LOCUS       sequence-226439-       16644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16644)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 16644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-166
FEATURES             Location/Qualifiers
     source          1..16644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..735
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     terminator      752..846
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             890..1549
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(956..975)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1614..1700
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1811..1976
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(2409..3359)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3407..3629
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     4312..4331
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            4369..4478
                     /note="incP origin of transfer"
     primer_bind     4501..4520
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4649..5440,5441..5509))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5272..5291
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5510..5601)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5620..5647)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5739..5825)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5978..5995
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12686..12970)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12744..12763)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12997..13875
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(14313..15512)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 atggtgagca agggcgagga gctgatcaag gagaacatgc acatgaagct gtacatggaa
       61 ggcaccgtga acaaccacca cttcaagtgc accaccgaag gggagggcaa gccctacgag
      121 ggcacccaga cccagaggat taaggtggtg gagggaggcc ccctgccgtt cgcattcgac
      181 atcctggcca cctgctttat gtacgggagc aagaccttca tcaaccacac ccagggcatc
      241 cccgatttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      301 gaagacgggg gcgtgcttac cgttacccag gacaccagcc tccaggacgg ctgcttgatc
      361 tacaacgtca agctcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      421 acactcggct gggaggccac caccgagacc ctgtaccccg ctgacggcgg cctggaaggc
      481 agatgcgaca tggccctgaa gctcgtgggc gggggccacc tgcactgcaa cctgaagacc
      541 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac
      601 cgcagactgg aaagaatcaa ggaggccgac aatgagacct acgtcgagca gcacgaggtg
      661 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa tggcatggac
      721 gagctgtaca agtaataatt agctgagctt ggactcctgt tgatagatcc agtaatgacc
      781 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
      841 gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      901 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      961 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     1021 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     1081 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat
     1141 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1201 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1261 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1321 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1381 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1441 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     1501 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     1561 cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg catcaaataa
     1621 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1681 ctctcctgag taggacaaat ccgccgctag gagcttgcgg ccgccagggg atctgattaa
     1741 ttaaaacgtg gcttactagg atccgatatc atttaaatct agggataaca gggtaatact
     1801 agtgtcgacc aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     1861 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     1921 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacaggct
     1981 gaaagcgcta tttcttccag aattgccatg attttttccc cacgggaggc gtcactggct
     2041 cccgtgttgt cggcagcttt gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt
     2101 gtgactgttg agctgtaaca agttgtctca ggtgttcaat ttcatgttct agttgctttg
