LOCUS       sequence-226444-       16630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16630)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 16630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-163
FEATURES             Location/Qualifiers
     source          1..16630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(191..193,194..196,197..907)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(236..257)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(497..516)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     844..865
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      928..1022
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1066..1725
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1132..1151)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1790..1876
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1987..2152
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(2585..3535)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3583..3805
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     4488..4507
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            4545..4654
                     /note="incP origin of transfer"
     primer_bind     4677..4696
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4825..5616,5617..5685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5448..5467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5686..5777)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5796..5823)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5915..6001)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6154..6171
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12862..13146)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12920..12939)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             13173..14051
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(14489..15688)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
       61 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
      121 cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga tactagtgga
      181 ggaagaaaaa atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      241 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      301 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
      361 ctggcccacc ctcgtgacca ccctgggcta cggcgtgcag tgcttcgccc gctaccccga
      421 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      481 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      541 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      601 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
      661 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
      721 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      781 cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga
      841 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      901 gtacaagtaa gtaattagct gagcttggac tcctgttgat agatccagta atgacctcag
      961 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     1021 atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1081 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1141 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1201 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1261 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     1321 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1381 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1441 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     1501 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1561 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1621 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     1681 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1741 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg
     1801 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1861 cctgagtagg acaaatccgc cgctaggagc ttgcggccgc caggggatct gattaattaa
     1921 aacgtggctt actaggatcc gatatcattt aaatctaggg ataacagggt aatactagtg
     1981 tcgaccaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     2041 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     2101 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca caggctgaaa
     2161 gcgctatttc ttccagaatt gccatgattt tttccccacg ggaggcgtca ctggctcccg
