LOCUS       sequence-226484-       14648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14648)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 14648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-131
FEATURES             Location/Qualifiers
     source          1..14648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..717)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(43..64)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(304..323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     651..672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      731..825
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             869..1528
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(935..954)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1593..1679
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1790..1955
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(2388..3338)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3386..3608
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     4291..4310
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            4348..4457
                     /note="incP origin of transfer"
     primer_bind     4480..4499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4628..5419,5420..5488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5251..5270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5489..5580)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5599..5626)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5718..5804)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5957..5974
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12665..12949)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12723..12742)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12976..13854
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 atgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
       61 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      181 gtgaccaccc tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
      241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      421 ctggagtaca actactttag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
      481 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
      541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      601 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      661 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta
      721 gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      781 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagcttggc
      841 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      901 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      961 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     1021 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     1081 ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     1141 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     1201 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     1261 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     1321 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     1381 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     1441 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     1501 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
     1561 gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag
     1621 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     1681 cgccgctagg agcttgcggc cgccagggga tctgattaat taaaacgtgg cttactagga
     1741 tccgatatca tttaaatcta gggataacag ggtaatacta gtgtcgacca accagataag
     1801 tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac
     1861 acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca
     1921 cccctcccag ttcccaacta ttttgtccgc ccacaggctg aaagcgctat ttcttccaga
     1981 attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg
     2041 attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa
     2101 gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc
     2161 tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt
     2221 taaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat
     2281 ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt
     2341 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg
     2401 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa
     2461 ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc
     2521 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa
     2581 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt
