LOCUS       sequence-226457-       15487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-155
FEATURES             Location/Qualifiers
     source          1..15487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..696
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      717..811
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             855..1514
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(921..940)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1579..1665
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1776..1941
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(2374..3324)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3372..3594
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     4277..4296
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            4334..4443
                     /note="incP origin of transfer"
     primer_bind     4466..4485
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4614..5405,5406..5474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5237..5256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5475..5566)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5585..5612)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5704..5790)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5943..5960
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12651..12935)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12709..12728)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12962..13840
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             14332..15126
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     GQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDVGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
      481 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccactcca ccggcggcat ggacgagctg tacaagtaag taattagctg agcttggact
      721 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      781 ttgccgccgg gcgtttttta ttggtgagaa tccaagctag cttggcgaga ttttcaggag
      841 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      901 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      961 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     1021 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
     1081 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     1141 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     1201 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     1261 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     1321 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     1381 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1441 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     1501 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga
     1561 ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1621 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctaggagct
     1681 tgcggccgcc aggggatctg attaattaaa acgtggctta ctaggatccg atatcattta
     1741 aatctaggga taacagggta atactagtgt cgaccaacca gataagtgaa atctagttcc
     1801 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc
     1861 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc
     1921 caactatttt gtccgcccac aggctgaaag cgctatttct tccagaattg ccatgatttt
     1981 ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc
     2041 atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt
     2101 tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca
     2161 tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttaaa tgcaccaaaa
     2221 actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac
     2281 agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat
     2341 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
     2401 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata
     2461 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
     2521 taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt
     2581 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
     2641 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
     2701 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
     2761 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
     2821 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
     2881 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
     2941 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
     3001 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
     3061 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
     3121 accataagca ttttccctac tgatgttcat catctgaacg tattggttat aagtgaacga
     3181 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
