LOCUS       sequence-226471-       15498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15498)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-150
FEATURES             Location/Qualifiers
     source          1..15498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..711
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      728..822
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             866..1525
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(932..951)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1590..1676
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1787..1952
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(2385..3335)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3383..3605
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     4288..4307
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            4345..4454
                     /note="incP origin of transfer"
     primer_bind     4477..4496
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4625..5416,5417..5485))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5248..5267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5486..5577)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5596..5623)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5715..5801)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5954..5971
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12662..12946)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12720..12739)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12973..13851
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             14343..15137
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     GQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDVGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
       61 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      121 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      181 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
      241 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      301 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
      361 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
      421 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
      481 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
      541 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
      601 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
      661 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttaattagct
      721 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      781 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgag
      841 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      901 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      961 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     1021 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     1081 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     1141 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     1201 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     1261 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     1321 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     1381 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     1441 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     1501 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     1561 tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact
     1621 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1681 cgctaggagc ttgcggccgc caggggatct gattaattaa aacgtggctt actaggatcc
     1741 gatatcattt aaatctaggg ataacagggt aatactagtg tcgaccaacc agataagtga
     1801 aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc
     1861 cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc
     1921 ctcccagttc ccaactattt tgtccgccca caggctgaaa gcgctatttc ttccagaatt
     1981 gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt
     2041 cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt
     2101 gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat
     2161 taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttaa
     2221 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     2281 tgcatatgga cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg
     2341 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     2401 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     2461 ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     2521 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct
