LOCUS       sequence-226500-       15508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15508)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-153
FEATURES             Location/Qualifiers
     source          1..15508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      738..832
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             876..1535
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(942..961)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1600..1686
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2115..2993)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3020..3304
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3227..3246
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(9995..10012)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      10165..10251
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10343..10370
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        10389..10480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10481..10549,10550..11341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10699..10718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(11470..11489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            11512..11621
                     /note="incP origin of transfer"
     primer_bind     complement(11659..11678)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      12361..12583
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             12631..13581
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  14014..14179
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             14353..15147
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     GQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDVGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 gtaattagct gagcttggac tcctgttgat agatccagta atgacctcag aactccatct
      781 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta
      841 gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
      901 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
      961 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     1021 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     1081 ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     1141 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     1201 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     1261 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     1321 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     1381 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     1441 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     1501 aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
     1561 cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag
     1621 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1681 acaaatccgc cgctaggagc ttgcggccgc caggggtcaa tagcggccgc cactcgagca
     1741 cctaggaggc gcgccacggc cgcgtcgacc ccacgcccct ctttaatacg acgggcaatt
     1801 tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca
     1861 cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc
     1921 atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacag gccacgatgc
     1981 gtccggcgta gaggatctgc tcatgtttga cagcttatca tcgatgcata atgtgcctgt
     2041 caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc
     2101 tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg
     2161 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
     2221 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
     2281 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
     2341 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
     2401 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc
     2461 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
     2521 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
     2581 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac
     2641 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
     2701 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc
     2761 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt
     2821 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
     2881 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
     2941 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc
     3001 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac
     3061 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt
     3121 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac
     3181 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt
     3241 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     3301 ccatacccgt ttttttgggc tagcgaattc gagctcggta cccaagtctt aaactagaca
     3361 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     3421 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     3481 gcgtggccaa gggcatggta aagactatat tccatggcta acagtacaag aagttccttc
     3541 ttcaggtcgt tcccaccgta tttattctca taagacggga cgagtccatc atttgctatc
     3601 tgacttagag cttgctgttt ttctcagtct tgagtgggag agcagcgtgc tagatatacg
     3661 cgagcagttc cccttattac ctagtgatac caggcagatt gcaatagata gtggtattaa
     3721 gcatcctgtt attcgtggtg tagatcaggt tatgtctact gattttttag tggactgcaa
     3781 agatggtcct tttgagcagt ttgctattca agtcaaacct gcagcagcct tacaagacga
     3841 gcgtacctta gaaaaactag aactagagcg tcgctattgg cagcaaaagc aaattccttg
     3901 gttcattttt actgataaag aaataaatcc cgtagtaaaa gaaaatattg aatggcttta
     3961 ttcagtgaaa acagaagaag tttctgcgga gcttttagca caactatccc cattggccca
     4021 tatcctgcaa gaaaaaggag atgaaaacat tatcaatgtc tgtaagcagg ttgatattgc
     4081 ttatgatttg gagttaggca aaacattgag tgagatacga gccttaaccg caaatggttt
     4141 tattaagttc aatatttata agtctttcag ggcaaataag tgtgcagatc tctgtattag
     4201 ccaagtagtg aatatggagg agttgcgcta tgtggcaaat taatgaggtt gtgctatttg
     4261 ataatgatcc gtatcgcatt ttggctatag aggatggcca agttgtctgg atgcaaataa
     4321 gcgctgataa aggagttcca caagctaggg ctgagttgtt gctaatgcag tatttagatg
     4381 aaggccgctt agttagaact gatgaccctt atgtacatct tgatttagaa gagccgtctg
     4441 tagattctgt cagcttccag aagcgcgagg aggattatcg aaaaattctt cctattatta
     4501 atagtaagga tcgtttcgac cctaaagtca gaagcgaact cgttgagcat gtggtccaag
     4561 aacataaggt tactaaggct acagtttata agttgttacg ccgttactgg cagcgtggtc
     4621 aaacgcctaa tgcattaatt cctgactaca aaaacagcgg tgcaccaggg gaaagacgtt
     4681 cagcgacagg aacagcaaag attggccgag ccagagaata tggtaagggt gaaggaacca
     4741 aggtaacgcc cgagattgaa cgccttttta ggttgaccat agaaaagcac ctgttaaatc
     4801 aaaaaggtac aaagaccacc gttgcctata gacgatttgt ggacttgttt gctcagtatt
     4861 ttcctcgcat tccccaagag gattacccaa cactacgtca gtttcgttat ttttatgatc
     4921 gagaataccc taaagctcag cgcttaaagt ctagagttaa agcaggggta tataaaaaag
     4981 acgtacgacc cttaagtagt acagccactt ctcaggcgtt aggccctggg agtcgttatg
     5041 agattgatgc cacgattgct gatatttatt tagtggatca tcatgatcgc caaaaaatca
     5101 taggaagacc aacgctttac attgtgattg atgtgtttag tcggatgatc acgggctttt
     5161 atatcggctt tgaaaatccg tcttatgtgg tggcgatgca ggcttttgta aatgcttgct
     5221 ctgacaaaac ggccatttgt gcccagcatg atattgagat tagtagctca gactggccgt
     5281 gtgtaggttt gccagatgtg ttgctagcgg accgtggcga attaatgagt catcaggtcg
     5341 aagccttagt ttctagtttt aatgtgcgag tggaaagtgc tccacctaga cgtggcgatg
     5401 ctaaaggcat agtggaaagc acttttagaa cactacaagc cgagtttaag tcctttgcac
     5461 ctggcattgt agagggcagt cggatcaaaa gccatggtga aacagactat aggttagatg
     5521 catctctgtc ggtatttgag ttcacacaaa ttattttgcg tacgatctta ttcagaaata
     5581 accatctggt gatggataaa tacgatcgag atgctgattt tcctacagat ttaccgtcta
     5641 ttcctgtcca gctatggcaa tggggtatgc agcatcgtac aggtagttta agggctgtgg
     5701 agcaagagca gttgcgagta gcgttactgc ctcgccgaaa ggtctctatt tcttcatttg
     5761 gcgttaattt gtggggtttg tattactcgg ggtcagagat tctgcgtgag ggttggttgc
     5821 agcggagcac tgatatagct agacctcaac atttagaagc ggcttatgac ccagtgctgg
     5881 ttgatacgat ttatttgttt ccgcaagttg gcagccgtgt attttggcgc tgtaatctga
     5941 cggaacgtag tcggcagttt aaaggtctct cattttggga ggtttgggat atacaagcac
     6001 aagaaaaaca caataaagcc aatgcgaagc aggatgagtt aactaaacgc agggagcttg
     6061 aggcgtttat tcagcaaacc attcagaaag cgaataagtt aacgcccagt actactgagc