     2161 ttttactggt ttcacctgtt ctattaggtg ttacatgctg ttcatctgtt acattgtcga
     2221 tctgttcatg gtgaacagct ttaaatgcac caaaaactcg taaaagctct gatgtatcta
     2281 tcttttttac accgttttca tctgtgcata tggacagttt tccctttgat atgtaacggt
     2341 gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat acaagagcca
     2401 taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt tcgttgtttt
     2461 tgcgtgagcc atgagaacga accattgaga tcatacttac tttgcatgtc actcaaaaat
     2521 tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag tgtttttctt
     2581 agtccgttat gtaggtagga atctgatgta atggttgttg gtattttgtc accattcatt
     2641 tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag ttcaacttgg
     2701 aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat attgctgtaa
     2761 gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt aaactcatgg
     2821 tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc tatatttgcc
     2881 ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct catagagtat
     2941 ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt taactggaaa
     3001 agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga gaacttggca
     3061 tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag gattcctgat ttccacagtt
     3121 ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg
     3181 ttcatcatct gaacgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg
     3241 ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc
     3301 gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa ttcagacata
     3361 catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc tagtcaatga
     3421 taattactag tccttttcct ttgagttgtg ggtatctgta aattctgcta gacctttgct
     3481 ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt gtgtgttttt
     3541 tttgtttata ttcaagtggt tataatttat agaataaaga aagaataaaa aaagataaaa
     3601 agaatagatc ccagccctgt gtataactca ctactttagt cagttccgca gtattacaaa
     3661 aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc taaaggctta
     3721 agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc cttttgtctc cgaccatcag
     3781 gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt
     3841 gaatgggggt aaatggcact acaggcgcct tttatggatt catgcaagga aactacccat
     3901 aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc tgctatgtgg
     3961 tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag tctgaccact
     4021 tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag gcagcggtat
     4081 catcaacagg cttacccgtc ttactgtcgg ggatcgacgc tctcccttat gcgactcctg
     4141 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     4201 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     4261 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     4321 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccgccgg gcaggatagg
     4381 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     4441 ggtgctcaac gggaatcctg ctctgcgagg ctggccggcg gcgaacgtgg cgagaaagga
     4501 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4561 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtaaa tcaatctaaa
     4621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     4981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     5281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5581 gtatttagaa aaataaacaa aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     5641 gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     5701 ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     5761 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     5821 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
     5881 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc
     5941 aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa
     6001 aatcttctct catccgccaa aacagccaag cttgcatgcc tgcaggtcga ctctagagga
     6061 tcccctaaaa ataatgaccg agctagctct aactgtggga tgtgataacg agtcttgcta
     6121 tatggtgttt cagcttcgta aataaatgaa ctctgcgctc catccttgtt gcgtacggaa
     6181 gcaaaggacg agagattagg aaaatcgatt attttggctc gttgaagatc cgatgcttca
     6241 aactcaatca cacgatccgc cttatttatt tgctttgttg tactgttaac caccttagct
     6301 cgacttaata aaggcaaatg agtaaatcga gtccatttcc gttcttgcat tggattaaac
     6361 caaacaaaaa tacgccactc cttatgaccc gagttacgga ataaatgacc aatatgtaca
