LOCUS       sequence-226464-        5901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubule Associated Protein, Excitation: 400 / 504,
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5901)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-MAP4-N-10
FEATURES             Location/Qualifiers
     source          1..5901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1791..1800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1797..2507
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1942..1960)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    2629..2750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2666..2685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2720..2739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2757..3212)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2894..2915)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3106..3125
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3239..3343
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3345..3702
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3345..3536
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3368..3388)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3553..3688
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3737..4531
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3737..4531
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3791..3810)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4401..4420
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4719..4738)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4763..4810
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5139..5727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5628..5647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatggtgtc ccggcaagaa gaagcaaagg ctgctgtagg tgtgactgga aatgacatca
      601 ctaccccgcc aaacaaggag ccaccaccaa gcccagaaaa gaaagcaaag cctttggcca
      661 ccactcaacc tgcaaagact tcaacatcga aagccaaaac acagcccact tctctcccta
      721 agcaaccagc tcccaccacc tctggtgggt tgaataaaaa acccatgagc ctcgcctcag
      781 gctcagtgcc agctgcccca cacaaacgcc ctgctgctgc cactgctact gccaggcctt
      841 ccaccctacc tgccagagac gtgaagccaa agccaattac agaagctaag gttgccgaaa
      901 agcggacctc tccatccaag ccttcatctg ccccagccct caaacctgga cctaaaacca
      961 ccccaaccgt ttcaaaagcc acatctccct caactcttgt ttccactgga ccaagtagta
     1021 gaagtccagc tacaactctg cctaagaggc caaccagcat caagactgag gggaaacctg
     1081 ctgatgtcaa aaggatgact gctaagtctg cctcagctga cttgagtcgc tcaaagacca
     1141 cctctgccag ttctgtgaag agaaacacca ctcccactgg ggcagcaccc ccagcaggga
     1201 tgacttccac tcgagtcaag cccatgtctg cacctagccg ctcttctggg gctctttctg
     1261 tggacaagaa gcccacttcc actaagccta gctcctctgc tcccagggtg agccgcctgg
     1321 ccacaactgt ttctgcccct gacctgaaga gtgttcgctc caaggtcggc tctacagaaa
     1381 acatcaaaca ccagcctgga ggaggccggg ccaaggtaga gaaaaaaaca gaggcagcta
     1441 ccacagctgg gaagcctgaa cctaatgcag tcactaaagc agccggctcc attgcgagtg
     1501 cacagaaacc gcctgctggg aaagtccaga tagtatccaa aaaagtgagc tacagtcata
     1561 ttcaatccaa gtgtgtttcc aaggacaata ttaagcatgt ccctggatgt ggcaatgttc
     1621 agattcagaa caagaaagtg gacatatcca aggtctcctc caagtgtggg tccaaagcta
     1681 atatcaagca caagcctggt ggaggagatg tcaagattga aagtcagaag ttgaacttca
     1741 aggagaaggc ccaagccaaa gtgggaggcg gattcgcgga tccaccggtc gccaccatgg
     1801 tgagcaaggg cgaggaggat aacatggcca tcattaagga gttcatgcgc ttcaaggtgc
     1861 acatggaggg gtccgtgaac ggccacgtgt tcgagatcga gggcgagggc gagggccgcc
     1921 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttca
     1981 cctgggacat cctgagccct cagttcatgt acggctccaa tgcctacgtg aagcaccccg
     2041 ccgacatccc cgactacttt aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2101 tgaaattcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     2161 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc
     2221 agaagaagac catgggctgg gaggccctct ccgagcggat gtaccccgag gacggcgccc
     2281 tgaagggcga ggtcaagccc agagtgaagc tgaaggacgg cggccactac gacgctgagg
     2341 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaacc
     2401 gcaagctgga catcaccagc cacaacgagg actacaccat cgtggagcag tacgagagag
     2461 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc
     2521 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2581 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2641 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2701 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     2761 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2821 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2881 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2941 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3001 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3061 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3121 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3181 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3241 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3301 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3361 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3421 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3481 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3541 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3601 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3661 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3721 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3781 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3841 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3901 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3961 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4021 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4081 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4141 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4201 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4261 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4321 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4381 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4441 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4501 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4561 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4621 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4681 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4741 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4801 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4861 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4921 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4981 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5041 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5101 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5161 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5221 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5281 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5341 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5401 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5461 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5521 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5581 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5641 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5701 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5761 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5821 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     5881 tagcccatat atggagttcc g
//
