LOCUS       sequence-226431-       10435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion
            protein. Used to package EnvA pseudotyped G-deleted Rabies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
  TITLE     Life-Long Genetic and Functional Access to Neural Circuits Using 
            Self-Inactivating Rabies Virus.
  JOURNAL   Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 
            10.1016/j.cell.2017.06.014.
  PUBMED    28689641
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014."
COMMENT     Sequence Label: pLenti-CMV-CAG-GFP-EnvA
FEATURES             Location/Qualifiers
     source          1..10435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1793..2096
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2097..2300
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          2449..2827
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             join(2935..2937,2938..3648)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2977..2998)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3238..3257)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3585..3606
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3658..3723
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    5608..6196
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5661..5681)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6079..6090)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6283..6516
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6588..6709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6625..6644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6679..6698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6749..6884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6904..6923)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6905..6923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6933..6950)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6933..6949)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6942..6964)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7091..7546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7178..7197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7388..7409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7572..7676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7677..7745,7746..8537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7895..7914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8708..9296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9197..9216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9450..9467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9584..9605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9620..9625,9626..9643,9644..9650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9658..9674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9663..9685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9682..9698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9682..9698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9717..9737
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9719..9737
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        9838..10217
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10218..10421
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgatca cgagactagc ctcgactcta gaaagacgcg tctagttatt
     1741 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     1801 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     1861 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     1921 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     1981 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2041 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     2101 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     2161 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2221 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     2281 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     2341 gctgttttga cctccataga agacaccggg accgatccag cctccgcgga ttcgaatccc
     2401 ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtgacgt aagtaccgcc
     2461 tatagagtct ataggcccac aaaaaatgct ttcttctttt aatatacttt tttgtttatc
     2521 ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat
     2581 gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg caatagcaat
     2641 atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg
     2701 ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg
     2761 gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct
     2821 cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg gcaaagaatt
     2881 gggattcgaa catcgattga attcggtacc cttcaggtcg actccggagc caccatgagc
     2941 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     3001 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     3061 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     3121 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     3181 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3241 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3301 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3361 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3421 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3481 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3541 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3601 ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaaggg atccggagtg
     3661 aaacagactt tgaattttga ccttctcaag ttggcgggag acgtggagtc caacccaggg
     3721 cccatggaag ccgtcataaa ggcatttctg actgggcacc ctggaaaggt gagcaagaag
     3781 gactccaaga agaagccgcc agcaacaagc aagaaagacc cggagaagac gcccttgctg
     3841 ccaacgagag ttaattatat tattggtgtc ctggtcttgt gtgaggtaac gggggtaaga
     3901 gctgatgtcc acttactcga gcagccaggg aacctttgga ttacatgggc caaccgtaca
     3961 ggccaaacgg atttttgcct ctctacacag tcagccacct ccccttttca aacatgtttg
     4021 ataggtatcc cgtcccctat ttccgagggt gattttaagg gatatgtttc tgatacaaat
     4081 tgcaccacct tgggaactga tcggttagtc tcgtcagccg actttactgg cggacctgac
     4141 aacagtacca ccctcactta tcggaaggtc tcatgcttgt tgttaaagct gaatgtctct
     4201 atgtgggatg agccacctga actacagctg ttaggttccc agtctctccc taacattact
     4261 aatattgctc agatttccgg tataaccggg ggatgcgtag gcttcagacc acaaggggtt
     4321 ccttggtatc taggttggtc tagacaggag gccacgcggt ttctccttag acacccctct
     4381 ttctctaaat ccacggaacc gtttacggtg gtgacagcgg ataggcacaa tctttttatg
     4441 gggagtgagt actgcggtgc atatggctac agattttgga acatgtataa ctgctcacag
     4501 gtggggcggc agtaccgctg tggtaatgcg cgcagccccc gcccgggtct tcctgaaatc
     4561 cagtgtacaa ggagaggagg caaatgggtt aatcaatcac aggaaattaa tgagtcggag
     4621 ccgttcagct ttacggtgaa ctgtacagct agtagtttgg gtaatgccag tgggtgttgc
     4681 ggaaaagcag gcacgattct cccgggaaag tgggtcgaca gcacacaagg tagtttcacc
     4741 aaaccaaaag cgctaccacc cgcaattttc ctcatttgtg gggatcgcgc atggcaagga
     4801 attcccagtc gtccggtagg gggcccctgc tatttaggca agcttaccat gttagcacct
     4861 aagcatacag atattctcaa ggtgcttgtc aattcatcgc ggacaggtat aagacgtaaa
     4921 cgaagcacct cacacctgga tgatacatgc tcagatgaag tgcagctttg gggtcctaca
     4981 gcaagaatct ttgcatctat cctagccccg ggggtagcag ctgcgcaagc cttaagagaa
     5041 attgagagac tagcctgttg gtccgttaaa caggctaact tgacaacatc actcctcggg
     5101 gacttattgg atgatgtcac gagtattcga cacgcggtcc tgcagaaccg agcggctatt
     5161 gacttcttgc tcctagctca cggccatggc tgtgaggacg ttgccggaat gtgctgtttc
     5221 aatttgagtg atcacagtga gtctatacag aagaagttcc agctaatgaa ggaacatgtc
     5281 aataagatcg gcgtggacag cgacccaatt ggaagttggc tgcgaggact attcggggga
     5341 ataggagaat gggccgttca tttgctgaaa ggactgcttt tggggcttgt agttattttg
     5401 ttgctagtag tgtgcctgcc ttgcagaaga gtgaacagaa gcgagcccac ccagcacaac
     5461 ctgagaggca ccggcagaga ggtgagcgtg accccccaga gcggcaagat catcagcagc
     5521 tgggagagcc acaagagcgg cggcgagacc agactgtgat aaaagcttgc ctcgaggatg
     5581 gggactgata aaagcttcga gctcgacaat caacctctgg attacaaaat ttgtgaaaga
     5641 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5701 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5761 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5821 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5881 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5941 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6001 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6061 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6121 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6181 tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg
     6241 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6301 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6361 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6421 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6481 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     6541 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6721 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6781 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6901 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6961 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7021 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7081 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7141 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7201 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7261 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7321 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7381 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7441 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7501 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7561 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7621 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7681 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7741 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7801 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7861 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7921 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7981 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8041 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8101 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8161 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8221 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8281 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8341 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8401 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8461 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8521 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8581 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8641 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8701 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8761 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8821 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8881 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8941 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9001 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9061 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9121 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9181 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9241 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9301 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9361 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9421 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9481 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9601 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9661 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9721 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt
     9781 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
     9841 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9901 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9961 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
    10021 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    10081 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
    10141 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
    10201 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
    10261 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
    10321 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
    10381 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccg
//
