LOCUS       sequence-226438-       16629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16629)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 16629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-167
FEATURES             Location/Qualifiers
     source          1..16629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      737..831
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             875..1534
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(941..960)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1599..1685
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1796..1961
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(2394..3344)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3392..3614
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     4297..4316
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            4354..4463
                     /note="incP origin of transfer"
     primer_bind     4486..4505
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4634..5425,5426..5494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5257..5276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5495..5586)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5605..5632)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5724..5810)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5963..5980
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12671..12955)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12729..12748)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12982..13860
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(14298..15497)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcgtggcc tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct
      301 ttcaaggacg acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacttcaa cagccactac gtctatatca cggccgacaa gcagaagaac
      481 tgcatcaagg ctaacttcaa gatccgccac aacgttgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagcc atcagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatt acacatggca tggacgagct gtacaagtaa
      721 taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      781 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag
      841 cttggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
      901 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
      961 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1021 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1081 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1141 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1201 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1261 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1321 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1381 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1441 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1501 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     1561 ttaaacgcct ggggtaatga ctctctagct tgaggcatca aataaaacga aaggctcagt
     1621 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1681 caaatccgcc gctaggagct tgcggccgcc aggggatctg attaattaaa acgtggctta
     1741 ctaggatccg atatcattta aatctaggga taacagggta atactagtgt cgaccaacca
     1801 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     1861 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     1921 tttccacccc tcccagttcc caactatttt gtccgcccac aggctgaaag cgctatttct
     1981 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
     2041 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
     2101 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
     2161 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
     2221 cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     2281 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
     2341 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     2401 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     2461 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
     2521 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
     2581 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     2641 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     2701 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     2761 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     2821 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     2881 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     2941 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     3001 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     3061 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     3121 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgaacg
     3181 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     3241 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     3301 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     3361 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