     2641 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg
     2701 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt
     2761 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac
     2821 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt
     2881 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca
     2941 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
     3001 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
     3061 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
     3121 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg aacgtattgg
     3181 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
     3241 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
     3301 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
     3361 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt
     3421 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
     3481 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
     3541 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
     3601 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
     3661 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
     3721 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
     3781 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
     3841 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
     3901 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
     3961 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
     4021 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
     4081 tactgtcggg gatcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     4141 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     4201 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     4261 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     4321 cgcacctgtg gcgccggtga tgccgccggg caggataggt gaagtaggcc cacccgcgag
     4381 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
     4441 tctgcgaggc tggccggcgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4501 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4561 gcttaatgcg ccgctacagg gcgcgtaaat caatctaaag tatatatgag taaacttggt
     4621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4921 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5221 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     5281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5581 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc
     5641 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
     5701 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     5761 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     5821 tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt
     5881 cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaattct gttttatcag
     5941 accgcttctg cgttctgatt taatctgtat caggctgaaa atcttctctc atccgccaaa
     6001 acagccaagc ttgcatgcct gcaggtcgac tctagaggat cccctaaaaa taatgaccga
     6061 gctagctcta actgtgggat gtgataacga gtcttgctat atggtgtttc agcttcgtaa
     6121 ataaatgaac tctgcgctcc atccttgttg cgtacggaag caaaggacga gagattagga
     6181 aaatcgatta ttttggctcg ttgaagatcc gatgcttcaa actcaatcac acgatccgcc
     6241 ttatttattt gctttgttgt actgttaacc accttagctc gacttaataa aggcaaatga
     6301 gtaaatcgag tccatttccg ttcttgcatt ggattaaacc aaacaaaaat acgccactcc
     6361 ttatgacccg agttacggaa taaatgacca atatgtacaa cctgcacatt gtcattaaat
     6421 gtagctagcc taaccactca ctcttcccca taaaccattt ccaagaatct ttgtgcttcc
     6481 tcagttatcc gctctttaga taacgttgca cttcttaata atcgccagcg cctaagctca
     6541 acatcttcct gtttaagctt aataaaagct tggctaatcc gtctaatttg ataatcttcc
     6601 acactctctg agtaacgctt taagcaaagc gctaccaaag gcagtttctg tagatttttt
     6661 gcaagagagg ttccgttagg agtttgcttt aacagccagc tcgatgtcgc tcttgggtga
     6721 tcaaggctac tatctagacg ctttatgatt cttaatagtt gtcgtacagc aattcgatct
     6781 ctttggttcc aatcaactct aggggcgggt gccagacgct cttgttgatg ctgttgattc
     6841 cagtgaacta gccaatccct gtcatgtcgg taaagccaag cgtatagcac cccaccctct
     6901 aaagactgtc ttgccgcctt aattccttgg tatttatgca ctagttgctg ccagtcttta
     6961 tgcttaacag ataaatcttc agagttaggt tgcacagact ggctaacagg tcttgtcgtt
     7021 atagagtgct cagtaagagc acttgcctgc tgtagcgctt ctataaccgt tagttttggc
     7081 aataaggctt gccacacaat actatgctgt aaataactaa aggcttttct atgcttacgg
     7141 aatatacttt tcagccaaca cgtgtccttg ttctctgcca actttaaatc cagtttctct
     7201 agtgcctcat cactaaaagt ctgcctcact ctctccgcca ccaagtcatg acgaatgtgc
     7261 ttgcttttgg ttagccctag atcctgcgct aagcgctgat aaaacagcgt ccactgctca
     7321 aggcttgggg aaagctcttg cgctcgtgga gcatctaaca gaggggctat ataagcagct
