LOCUS       sequence-226443-        5886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5886)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..5886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1233
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
     polyA_signal    2614..2735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2651..2670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2705..2724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2742..3197)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2879..2900)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3091..3110
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3224..3328
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3330..3687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3330..3521
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3353..3373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3538..3673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3722..4516
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3722..4516
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3776..3795)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4386..4405
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4704..4723)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4748..4795
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5124..5712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5613..5632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgaacag cctgatcaaa gaaaacatgc ggatgaaggt ggtgctggaa
      601 ggcagcgtga acggccacca gttcaagtgc accggcgagg gcgagggcaa cccctacatg
      661 ggcacccaga ccatgcggat caaagtgatc gagggcggac ctctgccctt cgccttcgac
      721 atcctggcca catccttcat gtacggcagc cggaccttca tcaagtaccc caagggcatc
      781 cccgatttct tcaagcagag cttccccgag ggcttcacct gggagagagt gaccagatac
      841 gaggacggcg gcgtgatcac cgtgatgcag gacaccagcc tggaagatgg ctgcctggtg
      901 taccatgccc aggtcagggg cgtgaatttt cccagcaacg gcgccgtgat gcagaagaaa
      961 accaagggct gggagcccaa caccgagatg atgtaccccg ctgacggcgg actgagaggc
     1021 tacacccaca tggccctgaa ggtggacggc ggagggcacc tgagctgcag cttcgtgacc
     1081 acctaccgat ccaagaaaac cgtgggcaac atcaagatgc ccggcatcca cgccgtggac
     1141 caccggctgg aaaggctgga agagtccgac aacgagatgt tcgtggtgca gcgggagcac
     1201 gccgtggcca agttcgccgg cctgggcgga gggtccggac tcagatctgg tggctccgca
     1261 ggatcccggc aagaagaagc aaaggctgct gtaggtgtga ctggaaatga catcactacc
     1321 ccgccaaaca aggagccacc accaagccca gaaaagaaag caaagccttt ggccaccact
     1381 caacctgcaa agacttcaac atcgaaagcc aaaacacagc ccacttctct ccctaagcaa
     1441 ccagctccca ccacctctgg tgggttgaat aaaaaaccca tgagcctcgc ctcaggctca
     1501 gtgccagctg ccccacacaa acgccctgct gctgccactg ctactgccag gccttccacc
     1561 ctacctgcca gagacgtgaa gccaaagcca attacagaag ctaaggttgc cgaaaagcgg
     1621 acctctccat ccaagccttc atctgcccca gccctcaaac ctggacctaa aaccacccca
     1681 accgtttcaa aagccacatc tccctcaact cttgtttcca ctggaccaag tagtagaagt
     1741 ccagctacaa ctctgcctaa gaggccaacc agcatcaaga ctgaggggaa acctgctgat
     1801 gtcaaaagga tgactgctaa gtctgcctca gctgacttga gtcgctcaaa gaccacctct
     1861 gccagttctg tgaagagaaa caccactccc actggggcag cacccccagc agggatgact
     1921 tccactcgag tcaagcccat gtctgcacct agccgctctt ctggggctct ttctgtggac
     1981 aagaagccca cttccactaa gcctagctcc tctgctccca gggtgagccg cctggccaca
     2041 actgtttctg cccctgacct gaagagtgtt cgctccaagg tcggctctac agaaaacatc
     2101 aaacaccagc ctggaggagg ccgggccaag gtagagaaaa aaacagaggc agctaccaca
     2161 gctgggaagc ctgaacctaa tgcagtcact aaagcagccg gctccattgc gagtgcacag
     2221 aaaccgcctg ctgggaaagt ccagatagta tccaaaaaag tgagctacag tcatattcaa
     2281 tccaagtgtg tttccaagga caatattaag catgtccctg gatgtggcaa tgttcagatt
     2341 cagaacaaga aagtggacat atccaaggtc tcctccaagt gtgggtccaa agctaatatc
     2401 aagcacaagc ctggtggagg agatgtcaag attgaaagtc agaagttgaa cttcaaggag
     2461 aaggcccaag ccaaagtggg atagggatcc accggatcta gataactgat cataatcagc
     2521 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2581 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2641 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2701 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     2761 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2821 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2881 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2941 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3001 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3061 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3121 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3181 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3301 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3361 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3421 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3481 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3541 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3601 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3661 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     3721 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3781 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3841 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3901 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3961 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4021 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4081 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4141 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4201 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4261 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4321 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4381 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4441 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4501 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     4561 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4621 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4681 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4741 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     4801 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     4861 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     4921 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     4981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5821 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5881 gttccg
//
