LOCUS       sequence-226488-        9843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression plasmid with CAG promoter driving multicistronic cassette
            H2BmTeal_p2A_HygroR_p2A_CreERt2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Hartman BH, Bscke R, Ellwanger DC, Keymeulen S, Scheibinger M, 
            Heller S
  TITLE     Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate in
            vitro-generated inner ear sensory epithelia cells and enable otic 
            lineage selection and Cre-recombination.
  JOURNAL   Dev Biol. 2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013.
  PUBMED    30179592
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2018 Sep 1. pii: S0012-1606(18)30499-8. doi: 
            10.1016/j.ydbio.2018.08.013."
COMMENT     Sequence Label: pCAG-mTHC
FEATURES             Location/Qualifiers
     source          1..9843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             28..405
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             412..1119
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             1129..1185
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1186..2208
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1186..2208
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2221..2277
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2278..3306
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3319..4254
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3367..4260
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(4348..4367)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4413..4468
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4467..4486)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4829..4845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4829..4845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4842..4864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4853..4869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4877..4883,4884..4901,4902..4907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(4955..5410)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5092..5113)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5304..5323
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5437..5541
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5543..5900
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5543..5734
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5566..5586)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5751..5886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5935..6729
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5935..6729
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5989..6008)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6599..6618
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6917..6936)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6961..7008
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7337..7925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7826..7845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8099..8478
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8481..8756
                     /label=chicken beta-actin promoter
     intron          8757..9765
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     9689..9711
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     9773..9792
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ggcaaagaat tcatgaggcc ggccaccatg ccagagccag cgaagtctgc tcccgccccg
       61 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aagacggcaa gaagcgcaag
      121 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
      181 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
      241 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
      301 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
      361 gtgtccgagg gtactaaggc cgtcaccaag tacaccagcg ctaaatccac catggtgagc
      421 aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg
      481 gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac
      541 gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac
      601 gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta ccccgacgac
      661 atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc
      721 ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc
      781 atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag
      841 aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag
      901 ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag
      961 accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc
     1021 atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg
     1081 gcccgcaact ccaccgacgg catggacgag ctgtacaagg gaagcggagc tactaacttc
     1141 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgaa aaagcctgaa
     1201 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     1261 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     1321 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     1381 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     1441 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     1501 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     1561 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     1621 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     1681 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     1741 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     1801 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     1861 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     1921 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     1981 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2041 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2101 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2161 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaacg cggctccggc
     2221 gcaacaaatt tctctctcct caaacaagca ggcgatgttg aagaaaatcc cgggcccatg
     2281 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
     2341 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
     2401 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
     2461 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     2521 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     2581 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
     2641 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     2701 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     2761 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     2821 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     2881 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     2941 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     3001 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     3061 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
     3121 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
     3181 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     3241 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     3301 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
     3361 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
     3421 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
     3481 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
     3541 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
     3601 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
     3661 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
     3721 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
     3781 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
     3841 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
     3901 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
     3961 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
     4021 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
     4081 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
     4141 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
     4201 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
     4261 tagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     4321 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     4381 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     4441 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     4501 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     4561 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     4621 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     4681 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     4741 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     4801 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4861 cgctcacaat tccacacaac atacgagccg gatgcataaa gtgtaaagcc tggggtgcct
     4921 aatgagtgaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     4981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     5041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     5101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     5161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     5221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     5281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     5341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     5401 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5461 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5521 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     5581 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5641 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5701 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5881 gaggcctagg cttttgcaaa gatcgatcaa gatacaggat gaggatcgtt tcgcatgatt
     5941 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6001 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6061 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     6121 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6181 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6241 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6301 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6361 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6421 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     6481 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6541 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6601 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6661 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6721 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6781 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6841 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6901 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     6961 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     7021 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     7081 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     7141 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     7201 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7261 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7321 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7381 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7441 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7501 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7561 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7621 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7681 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7741 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7801 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7861 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7921 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7981 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
     8041 agttattact agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     8101 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     8161 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     8221 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     8281 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     8341 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     8401 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     8461 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     8521 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     8581 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
     8641 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     8701 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     8761 tcgctgcgtt gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc
     8821 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     8881 gtaattagcg cttggtttaa tgacggctcg tttcttttct gtggctgcgt gaaagcctta
     8941 aagggctccg ggagggccct ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt
     9001 gtgtgcgtgg ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc
     9061 gcggcgcggg gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc
     9121 cccgcggtgc gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     9181 gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc acccccctcc
     9241 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg
     9301 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
     9361 tcgggccggg gagggctcgg gggaggggcg cggcggcccc ggagcgccgg cggctgtcga
     9421 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     9481 cctttgtccc aaatctggcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     9541 ggcgcgggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     9601 gtcgccgcgc cgccgtcccc ttctccatct ccagcctcgg ggctgccgca gggggacggc
     9661 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     9721 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     9781 ctggttattg tgctgtctca tcattttggc aaagaattcc tcgaggatga tccaccggtc
     9841 gcc
//
