LOCUS       sequence-226487-        9028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid with CAG promoter driving multicistronic cassette
            H2BmTeal_p2A_HygroR_p2A_CreERt2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, 
            Heller S
  TITLE     Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in
            vitro-generated inner ear sensory epithelia cells and enable otic 
            lineage selection and Cre-recombination.
  JOURNAL   Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013.
  PUBMED    30179592
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013."
COMMENT     Sequence Label: pCAG-mTHC
FEATURES             Location/Qualifiers
     source          1..9028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..99
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             178..555
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             562..1269
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             1279..1335
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1336..2358
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1336..2358
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2371..2427
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2428..3456
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3469..4404
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3517..4410
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(4498..4517)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4563..4618
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4617..4636)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4979..4995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4979..4995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4992..5014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5003..5019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5027..5033,5034..5051,5052..5057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(5105..5560)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5242..5263)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5454..5473
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5587..5691
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5693..6050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5693..5884
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5716..5736)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5901..6036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6085..6879
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6085..6879
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6139..6158)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6749..6768
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7067..7086)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7111..7158
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7487..8075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7976..7995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8249..8628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8630..8828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8783..8803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8846..9026
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
       61 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
      121 gaattcctcg aggatgatcc accggtcgcc ggcaaagaat tcatgaggcc ggccaccatg
      181 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag
      241 gcgcagaaga aagacggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat
      301 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc
      361 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg
      421 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg
      481 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc cgtcaccaag
      541 tacaccagcg ctaaatccac catggtgagc aagggcgagg agaccacaat gggcgtaatc
      601 aagcccgaca tgaagatcaa gctgaagatg gagggcaacg tgaatggcca cgccttcgtg
      661 atcgagggcg agggcgaggg caagccctac gacggcacca acaccatcaa cctggaggtg
      721 aaggagggag cccccctgcc cttctcctac gacattctga ccaccgcgtt cgcctacggc
      781 aacagggcct tcaccaagta ccccgacgac atccccaact acttcaagca gtccttcccc
      841 gagggctact cttgggagcg caccatgacc ttcgaggaca agggcatcgt gaaggtgaag
      901 tccgacatct ccatggagga ggactccttc atctacgaga tacacctcaa gggcgagaac
      961 ttccccccca acggccccgt gatgcagaag aagaccaccg gctgggacgc ctccaccgag
     1021 aggatgtacg tgcgcgacgg cgtgctgaag ggcgacgtca agcacaagct gctgctggag
     1081 ggcggcggcc accaccgcgt tgacttcaag accatctaca gggccaagaa ggcggtgaag
     1141 ctgcccgact atcactttgt ggaccaccgc atcgagatcc tgaaccacga caaggactac
     1201 aacaaggtga ccgtttacga gagcgccgtg gcccgcaact ccaccgacgg catggacgag
     1261 ctgtacaagg gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag
     1321 gagaaccctg gacctatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     1381 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     1441 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     1501 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     1561 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     1621 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     1681 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     1741 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     1801 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     1861 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     1921 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     1981 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     2041 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     2101 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     2161 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     2221 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     2281 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     2341 cgtccgaggg caaaggaacg cggctccggc gcaacaaatt tctctctcct caaacaagca
     2401 ggcgatgttg aagaaaatcc cgggcccatg tccaatttac tgaccgtaca ccaaaatttg
     2461 cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc
     2521 agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg
     2581 tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt
     2641 cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat
     2701 ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat
     2761 gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca
     2821 aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat
     2881 agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta taacaccctg
     2941 ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg
     3001 agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag
     3061 gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc tggtgtagct
     3121 gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct
     3181 gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg
     3241 atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc
     3301 cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa
     3361 gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa
     3421 acaggggcaa tggtgcgcct gctggaagat ggcgatctcg agccatctgc tggagacatg
     3481 agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg
     3541 gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata
     3601 ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg
     3661 accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc
     3721 tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg gctagagatc
     3781 ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct
     3841 aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac
     3901 atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg
     3961 tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg
     4021 aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg
     4081 atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag
     4141 ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac
     4201 agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc
     4261 caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc
     4321 cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg
     4381 gaggcagagg gtttccctgc cacagcttga tagcggccgc actcctcagg tgcaggctgc
     4441 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     4501 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     4561 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     4621 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     4681 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     4741 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     4801 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     4861 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     4921 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     4981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5041 gatgcataaa gtgtaaagcc tggggtgcct aatgagtgaa actcatcaat gtatcttaag
     5101 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     5161 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     5221 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5281 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5341 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5401 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5461 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5521 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     5581 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5641 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     5701 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5761 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5821 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6001 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     6061 gatacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6121 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6181 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6241 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     6301 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6361 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6421 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6481 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6541 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6601 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6661 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6721 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6781 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6841 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6901 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6961 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7021 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     7081 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     7141 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     7201 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     7261 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     7321 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7441 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7501 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7561 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7621 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7681 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7741 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7801 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7861 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7921 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7981 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8041 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8101 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8161 ctgtggataa ccgtattacc gccatgcatt agttattact agcgctaccg gactcagatc
     8221 tcgagctcaa gcttcgaatt ctgcagtcga cattgattat tgactagtta ttaatagtaa
     8281 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     8341 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     8401 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     8461 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     8521 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     8581 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     8641 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     8701 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     8761 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     8821 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     8881 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8941 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9001 ttagtcagtg tggaaaatct ctagcagt
//