     2581 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     2641 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     2701 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     2761 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     2821 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     2881 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     2941 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     3001 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     3061 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     3121 ttagctaata caccataagc attttcccta ctgatgttca tcatctgaac gtattggtta
     3181 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     3241 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     3301 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     3361 taatcactat accaattgag atgggctagt caatgataat tactagtcct tttcctttga
     3421 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     3481 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     3541 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     3601 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     3661 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     3721 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     3781 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     3841 gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg
     3901 cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca
     3961 gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat
     4021 tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac
     4081 tgtcggggat cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     4141 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     4201 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     4261 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     4321 acctgtggcg ccggtgatgc cgccgggcag gataggtgaa gtaggcccac ccgcgagcgg
     4381 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct
     4441 gcgaggctgg ccggcggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4501 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4561 taatgcgccg ctacagggcg cgtaaatcaa tctaaagtat atatgagtaa acttggtctg
     4621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4741 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4921 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5221 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     5281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5461 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5521 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaaaga
     5581 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg
     5641 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa
     5701 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     5761 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac
     5821 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
     5881 catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttatcagacc
     5941 gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca
     6001 gccaagcttg catgcctgca ggtcgactct agaggatccc ctaaaaataa tgaccgagct
     6061 agctctaact gtgggatgtg ataacgagtc ttgctatatg gtgtttcagc ttcgtaaata
     6121 aatgaactct gcgctccatc cttgttgcgt acggaagcaa aggacgagag attaggaaaa
     6181 tcgattattt tggctcgttg aagatccgat gcttcaaact caatcacacg atccgcctta
     6241 tttatttgct ttgttgtact gttaaccacc ttagctcgac ttaataaagg caaatgagta
     6301 aatcgagtcc atttccgttc ttgcattgga ttaaaccaaa caaaaatacg ccactcctta
     6361 tgacccgagt tacggaataa atgaccaata tgtacaacct gcacattgtc attaaatgta
     6421 gctagcctaa ccactcactc ttccccataa accatttcca agaatctttg tgcttcctca
     6481 gttatccgct ctttagataa cgttgcactt cttaataatc gccagcgcct aagctcaaca
     6541 tcttcctgtt taagcttaat aaaagcttgg ctaatccgtc taatttgata atcttccaca
     6601 ctctctgagt aacgctttaa gcaaagcgct accaaaggca gtttctgtag attttttgca
     6661 agagaggttc cgttaggagt ttgctttaac agccagctcg atgtcgctct tgggtgatca
     6721 aggctactat ctagacgctt tatgattctt aatagttgtc gtacagcaat tcgatctctt
     6781 tggttccaat caactctagg ggcgggtgcc agacgctctt gttgatgctg ttgattccag
     6841 tgaactagcc aatccctgtc atgtcggtaa agccaagcgt atagcacccc accctctaaa
     6901 gactgtcttg ccgccttaat tccttggtat ttatgcacta gttgctgcca gtctttatgc
     6961 ttaacagata aatcttcaga gttaggttgc acagactggc taacaggtct tgtcgttata
     7021 gagtgctcag taagagcact tgcctgctgt agcgcttcta taaccgttag ttttggcaat
     7081 aaggcttgcc acacaatact atgctgtaaa taactaaagg cttttctatg cttacggaat
     7141 atacttttca gccaacacgt gtccttgttc tctgccaact ttaaatccag tttctctagt
     7201 gcctcatcac taaaagtctg cctcactctc tccgccacca agtcatgacg aatgtgcttg
     7261 cttttggtta gccctagatc ctgcgctaag cgctgataaa acagcgtcca ctgctcaagg
     7321 cttggggaaa gctcttgcgc tcgtggagca tctaacagag gggctatata agcagctagt
     7381 gctgttaatt gagataggga gtctttgggg tagtctgaaa gcagctcagt atgacccaaa
     7441 gcccaaaatt gatgtcggtg atcatctaca gctctatcaa agaagactaa agcaccgtgt
     7501 tttggacaat atggcaaagc gggcaaatac caatctcgtt gccaaaaggc ttccccatac
     7561 ctatttagct gaagagcaac gcaatcaggg cagtatctaa agcggttatc gctcttaact