     6421 acctgcacat tgtcattaaa tgtagctagc ctaaccactc actcttcccc ataaaccatt
     6481 tccaagaatc tttgtgcttc ctcagttatc cgctctttag ataacgttgc acttcttaat
     6541 aatcgccagc gcctaagctc aacatcttcc tgtttaagct taataaaagc ttggctaatc
     6601 cgtctaattt gataatcttc cacactctct gagtaacgct ttaagcaaag cgctaccaaa
     6661 ggcagtttct gtagattttt tgcaagagag gttccgttag gagtttgctt taacagccag
     6721 ctcgatgtcg ctcttgggtg atcaaggcta ctatctagac gctttatgat tcttaatagt
     6781 tgtcgtacag caattcgatc tctttggttc caatcaactc taggggcggg tgccagacgc
     6841 tcttgttgat gctgttgatt ccagtgaact agccaatccc tgtcatgtcg gtaaagccaa
     6901 gcgtatagca ccccaccctc taaagactgt cttgccgcct taattccttg gtatttatgc
     6961 actagttgct gccagtcttt atgcttaaca gataaatctt cagagttagg ttgcacagac
     7021 tggctaacag gtcttgtcgt tatagagtgc tcagtaagag cacttgcctg ctgtagcgct
     7081 tctataaccg ttagttttgg caataaggct tgccacacaa tactatgctg taaataacta
     7141 aaggcttttc tatgcttacg gaatatactt ttcagccaac acgtgtcctt gttctctgcc
     7201 aactttaaat ccagtttctc tagtgcctca tcactaaaag tctgcctcac tctctccgcc
     7261 accaagtcat gacgaatgtg cttgcttttg gttagcccta gatcctgcgc taagcgctga
     7321 taaaacagcg tccactgctc aaggcttggg gaaagctctt gcgctcgtgg agcatctaac
     7381 agaggggcta tataagcagc tagtgctgtt aattgagata gggagtcttt ggggtagtct
     7441 gaaagcagct cagtatgacc caaagcccaa aattgatgtc ggtgatcatc tacagctcta
     7501 tcaaagaaga ctaaagcacc gtgttttgga caatatggca aagcgggcaa ataccaatct
     7561 cgttgccaaa aggcttcccc atacctattt agctgaagag caacgcaatc agggcagtat
     7621 ctaaagcggt tatcgctctt aactctagaa gcagcgactc ctagcattaa atgcaccgca
     7681 ccttgcgctt ggtactccat taaccgaata gcttcgtctc ggcgctcctt gcctacaaac
     7741 ggagcatata aagggaataa ggtatgctca taaataagct gctgaacagc gtaacgtcct
     7801 gtttgatgta gatgtcttgc tatcacacct aaatgcgagg gcagacctaa ggtagcgacc
     7861 accttgcggt tgccatacac ctcatccaac agctgcttag gactaacaat cccttgataa
     7921 acgcctgccc gtgcaatagt gctataaatc agctcattcg agtacggaac aggaaagttt
     7981 ctcatggcta acctgcttgt ttaaataagc tcgccatatc cactattacc cctttccctt
     8041 ttaaccgttc atgcatggtt ttttcaggtt ggcgttgtga atagatataa cgtaaatccg
     8101 tatcaggcaa gctatcccag tcgctgggct tgactacctt tatagccgaa acctttttac
     8161 tcttggaggg cttttctagt tctgacacta ccgtttcgcc ttccatcaac cactgcaaaa
     8221 caagaggcag taacttttgt cttgtcatcg ttggattctg gctaaacgct tttttaatag
     8281 tgggaattaa caggcttgaa tcgtaatcct ctttcagcat cagatataaa tgacgctgat
     8341 cttcggtatc taactcttga agggcttttt cttctggtgt ttgttcttgt atcgctgcga
     8401 tatctagctg aagttggatt aaccgtttat caatctcggg aacgactaga tcagaataac
     8461 gagcaatgcg ttctgggata cccgagcgta atgcctctag catggggtgc acaggcttta
     8521 actcatcttg atacacttgc cgcaataaac cagcggtaat acgctcattg cctaaagcta
     8581 gcgcacggag ctgagcgagt acaaaaagtt ttactacaat gtccatcact ccttggctta
     8641 gctcatacca cacatcacgg acctcatccg ataacagcgc atctttgcgt tgtaaaagct
     8701 gtaattgcca aagattatcc gtaaaagcga tccactcttg attgggcttt ccacgttgcg
     8761 tttgttgtat aggatcccag aatatagctc caaaccctgc ccctctacgt gcagaccgca
     8821 aatcagcctc aaaaatctct cgtgctttag gggtaccaat caacatcact ggtacgccaa
     8881 taatattcac catcgtcaca aaaaagttca gcatctcttg agatccaccc gaacgagagc
     8941 ggcttaaatg ctgaatttca tcaataacca acaaccctaa agcatgtgca ttggctattt
     9001 gcgacatcaa agccaacatg gtttctatac catgacgttt taagccataa cgacgctcat
     9061 agttcgagcc caaggctcga tccaacgctc tgaaaaaatt caagcagatt tcttttagcg
     9121 aaccattatg cgagcagtct attttcaaat acaccacctg ctctacattg agttcacgat
     9181 ggtaaatcac ctgaggatac gtggctagaa tacgatgaag agaggtcgtc ttcccactac
     9241 cagaacaacc aattaacaat aagctttgtg ccgtagatcg tgcctcctca aagcgaaatg
     9301 tctccaactc tcccgtttga acacgctcat aaccattttg taaatgcttt tgtaaatctc
     9361 ctgttttagg atttctgcct acgtagccac ctcgaatcat gaccgaaata cgctcactta
     9421 gtagcaaatg cgtacctaat ggctgaaaat agtcatctgg aatacgacaa atggtatgag
     9481 ctctgataac acgggacttt tgcaagtcag aggaagtaag ctgtaaagag gatttcagtg
     9541 atgcagcact attcactgac tcttgtaatg gtggtaaggc ctcgataaaa ggattatcac
     9601 gataagcctc tacccccgta tcacgataaa ctgcttgaat ccgggtagca ctcatgactc
     9661 atccttttct ggtggatcct gaaataattc aggcacgtat gtgggtaggc tgtaatcttc
     9721 ttgatcatcc gcttccactg cgttgaaagg aataacttta gcctcatcac ctgaagagct
     9781 tggcttcaaa tgctccgcac gttttttacg ctccgaggtc acggcttctt ttttattagt