     3241 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
     3301 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
     3361 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat
     3421 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa
     3481 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
     3541 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
     3601 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa
     3661 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa
     3721 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
     3781 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
     3841 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
     3901 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
     3961 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
     4021 aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtcggggatc
     4081 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     4141 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     4201 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4261 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4321 cggtgatgcc gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     4381 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     4441 cggcggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     4501 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     4561 tacagggcgc gtaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4921 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5221 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaaagag tttgtagaaa
     5581 cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg
     5641 cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg
     5701 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     5761 gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg
     5821 gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag
     5881 gtgggaccac cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt
     5941 ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ccaagcttgc
     6001 atgcctgcag gtcgactcta gaggatcccc taaaaataat gaccgagcta gctctaactg
     6061 tgggatgtga taacgagtct tgctatatgg tgtttcagct tcgtaaataa atgaactctg
     6121 cgctccatcc ttgttgcgta cggaagcaaa ggacgagaga ttaggaaaat cgattatttt
     6181 ggctcgttga agatccgatg cttcaaactc aatcacacga tccgccttat ttatttgctt
     6241 tgttgtactg ttaaccacct tagctcgact taataaaggc aaatgagtaa atcgagtcca
     6301 tttccgttct tgcattggat taaaccaaac aaaaatacgc cactccttat gacccgagtt
     6361 acggaataaa tgaccaatat gtacaacctg cacattgtca ttaaatgtag ctagcctaac
     6421 cactcactct tccccataaa ccatttccaa gaatctttgt gcttcctcag ttatccgctc
     6481 tttagataac gttgcacttc ttaataatcg ccagcgccta agctcaacat cttcctgttt
     6541 aagcttaata aaagcttggc taatccgtct aatttgataa tcttccacac tctctgagta
     6601 acgctttaag caaagcgcta ccaaaggcag tttctgtaga ttttttgcaa gagaggttcc
     6661 gttaggagtt tgctttaaca gccagctcga tgtcgctctt gggtgatcaa ggctactatc
     6721 tagacgcttt atgattctta atagttgtcg tacagcaatt cgatctcttt ggttccaatc
     6781 aactctaggg gcgggtgcca gacgctcttg ttgatgctgt tgattccagt gaactagcca
     6841 atccctgtca tgtcggtaaa gccaagcgta tagcacccca ccctctaaag actgtcttgc
     6901 cgccttaatt ccttggtatt tatgcactag ttgctgccag tctttatgct taacagataa
     6961 atcttcagag ttaggttgca cagactggct aacaggtctt gtcgttatag agtgctcagt
     7021 aagagcactt gcctgctgta gcgcttctat aaccgttagt tttggcaata aggcttgcca
     7081 cacaatacta tgctgtaaat aactaaaggc ttttctatgc ttacggaata tacttttcag
     7141 ccaacacgtg tccttgttct ctgccaactt taaatccagt ttctctagtg cctcatcact
     7201 aaaagtctgc ctcactctct ccgccaccaa gtcatgacga atgtgcttgc ttttggttag
     7261 ccctagatcc tgcgctaagc gctgataaaa cagcgtccac tgctcaaggc ttggggaaag
     7321 ctcttgcgct cgtggagcat ctaacagagg ggctatataa gcagctagtg ctgttaattg
     7381 agatagggag tctttggggt agtctgaaag cagctcagta tgacccaaag cccaaaattg
     7441 atgtcggtga tcatctacag ctctatcaaa gaagactaaa gcaccgtgtt ttggacaata
     7501 tggcaaagcg ggcaaatacc aatctcgttg ccaaaaggct tccccatacc tatttagctg
     7561 aagagcaacg caatcagggc agtatctaaa gcggttatcg ctcttaactc tagaagcagc
     7621 gactcctagc attaaatgca ccgcaccttg cgcttggtac tccattaacc gaatagcttc
     7681 gtctcggcgc tccttgccta caaacggagc atataaaggg aataaggtat gctcataaat
     7741 aagctgctga acagcgtaac gtcctgtttg atgtagatgt cttgctatca cacctaaatg
     7801 cgagggcaga cctaaggtag cgaccacctt gcggttgcca tacacctcat ccaacagctg
     7861 cttaggacta acaatccctt gataaacgcc tgcccgtgca atagtgctat aaatcagctc
     7921 attcgagtac ggaacaggaa agtttctcat ggctaacctg cttgtttaaa taagctcgcc
     7981 atatccacta ttaccccttt cccttttaac cgttcatgca tggttttttc aggttggcgt