     7381 agtgctgtta attgagatag ggagtctttg gggtagtctg aaagcagctc agtatgaccc
     7441 aaagcccaaa attgatgtcg gtgatcatct acagctctat caaagaagac taaagcaccg
     7501 tgttttggac aatatggcaa agcgggcaaa taccaatctc gttgccaaaa ggcttcccca
     7561 tacctattta gctgaagagc aacgcaatca gggcagtatc taaagcggtt atcgctctta
     7621 actctagaag cagcgactcc tagcattaaa tgcaccgcac cttgcgcttg gtactccatt
     7681 aaccgaatag cttcgtctcg gcgctccttg cctacaaacg gagcatataa agggaataag
     7741 gtatgctcat aaataagctg ctgaacagcg taacgtcctg tttgatgtag atgtcttgct
     7801 atcacaccta aatgcgaggg cagacctaag gtagcgacca ccttgcggtt gccatacacc
     7861 tcatccaaca gctgcttagg actaacaatc ccttgataaa cgcctgcccg tgcaatagtg
     7921 ctataaatca gctcattcga gtacggaaca ggaaagtttc tcatggctaa cctgcttgtt
     7981 taaataagct cgccatatcc actattaccc ctttcccttt taaccgttca tgcatggttt
     8041 tttcaggttg gcgttgtgaa tagatataac gtaaatccgt atcaggcaag ctatcccagt
     8101 cgctgggctt gactaccttt atagccgaaa cctttttact cttggagggc ttttctagtt
     8161 ctgacactac cgtttcgcct tccatcaacc actgcaaaac aagaggcagt aacttttgtc
     8221 ttgtcatcgt tggattctgg ctaaacgctt ttttaatagt gggaattaac aggcttgaat
     8281 cgtaatcctc tttcagcatc agatataaat gacgctgatc ttcggtatct aactcttgaa
     8341 gggctttttc ttctggtgtt tgttcttgta tcgctgcgat atctagctga agttggatta
     8401 accgtttatc aatctcggga acgactagat cagaataacg agcaatgcgt tctgggatac
     8461 ccgagcgtaa tgcctctagc atggggtgca caggctttaa ctcatcttga tacacttgcc
     8521 gcaataaacc agcggtaata cgctcattgc ctaaagctag cgcacggagc tgagcgagta
     8581 caaaaagttt tactacaatg tccatcactc cttggcttag ctcataccac acatcacgga
     8641 cctcatccga taacagcgca tctttgcgtt gtaaaagctg taattgccaa agattatccg
     8701 taaaagcgat ccactcttga ttgggctttc cacgttgcgt ttgttgtata ggatcccaga
     8761 atatagctcc aaaccctgcc cctctacgtg cagaccgcaa atcagcctca aaaatctctc
     8821 gtgctttagg ggtaccaatc aacatcactg gtacgccaat aatattcacc atcgtcacaa
     8881 aaaagttcag catctcttga gatccacccg aacgagagcg gcttaaatgc tgaatttcat
     8941 caataaccaa caaccctaaa gcatgtgcat tggctatttg cgacatcaaa gccaacatgg
     9001 tttctatacc atgacgtttt aagccataac gacgctcata gttcgagccc aaggctcgat
     9061 ccaacgctct gaaaaaattc aagcagattt cttttagcga accattatgc gagcagtcta
     9121 ttttcaaata caccacctgc tctacattga gttcacgatg gtaaatcacc tgaggatacg
     9181 tggctagaat acgatgaaga gaggtcgtct tcccactacc agaacaacca attaacaata
     9241 agctttgtgc cgtagatcgt gcctcctcaa agcgaaatgt ctccaactct cccgtttgaa
     9301 cacgctcata accattttgt aaatgctttt gtaaatctcc tgttttagga tttctgccta
     9361 cgtagccacc tcgaatcatg accgaaatac gctcacttag tagcaaatgc gtacctaatg
     9421 gctgaaaata gtcatctgga atacgacaaa tggtatgagc tctgataaca cgggactttt
     9481 gcaagtcaga ggaagtaagc tgtaaagagg atttcagtga tgcagcacta ttcactgact
     9541 cttgtaatgg tggtaaggcc tcgataaaag gattatcacg ataagcctct acccccgtat
     9601 cacgataaac tgcttgaatc cgggtagcac tcatgactca tccttttctg gtggatcctg
     9661 aaataattca ggcacgtatg tgggtaggct gtaatcttct tgatcatccg cttccactgc
     9721 gttgaaagga ataactttag cctcatcacc tgaagagctt ggcttcaaat gctccgcacg
     9781 ttttttacgc tccgaggtca cggcttcttt tttattagtt ttaatctgct taatgcgtgt
     9841 tgatttgggc tcagtagtac tgggcgttaa cttattcgct ttctgaatgg tttgctgaat
     9901 aaacgcctca agctccctgc gtttagttaa ctcatcctgc ttcgcattgg ctttattgtg
     9961 tttttcttgt gcttgtatat cccaaacctc ccaaaatgag agacctttaa actgccgact
    10021 acgttccgtc agattacagc gccaaaatac acggctgcca acttgcggaa acaaataaat
    10081 cgtatcaacc agcactgggt cataagccgc ttctaaatgt tgaggtctag ctatatcagt
    10141 gctccgctgc aaccaaccct cacgcagaat ctctgacccc gagtaataca aaccccacaa
    10201 attaacgcca aatgaagaaa tagagacctt tcggcgaggc agtaacgcta ctcgcaactg
    10261 ctcttgctcc acagccctta aactacctgt acgatgctgc ataccccatt gccatagctg
    10321 gacaggaata gacggtaaat ctgtaggaaa atcagcatct cgatcgtatt tatccatcac
    10381 cagatggtta tttctgaata agatcgtacg caaaataatt tgtgtgaact caaataccga
    10441 cagagatgca tctaacctat agtctgtttc accatggctt ttgatccgac tgccctctac
    10501 aatgccaggt gcaaaggact taaactcggc ttgtagtgtt ctaaaagtgc tttccactat
    10561 gcctttagca tcgccacgtc taggtggagc actttccact cgcacattaa aactagaaac
    10621 taaggcttcg acctgatgac tcattaattc gccacggtcc gctagcaaca catctggcaa
    10681 acctacacac ggccagtctg agctactaat ctcaatatca tgctgggcac aaatggccgt
    10741 tttgtcagag caagcattta caaaagcctg catcgccacc acataagacg gattttcaaa
    10801 gccgatataa aagcccgtga tcatccgact aaacacatca atcacaatgt aaagcgttgg
    10861 tcttcctatg attttttggc gatcatgatg atccactaaa taaatatcag caatcgtggc
    10921 atcaatctca taacgactcc cagggcctaa cgcctgagaa gtggctgtac tacttaaggg
    10981 tcgtacgtct tttttatata cccctgcttt aactctagac tttaagcgct gagctttagg
