LOCUS       sequence-226449-       16624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16624)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 16624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-161
FEATURES             Location/Qualifiers
     source          1..16624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..108
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             152..811
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(218..237)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      876..962
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1391..2269)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2296..2580
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2503..2522
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(9271..9288)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      9441..9527
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9619..9646
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        9665..9756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9757..9825,9826..10617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9975..9994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(10746..10765)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            10788..10897
                     /note="incP origin of transfer"
     primer_bind     complement(10935..10954)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      11637..11859
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             11907..12857
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  13290..13455
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(13575..14774)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             join(15908..15910,15911..16624)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(15950..15971)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(16211..16230)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16558..16579
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
       61 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagctagctt
      121 ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      181 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      241 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      301 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      361 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      421 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
      481 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
      541 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
      601 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
      661 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
      721 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
      781 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
      841 aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag gctcagtcga
      901 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      961 atccgccgct aggagcttgc ggccgccagg ggtcaatagc ggccgccact cgagcaccta
     1021 ggaggcgcgc cacggccgcg tcgaccccac gcccctcttt aatacgacgg gcaatttgca
     1081 cttcagaaaa tgaagagttt gctttagcca taacaaaagt ccagtatgct ttttcacagc
     1141 ataactggac tgatttcagt ttacaactat tctgtctagt ttaagacttt attgtcatag
     1201 tttagatcta ttttgttcag tttaagactt tattgtccgc ccacaggcca cgatgcgtcc
     1261 ggcgtagagg atctgctcat gtttgacagc ttatcatcga tgcataatgt gcctgtcaaa
     1321 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
     1381 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
     1441 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
     1501 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
     1561 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
     1621 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
     1681 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
     1741 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
     1801 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
     1861 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
     1921 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
     1981 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
     2041 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
     2101 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
     2161 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     2221 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     2281 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     2341 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     2401 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     2461 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     2521 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     2581 acccgttttt ttgggctagc gaattcgagc tcggtaccca agtcttaaac tagacagaat
     2641 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
     2701 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
     2761 ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt tccttcttca
     2821 ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt gctatctgac
     2881 ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga tatacgcgag
     2941 cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg tattaagcat
     3001 cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga ctgcaaagat
     3061 ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca agacgagcgt
     3121 accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat tccttggttc
     3181 atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg gctttattca
     3241 gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt ggcccatatc
     3301 ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga tattgcttat
     3361 gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa tggttttatt
     3421 aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg tattagccaa
     3481 gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc tatttgataa
     3541 tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc aaataagcgc
     3601 tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt tagatgaagg
     3661 ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc cgtctgtaga
     3721 ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta ttattaatag
     3781 taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg tccaagaaca
     3841 taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc gtggtcaaac
     3901 gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa gacgttcagc
     3961 gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag gaaccaaggt
     4021 aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt taaatcaaaa
     4081 aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc agtattttcc
     4141 tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt atgatcgaga
     4201 ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata aaaaagacgt
     4261 acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc gttatgagat
     4321 tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa aaatcatagg
     4381 aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg gcttttatat
     4441 cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg cttgctctga
     4501 caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact ggccgtgtgt
     4561 aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc aggtcgaagc