     8041 tgtgaataga tataacgtaa atccgtatca ggcaagctat cccagtcgct gggcttgact
     8101 acctttatag ccgaaacctt tttactcttg gagggctttt ctagttctga cactaccgtt
     8161 tcgccttcca tcaaccactg caaaacaaga ggcagtaact tttgtcttgt catcgttgga
     8221 ttctggctaa acgctttttt aatagtggga attaacaggc ttgaatcgta atcctctttc
     8281 agcatcagat ataaatgacg ctgatcttcg gtatctaact cttgaagggc tttttcttct
     8341 ggtgtttgtt cttgtatcgc tgcgatatct agctgaagtt ggattaaccg tttatcaatc
     8401 tcgggaacga ctagatcaga ataacgagca atgcgttctg ggatacccga gcgtaatgcc
     8461 tctagcatgg ggtgcacagg ctttaactca tcttgataca cttgccgcaa taaaccagcg
     8521 gtaatacgct cattgcctaa agctagcgca cggagctgag cgagtacaaa aagttttact
     8581 acaatgtcca tcactccttg gcttagctca taccacacat cacggacctc atccgataac
     8641 agcgcatctt tgcgttgtaa aagctgtaat tgccaaagat tatccgtaaa agcgatccac
     8701 tcttgattgg gctttccacg ttgcgtttgt tgtataggat cccagaatat agctccaaac
     8761 cctgcccctc tacgtgcaga ccgcaaatca gcctcaaaaa tctctcgtgc tttaggggta
     8821 ccaatcaaca tcactggtac gccaataata ttcaccatcg tcacaaaaaa gttcagcatc
     8881 tcttgagatc cacccgaacg agagcggctt aaatgctgaa tttcatcaat aaccaacaac
     8941 cctaaagcat gtgcattggc tatttgcgac atcaaagcca acatggtttc tataccatga
     9001 cgttttaagc cataacgacg ctcatagttc gagcccaagg ctcgatccaa cgctctgaaa
     9061 aaattcaagc agatttcttt tagcgaacca ttatgcgagc agtctatttt caaatacacc
     9121 acctgctcta cattgagttc acgatggtaa atcacctgag gatacgtggc tagaatacga
     9181 tgaagagagg tcgtcttccc actaccagaa caaccaatta acaataagct ttgtgccgta
     9241 gatcgtgcct cctcaaagcg aaatgtctcc aactctcccg tttgaacacg ctcataacca
     9301 ttttgtaaat gcttttgtaa atctcctgtt ttaggatttc tgcctacgta gccacctcga
     9361 atcatgaccg aaatacgctc acttagtagc aaatgcgtac ctaatggctg aaaatagtca
     9421 tctggaatac gacaaatggt atgagctctg ataacacggg acttttgcaa gtcagaggaa
     9481 gtaagctgta aagaggattt cagtgatgca gcactattca ctgactcttg taatggtggt
     9541 aaggcctcga taaaaggatt atcacgataa gcctctaccc ccgtatcacg ataaactgct
     9601 tgaatccggg tagcactcat gactcatcct tttctggtgg atcctgaaat aattcaggca
     9661 cgtatgtggg taggctgtaa tcttcttgat catccgcttc cactgcgttg aaaggaataa
     9721 ctttagcctc atcacctgaa gagcttggct tcaaatgctc cgcacgtttt ttacgctccg
     9781 aggtcacggc ttctttttta ttagttttaa tctgcttaat gcgtgttgat ttgggctcag
     9841 tagtactggg cgttaactta ttcgctttct gaatggtttg ctgaataaac gcctcaagct
     9901 ccctgcgttt agttaactca tcctgcttcg cattggcttt attgtgtttt tcttgtgctt
     9961 gtatatccca aacctcccaa aatgagagac ctttaaactg ccgactacgt tccgtcagat
    10021 tacagcgcca aaatacacgg ctgccaactt gcggaaacaa ataaatcgta tcaaccagca
    10081 ctgggtcata agccgcttct aaatgttgag gtctagctat atcagtgctc cgctgcaacc
    10141 aaccctcacg cagaatctct gaccccgagt aatacaaacc ccacaaatta acgccaaatg
    10201 aagaaataga gacctttcgg cgaggcagta acgctactcg caactgctct tgctccacag
    10261 cccttaaact acctgtacga tgctgcatac cccattgcca tagctggaca ggaatagacg
    10321 gtaaatctgt aggaaaatca gcatctcgat cgtatttatc catcaccaga tggttatttc
    10381 tgaataagat cgtacgcaaa ataatttgtg tgaactcaaa taccgacaga gatgcatcta
    10441 acctatagtc tgtttcacca tggcttttga tccgactgcc ctctacaatg ccaggtgcaa
    10501 aggacttaaa ctcggcttgt agtgttctaa aagtgctttc cactatgcct ttagcatcgc
    10561 cacgtctagg tggagcactt tccactcgca cattaaaact agaaactaag gcttcgacct
    10621 gatgactcat taattcgcca cggtccgcta gcaacacatc tggcaaacct acacacggcc
    10681 agtctgagct actaatctca atatcatgct gggcacaaat ggccgttttg tcagagcaag
    10741 catttacaaa agcctgcatc gccaccacat aagacggatt ttcaaagccg atataaaagc
    10801 ccgtgatcat ccgactaaac acatcaatca caatgtaaag cgttggtctt cctatgattt
    10861 tttggcgatc atgatgatcc actaaataaa tatcagcaat cgtggcatca atctcataac
    10921 gactcccagg gcctaacgcc tgagaagtgg ctgtactact taagggtcgt acgtcttttt
    10981 tatatacccc tgctttaact ctagacttta agcgctgagc tttagggtat tctcgatcat
    11041 aaaaataacg aaactgacgt agtgttgggt aatcctcttg gggaatgcga ggaaaatact
    11101 gagcaaacaa gtccacaaat cgtctatagg caacggtggt ctttgtacct ttttgattta
    11161 acaggtgctt ttctatggtc aacctaaaaa ggcgttcaat ctcgggcgtt accttggttc
    11221 cttcaccctt accatattct ctggctcggc caatctttgc tgttcctgtc gctgaacgtc
    11281 tttcccctgg tgcaccgctg tttttgtagt caggaattaa tgcattaggc gtttgaccac
    11341 gctgccagta acggcgtaac aacttataaa ctgtagcctt agtaacctta tgttcttgga
    11401 ccacatgctc aacgagttcg cttctgactt tagggtcgaa acgatcctta ctattaataa
    11461 taggaagaat ttttcgataa tcctcctcgc gcttctggaa gctgacagaa tctacagacg
    11521 gctcttctaa atcaagatgt acataagggt catcagttct aactaagcgg ccttcatcta
    11581 aatactgcat tagcaacaac tcagccctag cttgtggaac tcctttatca gcgcttattt
    11641 gcatccagac aacttggcca tcctctatag ccaaaatgcg atacggatca ttatcaaata
