LOCUS       sequence-226465-       15471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-152
FEATURES             Location/Qualifiers
     source          1..15471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     110..131
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      160..254
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             298..957
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(364..383)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1022..1108
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1219..1384
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(1817..2767)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2815..3037
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     3720..3739
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            3777..3886
                     /note="incP origin of transfer"
     primer_bind     3909..3928
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4057..4848,4849..4917))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4680..4699
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4918..5009)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5028..5055)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5147..5233)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5386..5403
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12094..12378)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12152..12171)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12405..13283
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             13775..14569
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     GQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDVGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             join(14931..14933,14934..15446)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(14973..14994)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(15234..15253)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
       61 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      121 tggagttcgt gaccgccgcc gggtaattag ctgagcttgg actcctgttg atagatccag
      181 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
      241 ttattggtga gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg
      301 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      361 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      421 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      481 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag
      541 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      601 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      661 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
      721 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
      781 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
      841 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
      901 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt
      961 ttttaaggca gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca
     1021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1081 ggtgaacgct ctcctgagta ggacaaatcc gccgctagga gcttgcggcc gccaggggat
     1141 ctgattaatt aaaacgtggc ttactaggat ccgatatcat ttaaatctag ggataacagg
     1201 gtaatactag tgtcgaccaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     1261 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     1321 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     1381 cacaggctga aagcgctatt tcttccagaa ttgccatgat tttttcccca cgggaggcgt
     1441 cactggctcc cgtgttgtcg gcagctttga ttcgataagc agcatcgcct gtttcaggct
     1501 gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg tgttcaattt catgttctag
     1561 ttgctttgtt ttactggttt cacctgttct attaggtgtt acatgctgtt catctgttac
     1621 attgtcgatc tgttcatggt gaacagcttt aaatgcacca aaaactcgta aaagctctga
     1681 tgtatctatc ttttttacac cgttttcatc tgtgcatatg gacagttttc cctttgatat
     1741 gtaacggtga acagttgttc tacttttgtt tgttagtctt gatgcttcac tgatagatac
     1801 aagagccata agaacctcag atccttccgt atttagccag tatgttctct agtgtggttc