     9841 tttaatctgc ttaatgcgtg ttgatttggg ctcagtagta ctgggcgtta acttattcgc
     9901 tttctgaatg gtttgctgaa taaacgcctc aagctccctg cgtttagtta actcatcctg
     9961 cttcgcattg gctttattgt gtttttcttg tgcttgtata tcccaaacct cccaaaatga
    10021 gagaccttta aactgccgac tacgttccgt cagattacag cgccaaaata cacggctgcc
    10081 aacttgcgga aacaaataaa tcgtatcaac cagcactggg tcataagccg cttctaaatg
    10141 ttgaggtcta gctatatcag tgctccgctg caaccaaccc tcacgcagaa tctctgaccc
    10201 cgagtaatac aaaccccaca aattaacgcc aaatgaagaa atagagacct ttcggcgagg
    10261 cagtaacgct actcgcaact gctcttgctc cacagccctt aaactacctg tacgatgctg
    10321 cataccccat tgccatagct ggacaggaat agacggtaaa tctgtaggaa aatcagcatc
    10381 tcgatcgtat ttatccatca ccagatggtt atttctgaat aagatcgtac gcaaaataat
    10441 ttgtgtgaac tcaaataccg acagagatgc atctaaccta tagtctgttt caccatggct
    10501 tttgatccga ctgccctcta caatgccagg tgcaaaggac ttaaactcgg cttgtagtgt
    10561 tctaaaagtg ctttccacta tgcctttagc atcgccacgt ctaggtggag cactttccac
    10621 tcgcacatta aaactagaaa ctaaggcttc gacctgatga ctcattaatt cgccacggtc
    10681 cgctagcaac acatctggca aacctacaca cggccagtct gagctactaa tctcaatatc
    10741 atgctgggca caaatggccg ttttgtcaga gcaagcattt acaaaagcct gcatcgccac
    10801 cacataagac ggattttcaa agccgatata aaagcccgtg atcatccgac taaacacatc
    10861 aatcacaatg taaagcgttg gtcttcctat gattttttgg cgatcatgat gatccactaa
    10921 ataaatatca gcaatcgtgg catcaatctc ataacgactc ccagggccta acgcctgaga
    10981 agtggctgta ctacttaagg gtcgtacgtc ttttttatat acccctgctt taactctaga
    11041 ctttaagcgc tgagctttag ggtattctcg atcataaaaa taacgaaact gacgtagtgt
    11101 tgggtaatcc tcttggggaa tgcgaggaaa atactgagca aacaagtcca caaatcgtct
    11161 ataggcaacg gtggtctttg tacctttttg atttaacagg tgcttttcta tggtcaacct
    11221 aaaaaggcgt tcaatctcgg gcgttacctt ggttccttca cccttaccat attctctggc
    11281 tcggccaatc tttgctgttc ctgtcgctga acgtctttcc cctggtgcac cgctgttttt
    11341 gtagtcagga attaatgcat taggcgtttg accacgctgc cagtaacggc gtaacaactt
    11401 ataaactgta gccttagtaa ccttatgttc ttggaccaca tgctcaacga gttcgcttct
    11461 gactttaggg tcgaaacgat ccttactatt aataatagga agaatttttc gataatcctc
    11521 ctcgcgcttc tggaagctga cagaatctac agacggctct tctaaatcaa gatgtacata
    11581 agggtcatca gttctaacta agcggccttc atctaaatac tgcattagca acaactcagc
    11641 cctagcttgt ggaactcctt tatcagcgct tatttgcatc cagacaactt ggccatcctc
    11701 tatagccaaa atgcgatacg gatcattatc aaatagcaca acctcattaa tttgccacat
    11761 agcgcaactc ctccatattc actacttggc taatacagag atctgcacac ttatttgccc
    11821 tgaaagactt ataaatattg aacttaataa aaccatttgc ggttaaggct cgtatctcac
    11881 tcaatgtttt gcctaactcc aaatcataag caatatcaac ctgcttacag acattgataa
    11941 tgttttcatc tcctttttct tgcaggatat gggccaatgg ggatagttgt gctaaaagct
    12001 ccgcagaaac ttcttctgtt ttcactgaat aaagccattc aatattttct tttactacgg
    12061 gatttatttc tttatcagta aaaatgaacc aaggaatttg cttttgctgc caatagcgac
    12121 gctctagttc tagtttttct aaggtacgct cgtcttgtaa ggctgctgca ggtttgactt
    12181 gaatagcaaa ctgctcaaaa ggaccatctt tgcagtccac taaaaaatca gtagacataa
    12241 cctgatctac accacgaata acaggatgct taataccact atctattgca atctgcctgg
    12301 tatcactagg taataagggg aactgctcgc gtatatctag cacgctgctc tcccactcaa
    12361 gactgagaaa aacagcaagc tctaagtcag atagcaaatg atggactcgt cccgtcttat
    12421 gagaataaat acggtgggaa cgacctgaag aaggaacttc ttgtactgtt agccatggaa
    12481 tatagtcttt accatgccct tggccacgcc cctctttaat acgacgggca atttgcactt
    12541 cagaaaatga agagtttgct ttagccataa caaaagtcca gtatgctttt tcacagcata
    12601 actggactga tttcagttta caactattct gtctagttta agacttgggt accgagctcg
    12661 aattcgctag cccaaaaaaa cgggtatgga gaaacagtag agagttgcga taaaaagcgt
    12721 caggtaggat ccgctaatct tatggataaa aatgctatgg catagcaaag tgtgacgccg
    12781 tgcaaataat caatgtggac ttttctgccg tgattataga cacttttgtt acgcgttttt
    12841 gtcatggctt tggtcccgct ttgttacaga atgcttttaa taagcggggt taccggtttg
    12901 gttagcgaga agagccagta aaagacgcag tgacggcaat gtctgatgca atatggacaa
    12961 ttggtttctt ctctgaatgg cgggagtatg aaaagtatgg ctgaagcgca aaatgatccc
    13021 ctgctgccgg gatactcgtt taatgcccat ctggtggcgg gtttaacgcc gattgaggcc
    13081 aacggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta tattctcaat
    13141 ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt ttgccgaccg