     4621 cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg gcgatgctaa
     4681 aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct ttgcacctgg
     4741 cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt tagatgcatc
     4801 tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca gaaataacca
     4861 tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac cgtctattcc
     4921 tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg ctgtggagca
     4981 agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt catttggcgt
     5041 taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt ggttgcagcg
     5101 gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag tgctggttga
     5161 tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta atctgacgga
     5221 acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac aagcacaaga
     5281 aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg agcttgaggc
     5341 gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta ctgagcccaa
     5401 atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct cggagcgtaa
     5461 aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag ttattccttt
     5521 caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg tgcctgaatt
     5581 atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca agcagtttat
     5641 cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt accaccatta
     5701 caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac ttcctctgac
     5761 ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga tgactatttt
     5821 cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat tcgaggtggc
     5881 tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa tggttatgag
     5941 cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc tacggcacaa
     6001 agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca tcgtattcta
     6061 gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt ggtgtatttg
     6121 aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt tttcagagcg
     6181 ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg tcatggtata
     6241 gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg gttgttggtt
     6301 attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga gatgctgaac
     6361 ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg tacccctaaa
     6421 gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg gtttggagct
     6481 atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga gtggatcgct
     6541 tttacggata atctttggca attacagctt ttacaacgca aagatgcgct gttatcggat
     6601 gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt agtaaaactt
     6661 tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac cgctggttta
     6721 ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga ggcattacgc
     6781 tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga gattgataaa
     6841 cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc agaagaaaaa
     6901 gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct gaaagaggat
     6961 tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa tccaacgatg
     7021 acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga aacggtagtg
     7081 tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt agtcaagccc
     7141 agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca acgccaacct
     7201 gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat ggcgagctta
     7261 tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat gagctgattt
     7321 atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag cagctgttgg
     7381 atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg catttaggtg
     7441 tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt atttatgagc
     7501 ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac gaagctattc
     7561 ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc gctgcttcta
     7621 gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt cagctaaata
     7681 ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca tattgtccaa
     7741 aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat caattttggg
     7801 ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct caattaacag
     7861 cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag ctttccccaa
     7921 gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg ctaaccaaaa
     7981 gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt agtgatgagg
     8041 cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg ctgaaaagta
     8101 tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg tggcaagcct
     8161 tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt actgagcact
     8221 ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat ttatctgtta
     8281 agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg gcaagacagt
     8341 ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat tggctagttc
     8401 actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt gattggaacc
     8461 aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta gatagtagcc
     8521 ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac ggaacctctc
     8581 ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt tactcagaga
     8641 gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt aaacaggaag
     8701 atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa gagcggataa
     8761 ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg gttaggctag
     8821 ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt aactcgggtc
     8881 ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa tggactcgat
     8941 ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca aagcaaataa
     9001 ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc aaaataatcg
     9061 attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg cagagttcat
     9121 ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca cagttagagc
     9181 tagctcggtc attattttta ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     9241 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     9301 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     9361 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     9421 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     9481 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     9541 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     9601 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     9661 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9721 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9781 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9841 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9901 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9961 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    10021 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10081 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10141 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10201 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10261 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
    10321 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10381 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10441 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10501 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10561 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10621 tcagaccaag tttactcata tatactttag attgatttac gcgccctgta gcggcgcatt