     2221 tgttgtcggc agctttgatt cgataagcag catcgcctgt ttcaggctgt ctatgtgtga
     2281 ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt
     2341 actggtttca cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg
     2401 ttcatggtga acagctttaa atgcaccaaa aactcgtaaa agctctgatg tatctatctt
     2461 ttttacaccg ttttcatctg tgcatatgga cagttttccc tttgatatgt aacggtgaac
     2521 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
     2581 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
     2641 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
     2701 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
     2761 cgttatgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
     2821 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
     2881 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
     2941 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
     3001 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
     3061 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
     3121 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
     3181 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
     3241 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
     3301 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
     3361 tcatctgaac gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
     3421 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
     3481 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
     3541 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
     3601 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
     3661 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
     3721 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
     3781 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
     3841 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
     3901 gcaccctcgc aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac
     3961 ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat
     4021 gggggtaaat ggcactacag gcgcctttta tggattcatg caaggaaact acccataata
     4081 caagaaaagc ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct
     4141 atctgacttt ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg
     4201 attatcccgt gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc
     4261 aacaggctta cccgtcttac tgtcggggat cgacgctctc ccttatgcga ctcctgcatt
     4321 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
     4381 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
     4441 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
     4501 atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cgccgggcag gataggtgaa
     4561 gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg
     4621 ctcaacggga atcctgctct gcgaggctgg ccggcggcga acgtggcgag aaaggaaggg
     4681 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4741 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtaaatcaa tctaaagtat
     4801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4921 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5041 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5101 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     5161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     5461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5701 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5761 ttagaaaaat aaacaaaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     5821 tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     5881 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     5941 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     6001 gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg
     6061 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc
     6121 aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc
     6181 ttctctcatc cgccaaaaca gccaagcttg catgcctgca ggtcgactct agaggatccc
     6241 ctaaaaataa tgaccgagct agctctaact gtgggatgtg ataacgagtc ttgctatatg
     6301 gtgtttcagc ttcgtaaata aatgaactct gcgctccatc cttgttgcgt acggaagcaa
     6361 aggacgagag attaggaaaa tcgattattt tggctcgttg aagatccgat gcttcaaact
     6421 caatcacacg atccgcctta tttatttgct ttgttgtact gttaaccacc ttagctcgac
     6481 ttaataaagg caaatgagta aatcgagtcc atttccgttc ttgcattgga ttaaaccaaa