     1861 gttgtttttg cgtgagccat gagaacgaac cattgagatc atacttactt tgcatgtcac
     1921 tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg
     1981 tttttcttag tccgttatgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac
     2041 cattcatttt tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt
     2101 caacttggaa aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat
     2161 tgctgtaagt gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa
     2221 actcatggta gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta
     2281 tatttgcctt gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctca
     2341 tagagtattt gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta
     2401 actggaaaag ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga
     2461 acttggcata gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt
     2521 ccacagttct cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc
     2581 tactgatgtt catcatctga acgtattggt tataagtgaa cgataccgtc cgttctttcc
     2641 ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt
     2701 catgctccgt taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt
     2761 cagacataca tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta
     2821 gtcaatgata attactagtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga
     2881 cctttgctgg aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt
     2941 gtgttttttt tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa
     3001 agataaaaag aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt
     3061 attacaaaag gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta
     3121 aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg
     3181 accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct
     3241 ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa
     3301 ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg
     3361 ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc
     3421 tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc
     3481 agcggtatca tcaacaggct tacccgtctt actgtcgggg atcgacgctc tcccttatgc
     3541 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     3601 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     3661 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     3721 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccgccgggc
     3781 aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg
     3841 cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggcggc gaacgtggcg
     3901 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     3961 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaatc
     4021 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4081 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4141 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4201 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4261 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4321 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     4381 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4441 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4501 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4561 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4621 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     4681 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4741 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4801 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4861 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4921 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4981 atttgaatgt atttagaaaa ataaacaaaa gagtttgtag aaacgcaaaa aggccatccg
     5041 tcaggatggc cttctgctta atttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     5101 accctccggg ccgttgcttc gcaacgttca aatccgctcc cggcggattt gtcctactca
     5161 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     5221 ttcgttttat ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca
     5281 tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta
     5341 ctgccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc
     5401 aggctgaaaa tcttctctca tccgccaaaa cagccaagct tgcatgcctg caggtcgact