    11701 gcacaacctc attaatttgc cacatagcgc aactcctcca tattcactac ttggctaata
    11761 cagagatctg cacacttatt tgccctgaaa gacttataaa tattgaactt aataaaacca
    11821 tttgcggtta aggctcgtat ctcactcaat gttttgccta actccaaatc ataagcaata
    11881 tcaacctgct tacagacatt gataatgttt tcatctcctt tttcttgcag gatatgggcc
    11941 aatggggata gttgtgctaa aagctccgca gaaacttctt ctgttttcac tgaataaagc
    12001 cattcaatat tttcttttac tacgggattt atttctttat cagtaaaaat gaaccaagga
    12061 atttgctttt gctgccaata gcgacgctct agttctagtt tttctaaggt acgctcgtct
    12121 tgtaaggctg ctgcaggttt gacttgaata gcaaactgct caaaaggacc atctttgcag
    12181 tccactaaaa aatcagtaga cataacctga tctacaccac gaataacagg atgcttaata
    12241 ccactatcta ttgcaatctg cctggtatca ctaggtaata aggggaactg ctcgcgtata
    12301 tctagcacgc tgctctccca ctcaagactg agaaaaacag caagctctaa gtcagatagc
    12361 aaatgatgga ctcgtcccgt cttatgagaa taaatacggt gggaacgacc tgaagaagga
    12421 acttcttgta ctgttagcca tggaatatag tctttaccat gcccttggcc acgcccctct
    12481 ttaatacgac gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa
    12541 gtccagtatg ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta
    12601 gtttaagact tgggtaccga gctcgaattc gctagcccaa aaaaacgggt atggagaaac
    12661 agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg ataaaaatgc
    12721 tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc tgccgtgatt
    12781 atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt acagaatgct
    12841 tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga cgcagtgacg
    12901 gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga gtatgaaaag
    12961 tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg cccatctggt
    13021 ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg accgaccgct
    13081 gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg tgaaaaatca
    13141 gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag gagagattca
    13201 tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt actttcgtcc
    13261 gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata cggggttctt
    13321 tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa tcattaacgc
    13381 cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg agcaattgtt
    13441 actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata atcgggtacg
    13501 cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata tcgccagcgt
    13561 cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat cttttccgcc agcagttagg
    13621 gattagcgtc ttaagctggc gcgaggacca acgtatcagc caggcgaagc tgcttttgag
    13681 caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg atcaactcta
    13741 tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc gtgccggttg
    13801 tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa ttggtaacga atcagacaat
    13861 tgacggcttg acggagtagc atagggtttg cagaatccct gcttcgtcca tttgacaggc
    13921 acattatgca tcgatgataa gctgtcaaac atgagcagat cctctacgcc ggacgcatcg
    13981 tggcctgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat
    14041 aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa
    14101 agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc
    14161 ccgtcgtatt aaagaggggc gtggggtcga cgcggccgtg gcgcgcctcc taggtgctcg
    14221 agtggcggcc gctattgacc cataaactgc caggaattgg ggatcggatt cgagctaaga
    14281 attcccttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
    14341 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
    14401 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
    14461 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
    14521 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
    14581 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
    14641 gatggacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
    14701 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
    14761 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
    14821 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
    14881 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
    14941 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
    15001 tgcgtccggt cgatcaggga ggatgtcggg gaagaacagt atgtcgagct attttttgac
    15061 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
    15121 ttttagagct cgcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga
    15181 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
    15241 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
    15301 catggcgata gctagactgg gcggtcttat ggacagcaag cgaaccggaa ttgccagctg
    15361 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
    15421 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatac tagtggagga
    15481 agaaaaa
//