     6541 caaaaatacg ccactcctta tgacccgagt tacggaataa atgaccaata tgtacaacct
     6601 gcacattgtc attaaatgta gctagcctaa ccactcactc ttccccataa accatttcca
     6661 agaatctttg tgcttcctca gttatccgct ctttagataa cgttgcactt cttaataatc
     6721 gccagcgcct aagctcaaca tcttcctgtt taagcttaat aaaagcttgg ctaatccgtc
     6781 taatttgata atcttccaca ctctctgagt aacgctttaa gcaaagcgct accaaaggca
     6841 gtttctgtag attttttgca agagaggttc cgttaggagt ttgctttaac agccagctcg
     6901 atgtcgctct tgggtgatca aggctactat ctagacgctt tatgattctt aatagttgtc
     6961 gtacagcaat tcgatctctt tggttccaat caactctagg ggcgggtgcc agacgctctt
     7021 gttgatgctg ttgattccag tgaactagcc aatccctgtc atgtcggtaa agccaagcgt
     7081 atagcacccc accctctaaa gactgtcttg ccgccttaat tccttggtat ttatgcacta
     7141 gttgctgcca gtctttatgc ttaacagata aatcttcaga gttaggttgc acagactggc
     7201 taacaggtct tgtcgttata gagtgctcag taagagcact tgcctgctgt agcgcttcta
     7261 taaccgttag ttttggcaat aaggcttgcc acacaatact atgctgtaaa taactaaagg
     7321 cttttctatg cttacggaat atacttttca gccaacacgt gtccttgttc tctgccaact
     7381 ttaaatccag tttctctagt gcctcatcac taaaagtctg cctcactctc tccgccacca
     7441 agtcatgacg aatgtgcttg cttttggtta gccctagatc ctgcgctaag cgctgataaa
     7501 acagcgtcca ctgctcaagg cttggggaaa gctcttgcgc tcgtggagca tctaacagag
     7561 gggctatata agcagctagt gctgttaatt gagataggga gtctttgggg tagtctgaaa
     7621 gcagctcagt atgacccaaa gcccaaaatt gatgtcggtg atcatctaca gctctatcaa
     7681 agaagactaa agcaccgtgt tttggacaat atggcaaagc gggcaaatac caatctcgtt
     7741 gccaaaaggc ttccccatac ctatttagct gaagagcaac gcaatcaggg cagtatctaa
     7801 agcggttatc gctcttaact ctagaagcag cgactcctag cattaaatgc accgcacctt
     7861 gcgcttggta ctccattaac cgaatagctt cgtctcggcg ctccttgcct acaaacggag
     7921 catataaagg gaataaggta tgctcataaa taagctgctg aacagcgtaa cgtcctgttt
     7981 gatgtagatg tcttgctatc acacctaaat gcgagggcag acctaaggta gcgaccacct
     8041 tgcggttgcc atacacctca tccaacagct gcttaggact aacaatccct tgataaacgc
     8101 ctgcccgtgc aatagtgcta taaatcagct cattcgagta cggaacagga aagtttctca
     8161 tggctaacct gcttgtttaa ataagctcgc catatccact attacccctt tcccttttaa
     8221 ccgttcatgc atggtttttt caggttggcg ttgtgaatag atataacgta aatccgtatc
     8281 aggcaagcta tcccagtcgc tgggcttgac tacctttata gccgaaacct ttttactctt
     8341 ggagggcttt tctagttctg acactaccgt ttcgccttcc atcaaccact gcaaaacaag
     8401 aggcagtaac ttttgtcttg tcatcgttgg attctggcta aacgcttttt taatagtggg
     8461 aattaacagg cttgaatcgt aatcctcttt cagcatcaga tataaatgac gctgatcttc
     8521 ggtatctaac tcttgaaggg ctttttcttc tggtgtttgt tcttgtatcg ctgcgatatc
     8581 tagctgaagt tggattaacc gtttatcaat ctcgggaacg actagatcag aataacgagc
     8641 aatgcgttct gggatacccg agcgtaatgc ctctagcatg gggtgcacag gctttaactc
     8701 atcttgatac acttgccgca ataaaccagc ggtaatacgc tcattgccta aagctagcgc
     8761 acggagctga gcgagtacaa aaagttttac tacaatgtcc atcactcctt ggcttagctc
     8821 ataccacaca tcacggacct catccgataa cagcgcatct ttgcgttgta aaagctgtaa
     8881 ttgccaaaga ttatccgtaa aagcgatcca ctcttgattg ggctttccac gttgcgtttg
     8941 ttgtatagga tcccagaata tagctccaaa ccctgcccct ctacgtgcag accgcaaatc
     9001 agcctcaaaa atctctcgtg ctttaggggt accaatcaac atcactggta cgccaataat
     9061 attcaccatc gtcacaaaaa agttcagcat ctcttgagat ccacccgaac gagagcggct
     9121 taaatgctga atttcatcaa taaccaacaa ccctaaagca tgtgcattgg ctatttgcga
     9181 catcaaagcc aacatggttt ctataccatg acgttttaag ccataacgac gctcatagtt
     9241 cgagcccaag gctcgatcca acgctctgaa aaaattcaag cagatttctt ttagcgaacc
     9301 attatgcgag cagtctattt tcaaatacac cacctgctct acattgagtt cacgatggta
     9361 aatcacctga ggatacgtgg ctagaatacg atgaagagag gtcgtcttcc cactaccaga
     9421 acaaccaatt aacaataagc tttgtgccgt agatcgtgcc tcctcaaagc gaaatgtctc
     9481 caactctccc gtttgaacac gctcataacc attttgtaaa tgcttttgta aatctcctgt
     9541 tttaggattt ctgcctacgt agccacctcg aatcatgacc gaaatacgct cacttagtag
     9601 caaatgcgta cctaatggct gaaaatagtc atctggaata cgacaaatgg tatgagctct
     9661 gataacacgg gacttttgca agtcagagga agtaagctgt aaagaggatt tcagtgatgc
     9721 agcactattc actgactctt gtaatggtgg taaggcctcg ataaaaggat tatcacgata
     9781 agcctctacc cccgtatcac gataaactgc ttgaatccgg gtagcactca tgactcatcc
     9841 ttttctggtg gatcctgaaa taattcaggc acgtatgtgg gtaggctgta atcttcttga