     7621 ctagaagcag cgactcctag cattaaatgc accgcacctt gcgcttggta ctccattaac
     7681 cgaatagctt cgtctcggcg ctccttgcct acaaacggag catataaagg gaataaggta
     7741 tgctcataaa taagctgctg aacagcgtaa cgtcctgttt gatgtagatg tcttgctatc
     7801 acacctaaat gcgagggcag acctaaggta gcgaccacct tgcggttgcc atacacctca
     7861 tccaacagct gcttaggact aacaatccct tgataaacgc ctgcccgtgc aatagtgcta
     7921 taaatcagct cattcgagta cggaacagga aagtttctca tggctaacct gcttgtttaa
     7981 ataagctcgc catatccact attacccctt tcccttttaa ccgttcatgc atggtttttt
     8041 caggttggcg ttgtgaatag atataacgta aatccgtatc aggcaagcta tcccagtcgc
     8101 tgggcttgac tacctttata gccgaaacct ttttactctt ggagggcttt tctagttctg
     8161 acactaccgt ttcgccttcc atcaaccact gcaaaacaag aggcagtaac ttttgtcttg
     8221 tcatcgttgg attctggcta aacgcttttt taatagtggg aattaacagg cttgaatcgt
     8281 aatcctcttt cagcatcaga tataaatgac gctgatcttc ggtatctaac tcttgaaggg
     8341 ctttttcttc tggtgtttgt tcttgtatcg ctgcgatatc tagctgaagt tggattaacc
     8401 gtttatcaat ctcgggaacg actagatcag aataacgagc aatgcgttct gggatacccg
     8461 agcgtaatgc ctctagcatg gggtgcacag gctttaactc atcttgatac acttgccgca
     8521 ataaaccagc ggtaatacgc tcattgccta aagctagcgc acggagctga gcgagtacaa
     8581 aaagttttac tacaatgtcc atcactcctt ggcttagctc ataccacaca tcacggacct
     8641 catccgataa cagcgcatct ttgcgttgta aaagctgtaa ttgccaaaga ttatccgtaa
     8701 aagcgatcca ctcttgattg ggctttccac gttgcgtttg ttgtatagga tcccagaata
     8761 tagctccaaa ccctgcccct ctacgtgcag accgcaaatc agcctcaaaa atctctcgtg
     8821 ctttaggggt accaatcaac atcactggta cgccaataat attcaccatc gtcacaaaaa
     8881 agttcagcat ctcttgagat ccacccgaac gagagcggct taaatgctga atttcatcaa
     8941 taaccaacaa ccctaaagca tgtgcattgg ctatttgcga catcaaagcc aacatggttt
     9001 ctataccatg acgttttaag ccataacgac gctcatagtt cgagcccaag gctcgatcca
     9061 acgctctgaa aaaattcaag cagatttctt ttagcgaacc attatgcgag cagtctattt
     9121 tcaaatacac cacctgctct acattgagtt cacgatggta aatcacctga ggatacgtgg
     9181 ctagaatacg atgaagagag gtcgtcttcc cactaccaga acaaccaatt aacaataagc
     9241 tttgtgccgt agatcgtgcc tcctcaaagc gaaatgtctc caactctccc gtttgaacac
     9301 gctcataacc attttgtaaa tgcttttgta aatctcctgt tttaggattt ctgcctacgt
     9361 agccacctcg aatcatgacc gaaatacgct cacttagtag caaatgcgta cctaatggct
     9421 gaaaatagtc atctggaata cgacaaatgg tatgagctct gataacacgg gacttttgca
     9481 agtcagagga agtaagctgt aaagaggatt tcagtgatgc agcactattc actgactctt
     9541 gtaatggtgg taaggcctcg ataaaaggat tatcacgata agcctctacc cccgtatcac
     9601 gataaactgc ttgaatccgg gtagcactca tgactcatcc ttttctggtg gatcctgaaa
     9661 taattcaggc acgtatgtgg gtaggctgta atcttcttga tcatccgctt ccactgcgtt
     9721 gaaaggaata actttagcct catcacctga agagcttggc ttcaaatgct ccgcacgttt
     9781 tttacgctcc gaggtcacgg cttctttttt attagtttta atctgcttaa tgcgtgttga
     9841 tttgggctca gtagtactgg gcgttaactt attcgctttc tgaatggttt gctgaataaa
     9901 cgcctcaagc tccctgcgtt tagttaactc atcctgcttc gcattggctt tattgtgttt
     9961 ttcttgtgct tgtatatccc aaacctccca aaatgagaga cctttaaact gccgactacg
    10021 ttccgtcaga ttacagcgcc aaaatacacg gctgccaact tgcggaaaca aataaatcgt
    10081 atcaaccagc actgggtcat aagccgcttc taaatgttga ggtctagcta tatcagtgct
    10141 ccgctgcaac caaccctcac gcagaatctc tgaccccgag taatacaaac cccacaaatt
    10201 aacgccaaat gaagaaatag agacctttcg gcgaggcagt aacgctactc gcaactgctc
    10261 ttgctccaca gcccttaaac tacctgtacg atgctgcata ccccattgcc atagctggac
    10321 aggaatagac ggtaaatctg taggaaaatc agcatctcga tcgtatttat ccatcaccag
    10381 atggttattt ctgaataaga tcgtacgcaa aataatttgt gtgaactcaa ataccgacag
    10441 agatgcatct aacctatagt ctgtttcacc atggcttttg atccgactgc cctctacaat
    10501 gccaggtgca aaggacttaa actcggcttg tagtgttcta aaagtgcttt ccactatgcc
    10561 tttagcatcg ccacgtctag gtggagcact ttccactcgc acattaaaac tagaaactaa
    10621 ggcttcgacc tgatgactca ttaattcgcc acggtccgct agcaacacat ctggcaaacc
    10681 tacacacggc cagtctgagc tactaatctc aatatcatgc tgggcacaaa tggccgtttt
    10741 gtcagagcaa gcatttacaa aagcctgcat cgccaccaca taagacggat tttcaaagcc
    10801 gatataaaag cccgtgatca tccgactaaa cacatcaatc acaatgtaaa gcgttggtct
    10861 tcctatgatt ttttggcgat catgatgatc cactaaataa atatcagcaa tcgtggcatc
    10921 aatctcataa cgactcccag ggcctaacgc ctgagaagtg gctgtactac ttaagggtcg
    10981 tacgtctttt ttatataccc ctgctttaac tctagacttt aagcgctgag ctttagggta
    11041 ttctcgatca taaaaataac gaaactgacg tagtgttggg taatcctctt ggggaatgcg
    11101 aggaaaatac tgagcaaaca agtccacaaa tcgtctatag gcaacggtgg tctttgtacc
    11161 tttttgattt aacaggtgct tttctatggt caacctaaaa aggcgttcaa tctcgggcgt
    11221 taccttggtt ccttcaccct taccatattc tctggctcgg ccaatctttg ctgttcctgt
    11281 cgctgaacgt ctttcccctg gtgcaccgct gtttttgtag tcaggaatta atgcattagg
    11341 cgtttgacca cgctgccagt aacggcgtaa caacttataa actgtagcct tagtaacctt
    11401 atgttcttgg accacatgct caacgagttc gcttctgact ttagggtcga aacgatcctt