     5461 ctagaggatc ccctaaaaat aatgaccgag ctagctctaa ctgtgggatg tgataacgag
     5521 tcttgctata tggtgtttca gcttcgtaaa taaatgaact ctgcgctcca tccttgttgc
     5581 gtacggaagc aaaggacgag agattaggaa aatcgattat tttggctcgt tgaagatccg
     5641 atgcttcaaa ctcaatcaca cgatccgcct tatttatttg ctttgttgta ctgttaacca
     5701 ccttagctcg acttaataaa ggcaaatgag taaatcgagt ccatttccgt tcttgcattg
     5761 gattaaacca aacaaaaata cgccactcct tatgacccga gttacggaat aaatgaccaa
     5821 tatgtacaac ctgcacattg tcattaaatg tagctagcct aaccactcac tcttccccat
     5881 aaaccatttc caagaatctt tgtgcttcct cagttatccg ctctttagat aacgttgcac
     5941 ttcttaataa tcgccagcgc ctaagctcaa catcttcctg tttaagctta ataaaagctt
     6001 ggctaatccg tctaatttga taatcttcca cactctctga gtaacgcttt aagcaaagcg
     6061 ctaccaaagg cagtttctgt agattttttg caagagaggt tccgttagga gtttgcttta
     6121 acagccagct cgatgtcgct cttgggtgat caaggctact atctagacgc tttatgattc
     6181 ttaatagttg tcgtacagca attcgatctc tttggttcca atcaactcta ggggcgggtg
     6241 ccagacgctc ttgttgatgc tgttgattcc agtgaactag ccaatccctg tcatgtcggt
     6301 aaagccaagc gtatagcacc ccaccctcta aagactgtct tgccgcctta attccttggt
     6361 atttatgcac tagttgctgc cagtctttat gcttaacaga taaatcttca gagttaggtt
     6421 gcacagactg gctaacaggt cttgtcgtta tagagtgctc agtaagagca cttgcctgct
     6481 gtagcgcttc tataaccgtt agttttggca ataaggcttg ccacacaata ctatgctgta
     6541 aataactaaa ggcttttcta tgcttacgga atatactttt cagccaacac gtgtccttgt
     6601 tctctgccaa ctttaaatcc agtttctcta gtgcctcatc actaaaagtc tgcctcactc
     6661 tctccgccac caagtcatga cgaatgtgct tgcttttggt tagccctaga tcctgcgcta
     6721 agcgctgata aaacagcgtc cactgctcaa ggcttgggga aagctcttgc gctcgtggag
     6781 catctaacag aggggctata taagcagcta gtgctgttaa ttgagatagg gagtctttgg
     6841 ggtagtctga aagcagctca gtatgaccca aagcccaaaa ttgatgtcgg tgatcatcta
     6901 cagctctatc aaagaagact aaagcaccgt gttttggaca atatggcaaa gcgggcaaat
     6961 accaatctcg ttgccaaaag gcttccccat acctatttag ctgaagagca acgcaatcag
     7021 ggcagtatct aaagcggtta tcgctcttaa ctctagaagc agcgactcct agcattaaat
     7081 gcaccgcacc ttgcgcttgg tactccatta accgaatagc ttcgtctcgg cgctccttgc
     7141 ctacaaacgg agcatataaa gggaataagg tatgctcata aataagctgc tgaacagcgt
     7201 aacgtcctgt ttgatgtaga tgtcttgcta tcacacctaa atgcgagggc agacctaagg
     7261 tagcgaccac cttgcggttg ccatacacct catccaacag ctgcttagga ctaacaatcc
     7321 cttgataaac gcctgcccgt gcaatagtgc tataaatcag ctcattcgag tacggaacag
     7381 gaaagtttct catggctaac ctgcttgttt aaataagctc gccatatcca ctattacccc
     7441 tttccctttt aaccgttcat gcatggtttt ttcaggttgg cgttgtgaat agatataacg
     7501 taaatccgta tcaggcaagc tatcccagtc gctgggcttg actaccttta tagccgaaac
     7561 ctttttactc ttggagggct tttctagttc tgacactacc gtttcgcctt ccatcaacca
     7621 ctgcaaaaca agaggcagta acttttgtct tgtcatcgtt ggattctggc taaacgcttt
     7681 tttaatagtg ggaattaaca ggcttgaatc gtaatcctct ttcagcatca gatataaatg
     7741 acgctgatct tcggtatcta actcttgaag ggctttttct tctggtgttt gttcttgtat
     7801 cgctgcgata tctagctgaa gttggattaa ccgtttatca atctcgggaa cgactagatc
     7861 agaataacga gcaatgcgtt ctgggatacc cgagcgtaat gcctctagca tggggtgcac
     7921 aggctttaac tcatcttgat acacttgccg caataaacca gcggtaatac gctcattgcc
     7981 taaagctagc gcacggagct gagcgagtac aaaaagtttt actacaatgt ccatcactcc
     8041 ttggcttagc tcataccaca catcacggac ctcatccgat aacagcgcat ctttgcgttg
     8101 taaaagctgt aattgccaaa gattatccgt aaaagcgatc cactcttgat tgggctttcc
     8161 acgttgcgtt tgttgtatag gatcccagaa tatagctcca aaccctgccc ctctacgtgc
     8221 agaccgcaaa tcagcctcaa aaatctctcg tgctttaggg gtaccaatca acatcactgg
     8281 tacgccaata atattcacca tcgtcacaaa aaagttcagc atctcttgag atccacccga
     8341 acgagagcgg cttaaatgct gaatttcatc aataaccaac aaccctaaag catgtgcatt
     8401 ggctatttgc gacatcaaag ccaacatggt ttctatacca tgacgtttta agccataacg
     8461 acgctcatag ttcgagccca aggctcgatc caacgctctg aaaaaattca agcagatttc
     8521 ttttagcgaa ccattatgcg agcagtctat tttcaaatac accacctgct ctacattgag
     8581 ttcacgatgg taaatcacct gaggatacgt ggctagaata cgatgaagag aggtcgtctt
     8641 cccactacca gaacaaccaa ttaacaataa gctttgtgcc gtagatcgtg cctcctcaaa