     9901 tcatccgctt ccactgcgtt gaaaggaata actttagcct catcacctga agagcttggc
     9961 ttcaaatgct ccgcacgttt tttacgctcc gaggtcacgg cttctttttt attagtttta
    10021 atctgcttaa tgcgtgttga tttgggctca gtagtactgg gcgttaactt attcgctttc
    10081 tgaatggttt gctgaataaa cgcctcaagc tccctgcgtt tagttaactc atcctgcttc
    10141 gcattggctt tattgtgttt ttcttgtgct tgtatatccc aaacctccca aaatgagaga
    10201 cctttaaact gccgactacg ttccgtcaga ttacagcgcc aaaatacacg gctgccaact
    10261 tgcggaaaca aataaatcgt atcaaccagc actgggtcat aagccgcttc taaatgttga
    10321 ggtctagcta tatcagtgct ccgctgcaac caaccctcac gcagaatctc tgaccccgag
    10381 taatacaaac cccacaaatt aacgccaaat gaagaaatag agacctttcg gcgaggcagt
    10441 aacgctactc gcaactgctc ttgctccaca gcccttaaac tacctgtacg atgctgcata
    10501 ccccattgcc atagctggac aggaatagac ggtaaatctg taggaaaatc agcatctcga
    10561 tcgtatttat ccatcaccag atggttattt ctgaataaga tcgtacgcaa aataatttgt
    10621 gtgaactcaa ataccgacag agatgcatct aacctatagt ctgtttcacc atggcttttg
    10681 atccgactgc cctctacaat gccaggtgca aaggacttaa actcggcttg tagtgttcta
    10741 aaagtgcttt ccactatgcc tttagcatcg ccacgtctag gtggagcact ttccactcgc
    10801 acattaaaac tagaaactaa ggcttcgacc tgatgactca ttaattcgcc acggtccgct
    10861 agcaacacat ctggcaaacc tacacacggc cagtctgagc tactaatctc aatatcatgc
    10921 tgggcacaaa tggccgtttt gtcagagcaa gcatttacaa aagcctgcat cgccaccaca
    10981 taagacggat tttcaaagcc gatataaaag cccgtgatca tccgactaaa cacatcaatc
    11041 acaatgtaaa gcgttggtct tcctatgatt ttttggcgat catgatgatc cactaaataa
    11101 atatcagcaa tcgtggcatc aatctcataa cgactcccag ggcctaacgc ctgagaagtg
    11161 gctgtactac ttaagggtcg tacgtctttt ttatataccc ctgctttaac tctagacttt
    11221 aagcgctgag ctttagggta ttctcgatca taaaaataac gaaactgacg tagtgttggg
    11281 taatcctctt ggggaatgcg aggaaaatac tgagcaaaca agtccacaaa tcgtctatag
    11341 gcaacggtgg tctttgtacc tttttgattt aacaggtgct tttctatggt caacctaaaa
    11401 aggcgttcaa tctcgggcgt taccttggtt ccttcaccct taccatattc tctggctcgg
    11461 ccaatctttg ctgttcctgt cgctgaacgt ctttcccctg gtgcaccgct gtttttgtag
    11521 tcaggaatta atgcattagg cgtttgacca cgctgccagt aacggcgtaa caacttataa
    11581 actgtagcct tagtaacctt atgttcttgg accacatgct caacgagttc gcttctgact
    11641 ttagggtcga aacgatcctt actattaata ataggaagaa tttttcgata atcctcctcg
    11701 cgcttctgga agctgacaga atctacagac ggctcttcta aatcaagatg tacataaggg
    11761 tcatcagttc taactaagcg gccttcatct aaatactgca ttagcaacaa ctcagcccta
    11821 gcttgtggaa ctcctttatc agcgcttatt tgcatccaga caacttggcc atcctctata
    11881 gccaaaatgc gatacggatc attatcaaat agcacaacct cattaatttg ccacatagcg
    11941 caactcctcc atattcacta cttggctaat acagagatct gcacacttat ttgccctgaa
    12001 agacttataa atattgaact taataaaacc atttgcggtt aaggctcgta tctcactcaa
    12061 tgttttgcct aactccaaat cataagcaat atcaacctgc ttacagacat tgataatgtt
    12121 ttcatctcct ttttcttgca ggatatgggc caatggggat agttgtgcta aaagctccgc
    12181 agaaacttct tctgttttca ctgaataaag ccattcaata ttttctttta ctacgggatt
    12241 tatttcttta tcagtaaaaa tgaaccaagg aatttgcttt tgctgccaat agcgacgctc
    12301 tagttctagt ttttctaagg tacgctcgtc ttgtaaggct gctgcaggtt tgacttgaat
    12361 agcaaactgc tcaaaaggac catctttgca gtccactaaa aaatcagtag acataacctg
    12421 atctacacca cgaataacag gatgcttaat accactatct attgcaatct gcctggtatc
    12481 actaggtaat aaggggaact gctcgcgtat atctagcacg ctgctctccc actcaagact
    12541 gagaaaaaca gcaagctcta agtcagatag caaatgatgg actcgtcccg tcttatgaga
    12601 ataaatacgg tgggaacgac ctgaagaagg aacttcttgt actgttagcc atggaatata
    12661 gtctttacca tgcccttggc cacgcccctc tttaatacga cgggcaattt gcacttcaga
    12721 aaatgaagag tttgctttag ccataacaaa agtccagtat gctttttcac agcataactg
    12781 gactgatttc agtttacaac tattctgtct agtttaagac ttgggtaccg agctcgaatt
    12841 cgctagccca aaaaaacggg tatggagaaa cagtagagag ttgcgataaa aagcgtcagg
    12901 taggatccgc taatcttatg gataaaaatg ctatggcata gcaaagtgtg acgccgtgca
    12961 aataatcaat gtggactttt ctgccgtgat tatagacact tttgttacgc gtttttgtca
    13021 tggctttggt cccgctttgt tacagaatgc ttttaataag cggggttacc ggtttggtta
    13081 gcgagaagag ccagtaaaag acgcagtgac ggcaatgtct gatgcaatat ggacaattgg
    13141 tttcttctct gaatggcggg agtatgaaaa gtatggctga agcgcaaaat gatcccctgc
    13201 tgccgggata ctcgtttaat gcccatctgg tggcgggttt aacgccgatt gaggccaacg