     3421 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     3481 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     3541 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     3601 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     3661 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     3721 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     3781 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     3841 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     3901 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     3961 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     4021 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     4081 ccgtcttact gtcggggatc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     4141 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     4201 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     4261 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     4321 agcaaccgca cctgtggcgc cggtgatgcc gccgggcagg ataggtgaag taggcccacc
     4381 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     4441 tcctgctctg cgaggctggc cggcggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4501 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4561 cgccgcgctt aatgcgccgc tacagggcgc gtaaatcaat ctaaagtata tatgagtaaa
     4621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4921 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     4981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5221 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     5281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5461 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5581 aacaaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt
     5641 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca
     5701 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     5761 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca
     5821 gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc
     5881 tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aattctgttt
     5941 tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc
     6001 gccaaaacag ccaagcttgc atgcctgcag gtcgactcta gaggatcccc taaaaataat
     6061 gaccgagcta gctctaactg tgggatgtga taacgagtct tgctatatgg tgtttcagct
     6121 tcgtaaataa atgaactctg cgctccatcc ttgttgcgta cggaagcaaa ggacgagaga
     6181 ttaggaaaat cgattatttt ggctcgttga agatccgatg cttcaaactc aatcacacga
     6241 tccgccttat ttatttgctt tgttgtactg ttaaccacct tagctcgact taataaaggc
     6301 aaatgagtaa atcgagtcca tttccgttct tgcattggat taaaccaaac aaaaatacgc
     6361 cactccttat gacccgagtt acggaataaa tgaccaatat gtacaacctg cacattgtca
     6421 ttaaatgtag ctagcctaac cactcactct tccccataaa ccatttccaa gaatctttgt
     6481 gcttcctcag ttatccgctc tttagataac gttgcacttc ttaataatcg ccagcgccta
     6541 agctcaacat cttcctgttt aagcttaata aaagcttggc taatccgtct aatttgataa
     6601 tcttccacac tctctgagta acgctttaag caaagcgcta ccaaaggcag tttctgtaga
     6661 ttttttgcaa gagaggttcc gttaggagtt tgctttaaca gccagctcga tgtcgctctt
     6721 gggtgatcaa ggctactatc tagacgcttt atgattctta atagttgtcg tacagcaatt
     6781 cgatctcttt ggttccaatc aactctaggg gcgggtgcca gacgctcttg ttgatgctgt
     6841 tgattccagt gaactagcca atccctgtca tgtcggtaaa gccaagcgta tagcacccca
     6901 ccctctaaag actgtcttgc cgccttaatt ccttggtatt tatgcactag ttgctgccag
     6961 tctttatgct taacagataa atcttcagag ttaggttgca cagactggct aacaggtctt
     7021 gtcgttatag agtgctcagt aagagcactt gcctgctgta gcgcttctat aaccgttagt
     7081 tttggcaata aggcttgcca cacaatacta tgctgtaaat aactaaaggc ttttctatgc
     7141 ttacggaata tacttttcag ccaacacgtg tccttgttct ctgccaactt taaatccagt
     7201 ttctctagtg cctcatcact aaaagtctgc ctcactctct ccgccaccaa gtcatgacga
     7261 atgtgcttgc ttttggttag ccctagatcc tgcgctaagc gctgataaaa cagcgtccac
     7321 tgctcaaggc ttggggaaag ctcttgcgct cgtggagcat ctaacagagg ggctatataa
     7381 gcagctagtg ctgttaattg agatagggag tctttggggt agtctgaaag cagctcagta
     7441 tgacccaaag cccaaaattg atgtcggtga tcatctacag ctctatcaaa gaagactaaa
     7501 gcaccgtgtt ttggacaata tggcaaagcg ggcaaatacc aatctcgttg ccaaaaggct
     7561 tccccatacc tatttagctg aagagcaacg caatcagggc agtatctaaa gcggttatcg
     7621 ctcttaactc tagaagcagc gactcctagc attaaatgca ccgcaccttg cgcttggtac
     7681 tccattaacc gaatagcttc gtctcggcgc tccttgccta caaacggagc atataaaggg
     7741 aataaggtat gctcataaat aagctgctga acagcgtaac gtcctgtttg atgtagatgt
     7801 cttgctatca cacctaaatg cgagggcaga cctaaggtag cgaccacctt gcggttgcca
     7861 tacacctcat ccaacagctg cttaggacta acaatccctt gataaacgcc tgcccgtgca
     7921 atagtgctat aaatcagctc attcgagtac ggaacaggaa agtttctcat ggctaacctg
     7981 cttgtttaaa taagctcgcc atatccacta ttaccccttt cccttttaac cgttcatgca
     8041 tggttttttc aggttggcgt tgtgaataga tataacgtaa atccgtatca ggcaagctat
     8101 cccagtcgct gggcttgact acctttatag ccgaaacctt tttactcttg gagggctttt
     8161 ctagttctga cactaccgtt tcgccttcca tcaaccactg caaaacaaga ggcagtaact
     8221 tttgtcttgt catcgttgga ttctggctaa acgctttttt aatagtggga attaacaggc
     8281 ttgaatcgta atcctctttc agcatcagat ataaatgacg ctgatcttcg gtatctaact
     8341 cttgaagggc tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct agctgaagtt
     8401 ggattaaccg tttatcaatc tcgggaacga ctagatcaga ataacgagca atgcgttctg
     8461 ggatacccga gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca tcttgataca