    11461 actattaata ataggaagaa tttttcgata atcctcctcg cgcttctgga agctgacaga
    11521 atctacagac ggctcttcta aatcaagatg tacataaggg tcatcagttc taactaagcg
    11581 gccttcatct aaatactgca ttagcaacaa ctcagcccta gcttgtggaa ctcctttatc
    11641 agcgcttatt tgcatccaga caacttggcc atcctctata gccaaaatgc gatacggatc
    11701 attatcaaat agcacaacct cattaatttg ccacatagcg caactcctcc atattcacta
    11761 cttggctaat acagagatct gcacacttat ttgccctgaa agacttataa atattgaact
    11821 taataaaacc atttgcggtt aaggctcgta tctcactcaa tgttttgcct aactccaaat
    11881 cataagcaat atcaacctgc ttacagacat tgataatgtt ttcatctcct ttttcttgca
    11941 ggatatgggc caatggggat agttgtgcta aaagctccgc agaaacttct tctgttttca
    12001 ctgaataaag ccattcaata ttttctttta ctacgggatt tatttcttta tcagtaaaaa
    12061 tgaaccaagg aatttgcttt tgctgccaat agcgacgctc tagttctagt ttttctaagg
    12121 tacgctcgtc ttgtaaggct gctgcaggtt tgacttgaat agcaaactgc tcaaaaggac
    12181 catctttgca gtccactaaa aaatcagtag acataacctg atctacacca cgaataacag
    12241 gatgcttaat accactatct attgcaatct gcctggtatc actaggtaat aaggggaact
    12301 gctcgcgtat atctagcacg ctgctctccc actcaagact gagaaaaaca gcaagctcta
    12361 agtcagatag caaatgatgg actcgtcccg tcttatgaga ataaatacgg tgggaacgac
    12421 ctgaagaagg aacttcttgt actgttagcc atggaatata gtctttacca tgcccttggc
    12481 cacgcccctc tttaatacga cgggcaattt gcacttcaga aaatgaagag tttgctttag
    12541 ccataacaaa agtccagtat gctttttcac agcataactg gactgatttc agtttacaac
    12601 tattctgtct agtttaagac ttgggtaccg agctcgaatt cgctagccca aaaaaacggg
    12661 tatggagaaa cagtagagag ttgcgataaa aagcgtcagg taggatccgc taatcttatg
    12721 gataaaaatg ctatggcata gcaaagtgtg acgccgtgca aataatcaat gtggactttt
    12781 ctgccgtgat tatagacact tttgttacgc gtttttgtca tggctttggt cccgctttgt
    12841 tacagaatgc ttttaataag cggggttacc ggtttggtta gcgagaagag ccagtaaaag
    12901 acgcagtgac ggcaatgtct gatgcaatat ggacaattgg tttcttctct gaatggcggg
    12961 agtatgaaaa gtatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaat
    13021 gcccatctgg tggcgggttt aacgccgatt gaggccaacg gttatctcga tttttttatc
    13081 gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg
    13141 gtgaaaaatc agggacgaga atttgtttgc cgaccgggtg atattttgct gttcccgcca
    13201 ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt
    13261 tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat
    13321 acggggttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa
    13381 atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt
    13441 gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat
    13501 aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat
    13561 atcgccagcg tcgcacagca tgtttgcttg tcgccgtcgc gtctgtcaca tcttttccgc
    13621 cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgtatcag ccaggcgaag
    13681 ctgcttttga gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac
    13741 gatcaactct atttctcgcg ggtatttaaa aaatgcaccg gggccagccc gagcgagttc
    13801 cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcata attggtaacg
    13861 aatcagacaa ttgacggctt gacggagtag catagggttt gcagaatccc tgcttcgtcc
    13921 atttgacagg cacattatgc atcgatgata agctgtcaaa catgagcaga tcctctacgc
    13981 cggacgcatc gtggcctgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa
    14041 actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta
    14101 tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa
    14161 gtgcaaattg cccgtcgtat taaagagggg cgtggggtcg acgcggccgt ggcgcgcctc
    14221 ctaggtgctc gagtggcggc cgctattgac ccataaactg ccaggaattg gggatcggat
    14281 tcgagctaag aattcccttg gggtatcttt aaatactgta gaaaagagga aggaaataat
    14341 aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
    14401 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
    14461 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
    14521 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
    14581 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
    14641 aagagtatga agatggacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
    14701 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
    14761 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
    14821 aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
    14881 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
    14941 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
    15001 acattgcctt ctgcgtccgg tcgatcaggg aggatgtcgg ggaagaacag tatgtcgagc
    15061 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
    15121 tggatgaatt gttttagagc tcgcacgctg ccgcaagcac tcagggcgca agggctgcta
    15181 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
    15241 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
    15301 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
    15361 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
    15421 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggata
    15481 ctagtggagg aagaaaaa
//