    13261 gttatctcga tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca
    13321 ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga atttgtttgc cgaccgggtg
    13381 atattttgct gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg
    13441 aatggtatca ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact
    13501 ggccgtcaat atttgccaat acggggttct ttcgcccgga tgaagcgcac cagccgcatt
    13561 tcagcgacct gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc
    13621 tgctggcgat aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt
    13681 cgctccatcc accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc
    13741 tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcttg tcgccgtcgc
    13801 gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc
    13861 aacgtatcag ccaggcgaag ctgcttttga gcaccacccg gatgcctatc gccaccgtcg
    13921 gtcgcaatgt tggttttgac gatcaactct atttctcgcg ggtatttaaa aaatgcaccg
    13981 gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca
    14041 agttgtcata attggtaacg aatcagacaa ttgacggctt gacggagtag catagggttt
    14101 gcagaatccc tgcttcgtcc atttgacagg cacattatgc atcgatgata agctgtcaaa
    14161 catgagcaga tcctctacgc cggacgcatc gtggcctgtg ggcggacaat aaagtcttaa
    14221 actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag aatagttgta
    14281 aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag
    14341 caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggggtcg
    14401 acgcggccgt ggcgcgcctc ctaggtgctc gagtggcggc cgctattgac ccataaactg
    14461 ccaggaattg gggatcggat tcgagctctc agcgatcggc tcgttgccct gcgccgctcc
    14521 aaagcccgcg acgcagcgcc ggcaggcaga gcaagtagag ggcagcgcct gcaatccatg
    14581 cccacccgtt ccacgttgtt atagaagccg catagatcgc cgtgaagagg aggggtccga
    14641 cgatcgaggt caggctggtg agcgccgcca gtgagccttg cagctgcccc tgacgttcct
    14701 catccacctg cctggacaac attgcttgca gcgccggcat tccgatgcca cccgaagcaa
    14761 gcaggaccat gatcgggaac gccatccatc cccgtgtcgc gaaggcaagc aggatgtagc
    14821 ctgtgccgtc ggcaatcatt ccgagcatga gtgcccgcct ttcgccgagc cgggcggcta
    14881 cagggccggt gatcattgcc tgggcgagtg aatgcagaat gccaaatgcg gcaagcgaaa
    14941 tgccgatcgt ggtcgcgtcc cagtgaaagc gatcctcgcc gaaaatgacc caaagcgcgg
    15001 ccggcacctg tccgacaagt tgcatgatga agaagaccgc catcagggcg gcgacgacgg
    15061 tcatgccccg ggcccaccgg aacgaagcga gcgggttgag agcctcccgg cgtaacggcc
    15121 ggcgttcgcc tttgtgcgac tccggcaaaa ggaaacagcc cgtcaggaaa ttgaggccgt
    15181 tcaaggctgc cgcggcgaag aacggagcgt ggggggagaa accgcccatc agcccaccga
    15241 gcacaggtcc cgcgaccatc ccgaacccga aacaggcgct catgaagccg aagtgccgcg
    15301 cgcgctcatc gccatcagtg atatcggcaa tataagcgcc ggctaccgcc ccagtcgccc
    15361 cggtgatgcc ggccacgatc cgcccgatat agagaaccca aaggaaaggc gctgtcgcca
    15421 tgatggcgta gtcgacagtg gcgccggcca gcgagacgag caagattggc cgccgcccga
    15481 aacgatccga cagcgcgccc agcacaggtg cgcaggcaaa ttgcaccaac gcatacagcg
    15541 ccagcagaat gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc
    15601 ccggcagcac cggcataatc aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa
    15661 ttacgatcag gggtatgttg ggtttcacgt ctggcctccg gaccagcctc cgctggtccg
    15721 attgaacgcg cggattcttt atcactgata agttggtgga catattatgt ttatcagtga
    15781 taaagtgtca agcatgacaa agttgcagcc gaatacagtg atccgtgccg ccctggacct
    15841 gttgaacgag gtcggcgtag acggtctgac gacacgcaaa ctggcggaac ggttgggggt
    15901 tcagcagccg gcgctttact ggcacttcag gaacaagcgg gcgctgctcg acgcactggc
    15961 cgaagccatg ctggcggaga atcatacgca ttcggtgccg agagccgacg acgactggcg
    16021 ctcatttctg atcgggaatg cccgcagctt caggcaggcg ctgctcgcct accgcgatgg
    16081 cgcgcgcatc catgccggca cgcgaccggg cgcaccgcag atggaaacgg ccgacgcgca
    16141 gcttcgcttc ctctgcgagg cgggtttttc ggccggggac gccgtcaatg cgctgatgac
    16201 aatcagctac ttcactgttg gggccgtgct tgaggagcag gccggcgaca gcgatgccgg
    16261 cgagcgcggc ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg ccgcgataga
    16321 cgccttcgac gaagccggtc cggacgcagc gttcgagcag ggactcgcgg tgattgtcga
    16381 tggattggcg aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa agggtgctaa
    16441 gaaaccatta ttatcatgaa gctcgcacgc tgccgcaagc actcagggcg caagggctgc
    16501 taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
    16561 gtcagctact gggctatctg gacaatggaa aacgcaagcg caaagagaaa gcaggtagct
    16621 tgcagtgggc
//