    11041 gtattctcga tcataaaaat aacgaaactg acgtagtgtt gggtaatcct cttggggaat
    11101 gcgaggaaaa tactgagcaa acaagtccac aaatcgtcta taggcaacgg tggtctttgt
    11161 acctttttga tttaacaggt gcttttctat ggtcaaccta aaaaggcgtt caatctcggg
    11221 cgttaccttg gttccttcac ccttaccata ttctctggct cggccaatct ttgctgttcc
    11281 tgtcgctgaa cgtctttccc ctggtgcacc gctgtttttg tagtcaggaa ttaatgcatt
    11341 aggcgtttga ccacgctgcc agtaacggcg taacaactta taaactgtag ccttagtaac
    11401 cttatgttct tggaccacat gctcaacgag ttcgcttctg actttagggt cgaaacgatc
    11461 cttactatta ataataggaa gaatttttcg ataatcctcc tcgcgcttct ggaagctgac
    11521 agaatctaca gacggctctt ctaaatcaag atgtacataa gggtcatcag ttctaactaa
    11581 gcggccttca tctaaatact gcattagcaa caactcagcc ctagcttgtg gaactccttt
    11641 atcagcgctt atttgcatcc agacaacttg gccatcctct atagccaaaa tgcgatacgg
    11701 atcattatca aatagcacaa cctcattaat ttgccacata gcgcaactcc tccatattca
    11761 ctacttggct aatacagaga tctgcacact tatttgccct gaaagactta taaatattga
    11821 acttaataaa accatttgcg gttaaggctc gtatctcact caatgttttg cctaactcca
    11881 aatcataagc aatatcaacc tgcttacaga cattgataat gttttcatct cctttttctt
    11941 gcaggatatg ggccaatggg gatagttgtg ctaaaagctc cgcagaaact tcttctgttt
    12001 tcactgaata aagccattca atattttctt ttactacggg atttatttct ttatcagtaa
    12061 aaatgaacca aggaatttgc ttttgctgcc aatagcgacg ctctagttct agtttttcta
    12121 aggtacgctc gtcttgtaag gctgctgcag gtttgacttg aatagcaaac tgctcaaaag
    12181 gaccatcttt gcagtccact aaaaaatcag tagacataac ctgatctaca ccacgaataa
    12241 caggatgctt aataccacta tctattgcaa tctgcctggt atcactaggt aataagggga
    12301 actgctcgcg tatatctagc acgctgctct cccactcaag actgagaaaa acagcaagct
    12361 ctaagtcaga tagcaaatga tggactcgtc ccgtcttatg agaataaata cggtgggaac
    12421 gacctgaaga aggaacttct tgtactgtta gccatggaat atagtcttta ccatgccctt
    12481 ggccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa gagtttgctt
    12541 tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat ttcagtttac
    12601 aactattctg tctagtttaa gacttgggta ccgagctcga attcgctagc ccaaaaaaac
    12661 gggtatggag aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt
    12721 atggataaaa atgctatggc atagcaaagt gtgacgccgt gcaaataatc aatgtggact
    12781 tttctgccgt gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt
    12841 tgttacagaa tgcttttaat aagcggggtt accggtttgg ttagcgagaa gagccagtaa
    12901 aagacgcagt gacggcaatg tctgatgcaa tatggacaat tggtttcttc tctgaatggc
    12961 gggagtatga aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt
    13021 aatgcccatc tggtggcggg tttaacgccg attgaggcca acggttatct cgattttttt
    13081 atcgaccgac cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg
    13141 gtggtgaaaa atcagggacg agaatttgtt tgccgaccgg gtgatatttt gctgttcccg
    13201 ccaggagaga ttcatcacta cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg
    13261 gtttactttc gtccgcgcgc ctactggcat gaatggctta actggccgtc aatatttgcc
    13321 aatacggggt tctttcgccc ggatgaagcg caccagccgc atttcagcga cctgtttggg
    13381 caaatcatta acgccgggca aggggaaggg cgctattcgg agctgctggc gataaatctg
    13441 cttgagcaat tgttactgcg gcgcatggaa gcgattaacg agtcgctcca tccaccgatg
    13501 gataatcggg tacgcgaggc ttgtcagtac atcagcgatc acctggcaga cagcaatttt
    13561 gatatcgcca gcgtcgcaca gcatgtttgc ttgtcgccgt cgcgtctgtc acatcttttc
    13621 cgccagcagt tagggattag cgtcttaagc tggcgcgagg accaacgtat cagccaggcg
    13681 aagctgcttt tgagcaccac ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt
    13741 gacgatcaac tctatttctc gcgggtattt aaaaaatgca ccggggccag cccgagcgag
    13801 ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc ataattggta
    13861 acgaatcaga caattgacgg cttgacggag tagcataggg tttgcagaat ccctgcttcg
    13921 tccatttgac aggcacatta tgcatcgatg ataagctgtc aaacatgagc agatcctcta
    13981 cgccggacgc atcgtggcct gtgggcggac aataaagtct taaactgaac aaaatagatc
    14041 taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa atcagtccag
    14101 ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc ttcattttct
    14161 gaagtgcaaa ttgcccgtcg tattaaagag gggcgtgggg tcgacgcggc cgtggcgcgc
    14221 ctcctaggtg ctcgagtggc ggccgctatt gacccataaa ctgccaggaa ttggggatcg
    14281 gattcgagct cgcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga
    14341 acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
    14401 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
    14461 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
    14521 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
    14581 caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatac tagtggagga
    14641 agaaaaaa
//
