LOCUS       sequence-226496-       10020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI,
            Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA, 
            Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, 
            Goessling W, Hahn WC
  TITLE     beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex 
            for Survival and Tumorigenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026. 
            Epub 2012 Dec 13.
  PUBMED    23245941
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.026"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1457-73"
COMMENT     Sequence Label: CTNNB1 (mut) in pLX304
FEATURES             Location/Qualifiers
     source          1..10020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     36..56
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        38..56
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        84..310
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             311..491
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    538..663
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1156..1389
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1574..1618
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1793..2293
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2212..2230
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2305..2700
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2760..2877
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2994..3297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3298..3501
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3452..3472
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3524..3548
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3593..3628
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     protein_bind    complement(5847..5871)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5873..5914
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5956..6544
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6009..6029)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6427..6438)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6616..6849
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6927..7048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6964..6983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7018..7037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7088..7223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7243..7262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7244..7262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7272..7289)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7272..7288)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7281..7303)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7430..7885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7517..7536)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7911..8015
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8016..8084,8085..8876)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8234..8253)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9047..9635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9536..9555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9789..9806
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9923..9944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9959..9964,9965..9982,9983..9989)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9997..10013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10002..10020,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
       61 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      121 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      181 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      241 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
      301 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      541 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      601 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      661 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
      721 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      781 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
      841 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
      901 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
      961 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1021 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1081 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1141 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1201 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1261 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1321 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1381 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1441 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1501 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1561 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1621 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     1681 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     1741 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct
     1801 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     1861 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     1921 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     1981 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     2041 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     2101 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     2161 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     2221 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     2281 cctctctccc cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc
     2341 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     2401 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     2461 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     2521 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     2581 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     2641 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     2701 ctgcagctgc agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat
     2761 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2821 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2881 gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat
     2941 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3001 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3061 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3301 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3361 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3421 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     3481 gaggtctata taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa
     3541 agttggcatg gctactcaag ctgatttgat ggagttggac atggccatgg agccggacag
     3601 aaaagctgct gtcagccact ggcagcagca gtcttacttg gatgctggaa tccatgctgg
     3661 tgccaccgcc acagctcctg ccctgagtgg caagggcaac cctgaggaag aagatgttga
     3721 cacctcccaa gtcctttatg aatgggagca aggcttttcc cagtccttca cgcaagagca
     3781 agtagctgat attgacgggc agtatgcaat gactagggct cagagggtcc gagctgccat
     3841 gttccctgag acgctagatg agggcatgca gatcccatcc acgcagtttg acgctgctca
     3901 tcccactaat gtccagcgct tggctgaacc atcacagatg ttgaaacatg cagttgtcaa
     3961 tttgattaac tatcaggatg acgcggaact tgccacacgt gcaattcctg agctgacaaa
     4021 actgctaaac gatgaggacc aggtggtagt taataaagct gctgttatgg tccatcagct
     4081 ttccaaaaag gaagcttcca gacatgccat catgcgctcc cctcagatgg tgtctgccat
     4141 tgtacgcacc atgcagaata caaatgatgt agagacagct cgttgtactg ctgggactct
     4201 gcacaacctt tctcaccacc gcgagggctt gctggccatc tttaagtctg gtggcatccc
     4261 agcgctggtg aaaatgcttg ggtcaccagt ggattctgta ctgttctacg ccatcacgac
     4321 actgcataat ctcctgctcc atcaggaagg agctaaaatg gcagtgcgcc tagctggtgg
     4381 actgcagaaa atggttgctt tgctcaacaa aacaaacgtg aaattcttgg ctattacaac
     4441 agactgcctt cagatcttag cttatggcaa tcaagagagc aagctcatca ttctggccag
     4501 tggtggaccc caagccttag taaacataat gaggacctac acttatgaga agcttctgtg
     4561 gaccacaagc agagtgctga aagtgctgtc tgtctgctct agcaacaagc cggccattgt
     4621 agaagctggt gggatgcagg cactggggct tcatctgaca gacccaagtc agcgacttgt
     4681 tcaaaactgt ctttggactc tcagaaacct ttcagatgca gcgactaagc aggaagggat
     4741 ggaaggcctc cttgggactc tagtgcagct tctgggttcc gatgatataa atgtggtcac
     4801 ctgtgcagct ggaattctct ctaacctcac ttgcaataat tacaaaaaca agatgatggt
     4861 gtgccaagtg ggtggcatag aggctcttgt acgcaccgtc cttcgtgctg gtgacaggga
     4921 agacatcact gagcctgcca tctgtgctct tcgtcatctg accagccggc atcaggaagc
     4981 cgagatggcc cagaatgccg ttcgccttca ttatggactg cctgttgtgg ttaaactcct
     5041 gcacccacca tcccactggc ctctgataaa ggcaactgtt ggattgattc gaaaccttgc
     5101 cctttgccca gcaaatcatg cgcctttgcg ggaacagggt gctattccac gactagttca
     5161 gctgcttgta cgagcacatc aggacaccca acggcgcacc tccatgggtg gaacgcagca
     5221 gcagtttgtg gagggcgtgc gcatggagga gatagtagaa gggtgtactg gagctctcca
     5281 catccttgct cgggacgttc acaaccggat tgtaatccga ggactcaata ccattccatt
     5341 gtttgtgcag ttgctttatt ctcccattga aaatatccaa agagtagctg caggggtcct
     5401 ctgtgaactt gctcaggaca aggaggctgc agaggccatt gaagctgagg gagccacagc
     5461 tcccctgaca gagttactcc actccaggaa tgaaggcgtg gcaacatacg cagctgctgt
     5521 cctattccga atgtctgagg acaagccaca ggattacaag aagcggcttt cagtcgagct
     5581 gaccagttcc ctcttcagga cagagccaat ggcttggaat gagactgcag atcttggact
     5641 ggacattggt gcccagggag aagcccttgg atatcgccag gatgatccca gctaccgttc
     5701 ttttcactct ggtggatacg gccaggatgc cttggggatg gaccctatga tggagcatga
     5761 gatgggtggc caccaccctg gtgctgacta tccagttgat gggctgcctg atctgggaca
     5821 cgcccaggac ctcatggatg ggctgccccc aactttcttg tacaaagtgg ttggtaagcc
     5881 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg
     5941 gcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6001 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6061 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6121 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6181 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6241 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6301 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6361 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6421 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6481 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6541 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6601 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6661 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6721 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6781 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6841 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6901 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6961 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7021 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7081 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7141 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7201 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7261 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7321 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7381 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7441 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7501 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7561 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7621 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7681 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaataatgg actcttgttc
     7741 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7801 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7861 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7921 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7981 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8041 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8101 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8161 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8221 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8281 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8341 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8401 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8461 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8521 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8581 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8641 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8701 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8761 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8821 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8881 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8941 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9001 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9061 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9121 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9181 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9241 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9301 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9361 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9421 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9481 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9541 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9601 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9661 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9721 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9781 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9841 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9901 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9961 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
//