    13201 ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca tccggaggct
    13261 cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca tgaatggctt
    13321 aactggccgt caatatttgc caatacgggg ttctttcgcc cggatgaagc gcaccagccg
    13381 catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg gcgctattcg
    13441 gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga agcgattaac
    13501 gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta catcagcgat
    13561 cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg cttgtcgccg
    13621 tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag ctggcgcgag
    13681 gaccaacgta tcagccaggc gaagctgctt ttgagcacca cccggatgcc tatcgccacc
    13741 gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgggtatt taaaaaatgc
    13801 accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa tgatgtagcc
    13861 gtcaagttgt cataattggt aacgaatcag acaattgacg gcttgacgga gtagcatagg
    13921 gtttgcagaa tccctgcttc gtccatttga caggcacatt atgcatcgat gataagctgt
    13981 caaacatgag cagatcctct acgccggacg catcgtggcc tgtgggcgga caataaagtc
    14041 ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt
    14101 tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct
    14161 aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggg
    14221 gtcgacgcgg ccgtggcgcg cctcctaggt gctcgagtgg cggccgctat tgacccataa
    14281 actgccagga attggggatc ggattcgagc tctcagcgat cggctcgttg ccctgcgccg
    14341 ctccaaagcc cgcgacgcag cgccggcagg cagagcaagt agagggcagc gcctgcaatc
    14401 catgcccacc cgttccacgt tgttatagaa gccgcataga tcgccgtgaa gaggaggggt
    14461 ccgacgatcg aggtcaggct ggtgagcgcc gccagtgagc cttgcagctg cccctgacgt
    14521 tcctcatcca cctgcctgga caacattgct tgcagcgccg gcattccgat gccacccgaa
    14581 gcaagcagga ccatgatcgg gaacgccatc catccccgtg tcgcgaaggc aagcaggatg
    14641 tagcctgtgc cgtcggcaat cattccgagc atgagtgccc gcctttcgcc gagccgggcg
    14701 gctacagggc cggtgatcat tgcctgggcg agtgaatgca gaatgccaaa tgcggcaagc
    14761 gaaatgccga tcgtggtcgc gtcccagtga aagcgatcct cgccgaaaat gacccaaagc
    14821 gcggccggca cctgtccgac aagttgcatg atgaagaaga ccgccatcag ggcggcgacg
    14881 acggtcatgc cccgggccca ccggaacgaa gcgagcgggt tgagagcctc ccggcgtaac
    14941 ggccggcgtt cgcctttgtg cgactccggc aaaaggaaac agcccgtcag gaaattgagg
    15001 ccgttcaagg ctgccgcggc gaagaacgga gcgtgggggg agaaaccgcc catcagccca
    15061 ccgagcacag gtcccgcgac catcccgaac ccgaaacagg cgctcatgaa gccgaagtgc
    15121 cgcgcgcgct catcgccatc agtgatatcg gcaatataag cgccggctac cgccccagtc
    15181 gccccggtga tgccggccac gatccgcccg atatagagaa cccaaaggaa aggcgctgtc
    15241 gccatgatgg cgtagtcgac agtggcgccg gccagcgaga cgagcaagat tggccgccgc
    15301 ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac
    15361 agcgccagca gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg
    15421 aggcccggca gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc
    15481 agaattacga tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg
    15541 tccgattgaa cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca
    15601 gtgataaagt gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg
    15661 acctgttgaa cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg
    15721 gggttcagca gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac
    15781 tggccgaagc catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact
    15841 ggcgctcatt tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg
    15901 atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg
    15961 cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga
    16021 tgacaatcag ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg
    16081 ccggcgagcg cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga
    16141 tagacgcctt cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg
    16201 tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg
    16261 ctaagaaacc attattatca tgaagctcgc acgctgccgc aagcactcag ggcgcaaggg
    16321 ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
    16381 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
    16441 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
    16501 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
    16561 gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac
    16621 aggatactag tggaggaaga aaaa
//