    10681 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    10741 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccgccg gccagcctcg
    10801 cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca
    10861 cccgctcgcg ggtgggccta cttcacctat cctgcccggc ggcatcaccg gcgccacagg
    10921 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
    10981 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
    11041 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
    11101 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gatccccgac
    11161 agtaagacgg gtaagcctgt tgatgatacc gctgccttac tgggtgcatt agccagtctg
    11221 aatgacctgt cacgggataa tccgaagtgg tcagactgga aaatcagagg gcaggaactg
    11281 ctgaacagca aaaagtcaga tagcaccaca tagcagaccc gccataaaac gccctgagaa
    11341 gcccgtgacg ggcttttctt gtattatggg tagtttcctt gcatgaatcc ataaaaggcg
    11401 cctgtagtgc catttacccc cattcactgc cagagccgtg agcgcagcga actgaatgtc
    11461 acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt
    11521 tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg ttttgtagag
    11581 gagcaaacag cgtttgcgac atccttttgt aatactgcgg aactgactaa agtagtgagt
    11641 tatacacagg gctgggatct attcttttta tcttttttta ttctttcttt attctataaa
    11701 ttataaccac ttgaatataa acaaaaaaaa cacacaaagg tctagcggaa tttacagagg
    11761 gtctagcaga atttacaagt tttccagcaa aggtctagca gaatttacag atacccacaa
    11821 ctcaaaggaa aaggactagt aattatcatt gactagccca tctcaattgg tatagtgatt
    11881 aaaatcacct agaccaattg agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac
    11941 tagcgattag tcgctatgac ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg
    12001 cactactcaa ccccacgatt gaaaacccta caaggaaaga acggacggta tcgttcactt
    12061 ataaccaata cgttcagatg atgaacatca gtagggaaaa tgcttatggt gtattagcta
    12121 aagcaaccag agagctgatg acgagaactg tggaaatcag gaatcctttg gttaaaggct
    12181 ttgagatttt ccagtggaca aactatgcca agttctcaag cgaaaaatta gaattagttt
    12241 ttagtgaaga gatattgcct tatcttttcc agttaaaaaa attcataaaa tataatctgg
    12301 aacatgttaa gtcttttgaa aacaaatact ctatgaggat ttatgagtgg ttattaaaag
    12361 aactaacaca aaagaaaact cacaaggcaa atatagagat tagccttgat gaatttaagt
    12421 tcatgttaat gcttgaaaat aactaccatg agtttaaaag gcttaaccaa tgggttttga
    12481 aaccaataag taaagattta aacacttaca gcaatatgaa attggtggtt gataagcgag
    12541 gccgcccgac tgatacgttg attttccaag ttgaactaga tagacaaatg gatctcgtaa
    12601 ccgaacttga gaacaaccag ataaaaatga atggtgacaa aataccaaca accattacat
    12661 cagattccta cctacataac ggactaagaa aaacactaca cgatgcttta actgcaaaaa
    12721 ttcagctcac cagttttgag gcaaaatttt tgagtgacat gcaaagtaag tatgatctca
    12781 atggttcgtt ctcatggctc acgcaaaaac aacgaaccac actagagaac atactggcta
    12841 aatacggaag gatctgaggt tcttatggct cttgtatcta tcagtgaagc atcaagacta
    12901 acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa agggaaaact gtccatatgc
    12961 acagatgaaa acggtgtaaa aaagatagat acatcagagc ttttacgagt ttttggtgca
    13021 tttaaagctg ttcaccatga acagatcgac aatgtaacag atgaacagca tgtaacacct
    13081 aatagaacag gtgaaaccag taaaacaaag caactagaac atgaaattga acacctgaga
    13141 caacttgtta cagctcaaca gtcacacata gacagcctga aacaggcgat gctgcttatc
    13201 gaatcaaagc tgccgacaac acgggagcca gtgacgcctc ccgtggggaa aaaatcatgg
    13261 caattctgga agaaatagcg ctttcagcct gtgggcggac aaaatagttg ggaactggga
    13321 ggggtggaaa tggagttttt aaggattatt tagggaagag tgacaaaata gatgggaact
    13381 gggtgtagcg tcgtaagcta atacgaaaat taaaaatgac aaaatagttt ggaactagat
    13441 ttcacttatc tggttggtcg acactagtat taccctgtta tccctagatt taaatgatat
    13501 cggatcctag taagccacgt tttaattaat cagatcccat aaactgccag gaattgggga
    13561 tcggattcga gctctcagcg atcggctcgt tgccctgcgc cgctccaaag cccgcgacgc
    13621 agcgccggca ggcagagcaa gtagagggca gcgcctgcaa tccatgccca cccgttccac
    13681 gttgttatag aagccgcata gatcgccgtg aagaggaggg gtccgacgat cgaggtcagg
    13741 ctggtgagcg ccgccagtga gccttgcagc tgcccctgac gttcctcatc cacctgcctg
    13801 gacaacattg cttgcagcgc cggcattccg atgccacccg aagcaagcag gaccatgatc
    13861 gggaacgcca tccatccccg tgtcgcgaag gcaagcagga tgtagcctgt gccgtcggca
    13921 atcattccga gcatgagtgc ccgcctttcg ccgagccggg cggctacagg gccggtgatc
    13981 attgcctggg cgagtgaatg cagaatgcca aatgcggcaa gcgaaatgcc gatcgtggtc
    14041 gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa gcgcggccgg cacctgtccg
    14101 acaagttgca tgatgaagaa gaccgccatc agggcggcga cgacggtcat gccccgggcc
    14161 caccggaacg aagcgagcgg gttgagagcc tcccggcgta acggccggcg ttcgcctttg
    14221 tgcgactccg gcaaaaggaa acagcccgtc aggaaattga ggccgttcaa ggctgccgcg
    14281 gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc caccgagcac aggtcccgcg
    14341 accatcccga acccgaaaca ggcgctcatg aagccgaagt gccgcgcgcg ctcatcgcca
    14401 tcagtgatat cggcaatata agcgccggct accgccccag tcgccccggt gatgccggcc
    14461 acgatccgcc cgatatagag aacccaaagg aaaggcgctg tcgccatgat ggcgtagtcg
    14521 acagtggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg atccgacagc
    14581 gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag cagaatgcca
    14641 tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg cagcaccggc
    14701 ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac gatcaggggt
    14761 atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg aacgcgcgga
    14821 ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa gtgtcaagca
    14881 tgacaaagtt gcagccgaat acagtgatcc gtgccgccct ggacctgttg aacgaggtcg
    14941 gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag cagccggcgc
    15001 tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa gccatgctgg
    15061 cggagaatca tacgcattcg gtgccgagag ccgacgacga ctggcgctca tttctgatcg
    15121 ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg cgcatccatg
    15181 ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt cgcttcctct
    15241 gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc agctacttca
    15301 ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag cgcggcggca
    15361 ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc ttcgacgaag
    15421 ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga ttggcgaaaa
    15481 ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgctaagaaa ccattattat
    15541 catgaagctc gcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa
    15601 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
    15661 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
    15721 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
    15781 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
    15841 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatact agtggaggaa
    15901 gaaaaaaatg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
    15961 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
    16021 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
    16081 caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc ccgaccacat
    16141 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
    16201 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
    16261 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
    16321 gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg acaagcagaa
    16381 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
    16441 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
    16501 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
    16561 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
    16621 gtaa
//
