LOCUS       sequence-226481-        5249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses araC constitutively. Expresses alpha-hemolysin and 
            mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Dupin A, Simmel FC
  TITLE     Signalling and differentiation in emulsion-based 
            multi-compartmentalized in vitro gene circuits.
  JOURNAL   Nat Chem. 2018 Nov 26. pii: 10.1038/s41557-018-0174-9. doi: 
            10.1038/s41557-018-0174-9.
  PUBMED    30478365
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem. 
            2018 Nov 26. pii: 10.1038/s41557-018-0174-9. doi: 
            10.1038/s41557-018-0174-9."
COMMENT     Sequence Label: pSB1A3-AD010
FEATURES             Location/Qualifiers
     source          1..5249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(73..177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     245..263
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(267..310)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    313..334
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(420..1298)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1325..1609
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1532..1551
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             2704..2715
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    3505..3525
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(3531..3548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3702..4290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3782..3801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4461..5249,1..3,4..72))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5084..5103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
       61 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      181 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      241 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
      301 ggatgatttc tggaattcgc ggccgcttct agaggagagt taggcataag gctgcatgct
      361 accctttggt cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct
      421 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc
      481 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca
      541 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg
      601 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
      661 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
      721 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
      781 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
      841 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
      901 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat
      961 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
     1021 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
     1081 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
     1141 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
     1201 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
     1261 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
     1321 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
     1381 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
     1441 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
     1501 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
     1561 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
     1621 tgggctagcg aattcgagct cggtacccgg ggatcctcta gaaggagata taaccctatg
     1681 gactatgttt ttcaaagacg aaaaactact agatggattc tgatatcaat atcaaaaccg
     1741 gcaccaccga tatcggctcc aataccaccg ttaaaaccgg tgatctggtg acctatgata
     1801 aagaaaacgg tatgcataaa aaagtgtttt actcgtttat tgacgataaa aaccataaca
     1861 aaaaactgct ggtcatccgc accaaaggca ccattgcggg tcaataccgt gtgtactccg
     1921 aagaaggtgc gaacaaaagc ggtctggctt ggccgtctgc ctttaaagtg cagctgcaac
     1981 tgccggataa tgaagtggcg cagatttcag attattatcc gcgtaatagc atcgatacca
     2041 aagaatatat gagtaccctg acctatggtt ttaatggcaa tgttaccggt gatgatacgg
     2101 gtaaaattgg cggtctgatt ggcgccaatg tgtccattgg tcatacgctg aaatacgtgc
     2161 aaccggattt caaaaccatt ctggaaagtc cgaccgataa aaaagtgggt tggaaagtta
     2221 tcttcaacaa catggtgaat cagaactggg gtccgtacga tcgcgattcc tggaatccgg
     2281 tttatggcaa tcagctgttt atgaaaaccc gcaacggtag tatgaaagcg gcggataatt
     2341 ttctggaccc gaacaaagcc tcaagcctgc tgtccagcgg ttttagcccg gattttgcca
     2401 cggttattac catggatcgc aaagccagca aacagcagac caacattgat gtgatctacg
     2461 aacgtgtgcg tgatgattat caactgcatt ggacctcaac caattggaaa ggcaccaata
     2521 ccaaagataa atggacggat cgcagttcag aacgctacaa aattgattgg gaaaaagaag
     2581 aaatgaccaa ctaaagcgct caacgggtgt gcttcccgtt ctgatgagtc cgtgaggacg
     2641 aaagcgcctc tacaaataat tttgtttaag gagctatgga ctatgtttga aaggctgctc
     2701 gagaaagagg agaaaatggt gtcaaaggga gaggcggtta tcaaggaatt tatgcgcttt
     2761 aaagtccaca tggagggcag catgaacggg cacgagtttg aaattgaggg ggagggggag
     2821 ggccgtcctt atgaaggtac tcagactgct aaactgaagg tgacaaaagg tggccccttg
     2881 cctttctcgt gggacatcct gtcgccacaa ttcatgtacg ggagccgcgc ctttatcaaa
     2941 catcccgcag atattcctga ttactataaa caatctttcc cggaaggttt caaatgggaa
     3001 cgcgtcatga attttgagga cgggggcgct gtcacagtta ctcaggacac ctccttggaa
     3061 gacggcacat tgatttacaa ggttaagttg cgcggcacaa acttcccccc tgacgggcca
     3121 gtaatgcaaa agaaaactat gggttgggag gcgtctacag aacgtttata ccccgaagac
     3181 ggggtgctga aaggtgacat taagatggcc ctgcgcctga aggacggcgg tcgctatctt
     3241 gccgacttta aaactactta taaggctaaa aaaccagtcc agatgccagg cgcctataat
     3301 gttgaccgca agttagacat cacctcacat aatgaagact ataccgttgt agaacaatac
     3361 gagcgcagcg agggtcgtca cagtaccggg gggatggatg aattatacaa ataaaagctt
     3421 tagaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgccttg
     3481 tcacacctac actgctcgaa gtactactag tagcggccgc tgcaggcttc ctcgctcact
     3541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
     3721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcattagatt atcaaaaagg
     4381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5221 gcatctttta ctttcaccag cgtttctgg
//