     8701 gcgaaatgtc tccaactctc ccgtttgaac acgctcataa ccattttgta aatgcttttg
     8761 taaatctcct gttttaggat ttctgcctac gtagccacct cgaatcatga ccgaaatacg
     8821 ctcacttagt agcaaatgcg tacctaatgg ctgaaaatag tcatctggaa tacgacaaat
     8881 ggtatgagct ctgataacac gggacttttg caagtcagag gaagtaagct gtaaagagga
     8941 tttcagtgat gcagcactat tcactgactc ttgtaatggt ggtaaggcct cgataaaagg
     9001 attatcacga taagcctcta cccccgtatc acgataaact gcttgaatcc gggtagcact
     9061 catgactcat ccttttctgg tggatcctga aataattcag gcacgtatgt gggtaggctg
     9121 taatcttctt gatcatccgc ttccactgcg ttgaaaggaa taactttagc ctcatcacct
     9181 gaagagcttg gcttcaaatg ctccgcacgt tttttacgct ccgaggtcac ggcttctttt
     9241 ttattagttt taatctgctt aatgcgtgtt gatttgggct cagtagtact gggcgttaac
     9301 ttattcgctt tctgaatggt ttgctgaata aacgcctcaa gctccctgcg tttagttaac
     9361 tcatcctgct tcgcattggc tttattgtgt ttttcttgtg cttgtatatc ccaaacctcc
     9421 caaaatgaga gacctttaaa ctgccgacta cgttccgtca gattacagcg ccaaaataca
     9481 cggctgccaa cttgcggaaa caaataaatc gtatcaacca gcactgggtc ataagccgct
     9541 tctaaatgtt gaggtctagc tatatcagtg ctccgctgca accaaccctc acgcagaatc
     9601 tctgaccccg agtaatacaa accccacaaa ttaacgccaa atgaagaaat agagaccttt
     9661 cggcgaggca gtaacgctac tcgcaactgc tcttgctcca cagcccttaa actacctgta
     9721 cgatgctgca taccccattg ccatagctgg acaggaatag acggtaaatc tgtaggaaaa
     9781 tcagcatctc gatcgtattt atccatcacc agatggttat ttctgaataa gatcgtacgc
     9841 aaaataattt gtgtgaactc aaataccgac agagatgcat ctaacctata gtctgtttca
     9901 ccatggcttt tgatccgact gccctctaca atgccaggtg caaaggactt aaactcggct
     9961 tgtagtgttc taaaagtgct ttccactatg cctttagcat cgccacgtct aggtggagca
    10021 ctttccactc gcacattaaa actagaaact aaggcttcga cctgatgact cattaattcg
    10081 ccacggtccg ctagcaacac atctggcaaa cctacacacg gccagtctga gctactaatc
    10141 tcaatatcat gctgggcaca aatggccgtt ttgtcagagc aagcatttac aaaagcctgc
    10201 atcgccacca cataagacgg attttcaaag ccgatataaa agcccgtgat catccgacta
    10261 aacacatcaa tcacaatgta aagcgttggt cttcctatga ttttttggcg atcatgatga
    10321 tccactaaat aaatatcagc aatcgtggca tcaatctcat aacgactccc agggcctaac
    10381 gcctgagaag tggctgtact acttaagggt cgtacgtctt ttttatatac ccctgcttta
    10441 actctagact ttaagcgctg agctttaggg tattctcgat cataaaaata acgaaactga
    10501 cgtagtgttg ggtaatcctc ttggggaatg cgaggaaaat actgagcaaa caagtccaca
    10561 aatcgtctat aggcaacggt ggtctttgta cctttttgat ttaacaggtg cttttctatg
    10621 gtcaacctaa aaaggcgttc aatctcgggc gttaccttgg ttccttcacc cttaccatat
    10681 tctctggctc ggccaatctt tgctgttcct gtcgctgaac gtctttcccc tggtgcaccg
    10741 ctgtttttgt agtcaggaat taatgcatta ggcgtttgac cacgctgcca gtaacggcgt
    10801 aacaacttat aaactgtagc cttagtaacc ttatgttctt ggaccacatg ctcaacgagt
    10861 tcgcttctga ctttagggtc gaaacgatcc ttactattaa taataggaag aatttttcga
    10921 taatcctcct cgcgcttctg gaagctgaca gaatctacag acggctcttc taaatcaaga
    10981 tgtacataag ggtcatcagt tctaactaag cggccttcat ctaaatactg cattagcaac
    11041 aactcagccc tagcttgtgg aactccttta tcagcgctta tttgcatcca gacaacttgg
    11101 ccatcctcta tagccaaaat gcgatacgga tcattatcaa atagcacaac ctcattaatt
    11161 tgccacatag cgcaactcct ccatattcac tacttggcta atacagagat ctgcacactt
    11221 atttgccctg aaagacttat aaatattgaa cttaataaaa ccatttgcgg ttaaggctcg
    11281 tatctcactc aatgttttgc ctaactccaa atcataagca atatcaacct gcttacagac
    11341 attgataatg ttttcatctc ctttttcttg caggatatgg gccaatgggg atagttgtgc
    11401 taaaagctcc gcagaaactt cttctgtttt cactgaataa agccattcaa tattttcttt
    11461 tactacggga tttatttctt tatcagtaaa aatgaaccaa ggaatttgct tttgctgcca
    11521 atagcgacgc tctagttcta gtttttctaa ggtacgctcg tcttgtaagg ctgctgcagg
    11581 tttgacttga atagcaaact gctcaaaagg accatctttg cagtccacta aaaaatcagt
    11641 agacataacc tgatctacac cacgaataac aggatgctta ataccactat ctattgcaat
    11701 ctgcctggta tcactaggta ataaggggaa ctgctcgcgt atatctagca cgctgctctc
    11761 ccactcaaga ctgagaaaaa cagcaagctc taagtcagat agcaaatgat ggactcgtcc
    11821 cgtcttatga gaataaatac ggtgggaacg acctgaagaa ggaacttctt gtactgttag
    11881 ccatggaata tagtctttac catgcccttg gccacgcccc tctttaatac gacgggcaat
