LOCUS       sequence-226442-        4614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ARL16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  TITLE     Kahn expression plasmids
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CS46
FEATURES             Location/Qualifiers
     source          1..4614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..107
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        88..106
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             138..160
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(713..731)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        799..903
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(904..972,973..1764)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1122..1141)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1935..2523
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2424..2443
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2677..2694
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2709..2848
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2774..2793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2934..2956
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2950..3141)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     join(4599..4614,1..4)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
       61 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
      121 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg tgtctcctgc
      181 ttggagctgc tggggtcggg aagaccctgc tggtgaaacg ccttcagaag ctgagctccg
      241 gagatggcaa aggcgacctg ggagagcccc caccgacgcg gcccacggtg ggcaccaacc
      301 ttacagacat tgtggcccac aggaagatca ccatccgaga gctcggggga tgcatgagcc
      361 ccatctggtc cagttactat ggaaactgcc attcgctctt gttcatgatg gatgcttcta
      421 accccaccca gctctctgca tcctgcatgc agctcctggg tctcctttct gcagaagaac
      481 ttgaaaaagc ctccgtcctc atactcttca ataaaattga cctaccctgt tacatgacca
      541 tggaggaaat gaagtcattg atgaggcttc cagacatcat tgcttgtgcc aagcaaaaca
      601 tcaccgcagt agaaataagt gccaggaatg gcactggctt ggcaacagta ttgctctggc
      661 tccagaatca acacaggcac agcagttgat aagaattctt gaagacgaaa gggcctcgtg
      721 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      781 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      901 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      961 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1021 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1081 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1141 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1201 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1261 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1321 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1381 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1441 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1501 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1561 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1621 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1681 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1741 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1801 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1861 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1921 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1981 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2041 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2101 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2161 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2221 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2281 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2341 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2401 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2461 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2521 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2581 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2641 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2701 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
     2761 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     2821 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     2881 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2941 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     3001 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     3061 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     3121 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     3181 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     3241 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     3301 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     3361 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     3421 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     3481 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     3541 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     3601 cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc
     3661 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     3721 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
     3781 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     3841 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     3901 gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
     3961 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     4021 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     4081 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
     4141 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     4201 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     4261 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
     4321 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     4381 aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag
     4441 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     4501 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     4561 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gata
//