     8521 cttgccgcaa taaaccagcg gtaatacgct cattgcctaa agctagcgca cggagctgag
     8581 cgagtacaaa aagttttact acaatgtcca tcactccttg gcttagctca taccacacat
     8641 cacggacctc atccgataac agcgcatctt tgcgttgtaa aagctgtaat tgccaaagat
     8701 tatccgtaaa agcgatccac tcttgattgg gctttccacg ttgcgtttgt tgtataggat
     8761 cccagaatat agctccaaac cctgcccctc tacgtgcaga ccgcaaatca gcctcaaaaa
     8821 tctctcgtgc tttaggggta ccaatcaaca tcactggtac gccaataata ttcaccatcg
     8881 tcacaaaaaa gttcagcatc tcttgagatc cacccgaacg agagcggctt aaatgctgaa
     8941 tttcatcaat aaccaacaac cctaaagcat gtgcattggc tatttgcgac atcaaagcca
     9001 acatggtttc tataccatga cgttttaagc cataacgacg ctcatagttc gagcccaagg
     9061 ctcgatccaa cgctctgaaa aaattcaagc agatttcttt tagcgaacca ttatgcgagc
     9121 agtctatttt caaatacacc acctgctcta cattgagttc acgatggtaa atcacctgag
     9181 gatacgtggc tagaatacga tgaagagagg tcgtcttccc actaccagaa caaccaatta
     9241 acaataagct ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc aactctcccg
     9301 tttgaacacg ctcataacca ttttgtaaat gcttttgtaa atctcctgtt ttaggatttc
     9361 tgcctacgta gccacctcga atcatgaccg aaatacgctc acttagtagc aaatgcgtac
     9421 ctaatggctg aaaatagtca tctggaatac gacaaatggt atgagctctg ataacacggg
     9481 acttttgcaa gtcagaggaa gtaagctgta aagaggattt cagtgatgca gcactattca
     9541 ctgactcttg taatggtggt aaggcctcga taaaaggatt atcacgataa gcctctaccc
     9601 ccgtatcacg ataaactgct tgaatccggg tagcactcat gactcatcct tttctggtgg
     9661 atcctgaaat aattcaggca cgtatgtggg taggctgtaa tcttcttgat catccgcttc
     9721 cactgcgttg aaaggaataa ctttagcctc atcacctgaa gagcttggct tcaaatgctc
     9781 cgcacgtttt ttacgctccg aggtcacggc ttctttttta ttagttttaa tctgcttaat
     9841 gcgtgttgat ttgggctcag tagtactggg cgttaactta ttcgctttct gaatggtttg
     9901 ctgaataaac gcctcaagct ccctgcgttt agttaactca tcctgcttcg cattggcttt
     9961 attgtgtttt tcttgtgctt gtatatccca aacctcccaa aatgagagac ctttaaactg
    10021 ccgactacgt tccgtcagat tacagcgcca aaatacacgg ctgccaactt gcggaaacaa
    10081 ataaatcgta tcaaccagca ctgggtcata agccgcttct aaatgttgag gtctagctat
    10141 atcagtgctc cgctgcaacc aaccctcacg cagaatctct gaccccgagt aatacaaacc
    10201 ccacaaatta acgccaaatg aagaaataga gacctttcgg cgaggcagta acgctactcg
    10261 caactgctct tgctccacag cccttaaact acctgtacga tgctgcatac cccattgcca
    10321 tagctggaca ggaatagacg gtaaatctgt aggaaaatca gcatctcgat cgtatttatc
    10381 catcaccaga tggttatttc tgaataagat cgtacgcaaa ataatttgtg tgaactcaaa
    10441 taccgacaga gatgcatcta acctatagtc tgtttcacca tggcttttga tccgactgcc
    10501 ctctacaatg ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa aagtgctttc
    10561 cactatgcct ttagcatcgc cacgtctagg tggagcactt tccactcgca cattaaaact
    10621 agaaactaag gcttcgacct gatgactcat taattcgcca cggtccgcta gcaacacatc
    10681 tggcaaacct acacacggcc agtctgagct actaatctca atatcatgct gggcacaaat
    10741 ggccgttttg tcagagcaag catttacaaa agcctgcatc gccaccacat aagacggatt
    10801 ttcaaagccg atataaaagc ccgtgatcat ccgactaaac acatcaatca caatgtaaag
    10861 cgttggtctt cctatgattt tttggcgatc atgatgatcc actaaataaa tatcagcaat
    10921 cgtggcatca atctcataac gactcccagg gcctaacgcc tgagaagtgg ctgtactact
    10981 taagggtcgt acgtcttttt tatatacccc tgctttaact ctagacttta agcgctgagc
    11041 tttagggtat tctcgatcat aaaaataacg aaactgacgt agtgttgggt aatcctcttg
    11101 gggaatgcga ggaaaatact gagcaaacaa gtccacaaat cgtctatagg caacggtggt
    11161 ctttgtacct ttttgattta acaggtgctt ttctatggtc aacctaaaaa ggcgttcaat
    11221 ctcgggcgtt accttggttc cttcaccctt accatattct ctggctcggc caatctttgc
    11281 tgttcctgtc gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt caggaattaa
    11341 tgcattaggc gtttgaccac gctgccagta acggcgtaac aacttataaa ctgtagcctt
    11401 agtaacctta tgttcttgga ccacatgctc aacgagttcg cttctgactt tagggtcgaa
    11461 acgatcctta ctattaataa taggaagaat ttttcgataa tcctcctcgc gcttctggaa
    11521 gctgacagaa tctacagacg gctcttctaa atcaagatgt acataagggt catcagttct
    11581 aactaagcgg ccttcatcta aatactgcat tagcaacaac tcagccctag cttgtggaac
    11641 tcctttatca gcgcttattt gcatccagac aacttggcca tcctctatag ccaaaatgcg
    11701 atacggatca ttatcaaata gcacaacctc attaatttgc cacatagcgc aactcctcca
    11761 tattcactac ttggctaata cagagatctg cacacttatt tgccctgaaa gacttataaa
    11821 tattgaactt aataaaacca tttgcggtta aggctcgtat ctcactcaat gttttgccta
    11881 actccaaatc ataagcaata tcaacctgct tacagacatt gataatgttt tcatctcctt
    11941 tttcttgcag gatatgggcc aatggggata gttgtgctaa aagctccgca gaaacttctt
    12001 ctgttttcac tgaataaagc cattcaatat tttcttttac tacgggattt atttctttat
    12061 cagtaaaaat gaaccaagga atttgctttt gctgccaata gcgacgctct agttctagtt
    12121 tttctaaggt acgctcgtct tgtaaggctg ctgcaggttt gacttgaata gcaaactgct
    12181 caaaaggacc atctttgcag tccactaaaa aatcagtaga cataacctga tctacaccac
    12241 gaataacagg atgcttaata ccactatcta ttgcaatctg cctggtatca ctaggtaata
    12301 aggggaactg ctcgcgtata tctagcacgc tgctctccca ctcaagactg agaaaaacag
    12361 caagctctaa gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa taaatacggt
    12421 gggaacgacc tgaagaagga acttcttgta ctgttagcca tggaatatag tctttaccat
    12481 gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt
    12541 ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca
    12601 gtttacaact attctgtcta gtttaagact tgggtaccga gctcgaattc gctagcccaa
    12661 aaaaacgggt atggagaaac agtagagagt tgcgataaaa agcgtcaggt aggatccgct
    12721 aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg
    12781 tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc
    12841 ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc
    12901 cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttctctg
    12961 aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct gccgggatac
    13021 tcgtttaatg cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat
    13081 ttttttatcg accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt
    13141 cagggggtgg tgaaaaatca gggacgagaa tttgtttgcc gaccgggtga tattttgctg
    13201 ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac
    13261 cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata
    13321 tttgccaata cggggttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg
    13381 tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata
    13441 aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca
    13501 ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc
    13561 aattttgata tcgccagcgt cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat
    13621 cttttccgcc agcagttagg gattagcgtc ttaagctggc gcgaggacca acgtatcagc
    13681 caggcgaagc tgcttttgag caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt
    13741 ggttttgacg atcaactcta tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg
    13801 agcgagttcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa
    13861 ttggtaacga atcagacaat tgacggcttg acggagtagc atagggtttg cagaatccct
    13921 gcttcgtcca tttgacaggc acattatgca tcgatgataa gctgtcaaac atgagcagat
    13981 cctctacgcc ggacgcatcg tggcctgtgg gcggacaata aagtcttaaa ctgaacaaaa
    14041 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca
    14101 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca
    14161 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggggtcga cgcggccgtg
    14221 gcgcgcctcc taggtgctcg agtggcggcc gctattgacc cataaactgc caggaattgg
    14281 ggatcggatt cgagctctca gcgatcggct cgttgccctg cgccgctcca aagcccgcga
    14341 cgcagcgccg gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc
    14401 cacgttgtta tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc
    14461 aggctggtga gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc
    14521 ctggacaaca ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg
    14581 atcgggaacg ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg
    14641 gcaatcattc cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg
    14701 atcattgcct gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg
    14761 gtcgcgtccc agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt
    14821 ccgacaagtt gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg
    14881 gcccaccgga acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct
    14941 ttgtgcgact ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc
    15001 gcggcgaaga acggagcgtg gggggagaaa ccgcccatca gcccaccgag cacaggtccc
    15061 gcgaccatcc cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg
    15121 ccatcagtga tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg
    15181 gccacgatcc gcccgatata gagaacccaa aggaaaggcg ctgtcgccat gatggcgtag
    15241 tcgacagtgg cgccggccag cgagacgagc aagattggcc gccgcccgaa acgatccgac
    15301 agcgcgccca gcacaggtgc gcaggcaaat tgcaccaacg catacagcgc cagcagaatg
    15361 ccatagtggg cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc
    15421 ggcataatca ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg
    15481 ggtatgttgg gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc
    15541 ggattcttta tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa
    15601 gcatgacaaa gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg
    15661 tcggcgtaga cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg
    15721 cgctttactg gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc
    15781 tggcggagaa tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga
    15841 tcgggaatgc ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc
    15901 atgccggcac gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc
    15961 tctgcgaggc gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact
    16021 tcactgttgg ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg
    16081 gcaccgttga acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg
    16141 aagccggtcc ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga
    16201 aaaggaggct cgttgtcagg aacgttgaag gaccgagaaa gggtgctaag aaaccattat
    16261 tatcatgaag ctcgcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
    16321 gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
    16381 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
    16441 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
    16501 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
    16561 gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat actagtggag
    16621 gaagaaaaa
//