    11941 ttgcacttca gaaaatgaag agtttgcttt agccataaca aaagtccagt atgctttttc
    12001 acagcataac tggactgatt tcagtttaca actattctgt ctagtttaag acttgggtac
    12061 cgagctcgaa ttcgctagcc caaaaaaacg ggtatggaga aacagtagag agttgcgata
    12121 aaaagcgtca ggtaggatcc gctaatctta tggataaaaa tgctatggca tagcaaagtg
    12181 tgacgccgtg caaataatca atgtggactt ttctgccgtg attatagaca cttttgttac
    12241 gcgtttttgt catggctttg gtcccgcttt gttacagaat gcttttaata agcggggtta
    12301 ccggtttggt tagcgagaag agccagtaaa agacgcagtg acggcaatgt ctgatgcaat
    12361 atggacaatt ggtttcttct ctgaatggcg ggagtatgaa aagtatggct gaagcgcaaa
    12421 atgatcccct gctgccggga tactcgttta atgcccatct ggtggcgggt ttaacgccga
    12481 ttgaggccaa cggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata
    12541 ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgttt
    12601 gccgaccggg tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc
    12661 cggaggctcg cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg
    12721 aatggcttaa ctggccgtca atatttgcca atacggggtt ctttcgcccg gatgaagcgc
    12781 accagccgca tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc
    12841 gctattcgga gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag
    12901 cgattaacga gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca
    12961 tcagcgatca cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgct
    13021 tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct
    13081 ggcgcgagga ccaacgtatc agccaggcga agctgctttt gagcaccacc cggatgccta
    13141 tcgccaccgt cggtcgcaat gttggttttg acgatcaact ctatttctcg cgggtattta
    13201 aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg
    13261 atgtagccgt caagttgtca taattggtaa cgaatcagac aattgacggc ttgacggagt
    13321 agcatagggt ttgcagaatc cctgcttcgt ccatttgaca ggcacattat gcatcgatga
    13381 taagctgtca aacatgagca gatcctctac gccggacgca tcgtggcctg tgggcggaca
    13441 ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct taaactagac
    13501 agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg
    13561 ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg
    13621 ggcgtggggt cgacgcggcc gtggcgcgcc tcctaggtgc tcgagtggcg gccgctattg
    13681 acccataaac tgccaggaat tggggatcgg attcgagcta agaattccct tggggtatct
    13741 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
    13801 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
    13861 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
    13921 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
    13981 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
    14041 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatggac aaagccctga
    14101 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
    14161 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
    14221 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
    14281 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
    14341 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
    14401 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
    14461 ggaggatgtc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
    14521 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttaga gctcgcacgc
    14581 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
    14641 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
    14701 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
    14761 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
    14821 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
    14881 aggggatcaa gatctgatca agagacagga tactagtgga ggaagaaaaa atgagcaagg
    14941 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
    15001 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
    15061 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
    15121 tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
    15181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
    15241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
    15301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
    15361 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
    15421 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac c
//
