     1021 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacgtgga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gtgctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag
     1261 ctgtacaagt ccggactcag atctatggca gcagcttacc ttgatccaaa cttgaatcat
     1321 acaccaagtt caagtgcaaa gacgcacctc ggtactggga tggagcgttc cccgggggcc
     1381 atggagcgag tcctaaaggt ttttcactac tttgaaaaca gcagcgagcc aacgacgtgg
     1441 gccagcatta tccggcatgg agatgctact gatgttcgag gcataataca gaagattgtg
     1501 gactgtcaca aagtgaaaaa tgtggcctgc tatgggttgc gactcagtca tctgcagtct
     1561 gaggaggttc actggctgca cctggacatg ggggtatcca atgtgagaga gaaatttgaa
     1621 ctagcacatc ctccagaaga atggaaatat gaactgagaa ttcggtacct gcccaaagga
     1681 tttctaaacc agttcactga ggacaaacca actttaaatt ttttctatca gcaggtgaaa
     1741 aatgactata tgttagaaat agcagatcaa gtggaccagg aaattgcttt gaaactaggt
     1801 tgccttgaaa tcaggagatc ctacggagag atgagaggca atgcattaga gaagaaatcc
     1861 aactatgaag tgctagaaaa agatgtcggt ttaagacgat tttttccgaa gagtttgcta
     1921 gattcagtga aggccaaaac actacgaaaa ttaatccaac agacatttcg acaatttgcc
     1981 aacctcaaca gagaagaaag tattttgaaa ttctttgaga tcctctctcc agtgtacaga
     2041 tttgacaagg aatgcttcaa gtgtgccctt ggttcaagct ggattatttc agtggagctg
     2101 gcaattggcc cagaggaagg aatcagctac cttacagaca agggtgcaaa tccaactcac
     2161 ctggcagatt ttaatcaagt acaaactatt cagtattcaa acagtgaaga caaggacaga
     2221 aaagggatgt tgcaactgaa gatagctggt gcacctgagc ctctgacagt gacagcacca
     2281 tccttaacca ttgcagagaa tatggctgac ttgatagacg gatactgccg actggtgaat
     2341 ggagccacgc aatcttttat tatcaggcca cagaaagaag gtgaaagagc tttaccatca
     2401 ataccaaagc tggccaacaa tgagaagcaa ggagtaaggt cgcacacagt ctctgtatca
     2461 gaaacagatg actatgcaga gataatagat gaagaagata cttatacaat gccatcaacc
     2521 agagattatg aaattcaaag ggagagaatt gaactggggc gctgcattgg tgaaggacag
     2581 tttggagatg tgcaccaagg aatttacatg agtccggaaa atccagctat ggctgtagca
     2641 atcaaaacat gtaaaaactg cacctcagac agcgttagag aaaagttcct acaagaagcc
     2701 ttaacaatgc gtcagtttga tcatcctcac attgtgaagc tcattggagt tattacagaa
     2761 aacccagtgt ggataatcat ggagctctgt acacttggag agttgagatc gtttctgcaa
     2821 gtaagaaaat tcagcttgga cctggcctcc ctcatcctct acgcttacca gcttagcaca
     2881 gcacttgctt acctagagag caaaagattt gtacatagag atattgctgc taggaacgtg
     2941 ctggtatctg ccactgactg tgtgaaattg ggtgactttg gcttatcccg atacatggaa
     3001 gacagtactt actataaagc ttccaaagga aagttaccta tcaaatggat ggctccagag
     3061 tcaatcaact tccgacggtt tacctcagca agcgatgtgt ggatgtttgg tgtgtgtatg
     3121 tgggagatcc tgatgcatgg ggtaaagccc ttccagggag tgaaaaataa tgatgttatt
     3181 ggtcggattg agaacggtga gcggctcccc atgcctccga actgccctcc caccctctac
     3241 agccttatga ccaagtgctg ggcatacgac cctagtagac gacccaggtt tactgaactt
     3301 aaagcacaac tcagtacaat actggaggag gagaagctgc agcaagagga acgaatgaga
     3361 atggaatcca ggcgacaagt cacagtatcc tgggactcag gaggatcaga tgaagctcct
     3421 cccaagccca gcaggcctgg ttaccccagc ccaaggtcca gtgaagggtt ttatccgagt
     3481 cctcagcata tggtacagcc aaatcactac caggtatctg gctactctgg ttctcatggg
     3541 ataccagcca tggcaggcag catttatcct gggcaagctt ctctcttgga tcaaacagat
     3601 tcctggaacc atcgacctca ggaagtatca gcatggcagc caaacatgga ggattcgggc
     3661 actttggatg tacgaggaat ggggcaggtt ctgcccacac atctcatgga ggagaggtta
     3721 ataagacaac agcaagagat ggaagaagat caacgctggc ttgagaaaga ggaacgattc
     3781 ctggtaatga aacctgatgt gcggctctcc agaggcagca ttgaacggga ggacggaggt
     3841 ctccagggcc cagctggtaa ccagcacata tatcagcctg tgggtaaacc agatcatgcc
     3901 gctccaccaa agaagccccc tcgccctgga gccccccact tgggcagcct cgcgagcctg
     3961 aacagccccg tggacagcta caacgaaggc gtgaagatca agccacagga aatcagccct
     4021 cctcctacgg ccaacctgga ccgctccaat gacaaagtct atgagaatgt aaccgggctg
     4081 gtgaaagctg tcatagagat gtccagtaaa atacagccag ctccgccaga ggagtacgtg
     4141 cccatggtaa aggaggttgg cttggcgctg agaaccttgc tagcaacagt ggatgagtcg
     4201 ctgccagtgc ttcctgcaag cacccacaga gagattgaga tggcccagaa actgctgaac
     4261 tctgacctgg ctgagctcat taacaagatg aagctggccc agcagtacgt catgaccagc
     4321 ctgcagcagg agtacaagaa gcaaatgctg acggctgctc acgctctggc tgtggatgcc
     4381 aagaacttgc tggatgtcat cgatcaagcc agactgaaaa tgatcagcca gtccaggccc
     4441 actaagctgc cgtagccgaa ttctgcagat gggatccacc ggatctagat aactgatcat
     4501 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4561 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4681 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     4741 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4801 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4861 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4921 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4981 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5041 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5101 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5161 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5281 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     5341 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5401 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5461 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5521 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5581 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5641 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     5701 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5761 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5821 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5881 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5941 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6001 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6061 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6121 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6181 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     6241 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6301 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6361 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6421 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6481 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6541 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6601 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6661 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     6721 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     6781 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     6841 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     6901 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     6961 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7021 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7441 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7501 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7561 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7621 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7681 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7741 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7801 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     7861 atatggagtt ccg
//