     6121 ccaaatcaac acgcattaag cagattaaaa ctaataaaaa agaagccgtg acctcggagc
     6181 gtaaaaaacg tgcggagcat ttgaagccaa gctcttcagg tgatgaggct aaagttattc
     6241 ctttcaacgc agtggaagcg gatgatcaag aagattacag cctacccaca tacgtgcctg
     6301 aattatttca ggatccacca gaaaaggatg agtcatgagt gctacccgga ttcaagcagt
     6361 ttatcgtgat acgggggtag aggcttatcg tgataatcct tttatcgagg ccttaccacc
     6421 attacaagag tcagtgaata gtgctgcatc actgaaatcc tctttacagc ttacttcctc
     6481 tgacttgcaa aagtcccgtg ttatcagagc tcataccatt tgtcgtattc cagatgacta
     6541 ttttcagcca ttaggtacgc atttgctact aagtgagcgt atttcggtca tgattcgagg
     6601 tggctacgta ggcagaaatc ctaaaacagg agatttacaa aagcatttac aaaatggtta
     6661 tgagcgtgtt caaacgggag agttggagac atttcgcttt gaggaggcac gatctacggc
     6721 acaaagctta ttgttaattg gttgttctgg tagtgggaag acgacctctc ttcatcgtat
     6781 tctagccacg tatcctcagg tgatttacca tcgtgaactc aatgtagagc aggtggtgta
     6841 tttgaaaata gactgctcgc ataatggttc gctaaaagaa atctgcttga attttttcag
     6901 agcgttggat cgagccttgg gctcgaacta tgagcgtcgt tatggcttaa aacgtcatgg
     6961 tatagaaacc atgttggctt tgatgtcgca aatagccaat gcacatgctt tagggttgtt
     7021 ggttattgat gaaattcagc atttaagccg ctctcgttcg ggtggatctc aagagatgct
     7081 gaactttttt gtgacgatgg tgaatattat tggcgtacca gtgatgttga ttggtacccc
     7141 taaagcacga gagatttttg aggctgattt gcggtctgca cgtagagggg cagggtttgg
     7201 agctatattc tgggatccta tacaacaaac gcaacgtgga aagcccaatc aagagtggat
     7261 cgcttttacg gataatcttt ggcaattaca gcttttacaa cgcaaagatg cgctgttatc
     7321 ggatgaggtc cgtgatgtgt ggtatgagct aagccaagga gtgatggaca ttgtagtaaa
     7381 actttttgta ctcgctcagc tccgtgcgct agctttaggc aatgagcgta ttaccgctgg
     7441 tttattgcgg caagtgtatc aagatgagtt aaagcctgtg caccccatgc tagaggcatt
     7501 acgctcgggt atcccagaac gcattgctcg ttattctgat ctagtcgttc ccgagattga
     7561 taaacggtta atccaacttc agctagatat cgcagcgata caagaacaaa caccagaaga
     7621 aaaagccctt caagagttag ataccgaaga tcagcgtcat ttatatctga tgctgaaaga
     7681 ggattacgat tcaagcctgt taattcccac tattaaaaaa gcgtttagcc agaatccaac
     7741 gatgacaaga caaaagttac tgcctcttgt tttgcagtgg ttgatggaag gcgaaacggt
     7801 agtgtcagaa ctagaaaagc cctccaagag taaaaaggtt tcggctataa aggtagtcaa
     7861 gcccagcgac tgggatagct tgcctgatac ggatttacgt tatatctatt cacaacgcca
     7921 acctgaaaaa accatgcatg aacggttaaa agggaaaggg gtaatagtgg atatggcgag
     7981 cttatttaaa caagcaggtt agccatgaga aactttcctg ttccgtactc gaatgagctg
     8041 atttatagca ctattgcacg ggcaggcgtt tatcaaggga ttgttagtcc taagcagctg
     8101 ttggatgagg tgtatggcaa ccgcaaggtg gtcgctacct taggtctgcc ctcgcattta
     8161 ggtgtgatag caagacatct acatcaaaca ggacgttacg ctgttcagca gcttatttat
     8221 gagcatacct tattcccttt atatgctccg tttgtaggca aggagcgccg agacgaagct
     8281 attcggttaa tggagtacca agcgcaaggt gcggtgcatt taatgctagg agtcgctgct
     8341 tctagagtta agagcgataa ccgctttaga tactgccctg attgcgttgc tcttcagcta
     8401 aataggtatg gggaagcctt ttggcaacga gattggtatt tgcccgcttt gccatattgt
     8461 ccaaaacacg gtgctttagt cttctttgat agagctgtag atgatcaccg acatcaattt
     8521 tgggctttgg gtcatactga gctgctttca gactacccca aagactccct atctcaatta
     8581 acagcactag ctgcttatat agcccctctg ttagatgctc cacgagcgca agagctttcc
     8641 ccaagccttg agcagtggac gctgttttat cagcgcttag cgcaggatct agggctaacc
     8701 aaaagcaagc acattcgtca tgacttggtg gcggagagag tgaggcagac ttttagtgat
     8761 gaggcactag agaaactgga tttaaagttg gcagagaaca aggacacgtg ttggctgaaa
     8821 agtatattcc gtaagcatag aaaagccttt agttatttac agcatagtat tgtgtggcaa
     8881 gccttattgc caaaactaac ggttatagaa gcgctacagc aggcaagtgc tcttactgag
     8941 cactctataa cgacaagacc tgttagccag tctgtgcaac ctaactctga agatttatct
     9001 gttaagcata aagactggca gcaactagtg cataaatacc aaggaattaa ggcggcaaga
     9061 cagtctttag agggtggggt gctatacgct tggctttacc gacatgacag ggattggcta
     9121 gttcactgga atcaacagca tcaacaagag cgtctggcac ccgcccctag agttgattgg
     9181 aaccaaagag atcgaattgc tgtacgacaa ctattaagaa tcataaagcg tctagatagt
     9241 agccttgatc acccaagagc gacatcgagc tggctgttaa agcaaactcc taacggaacc
     9301 tctcttgcaa aaaatctaca gaaactgcct ttggtagcgc tttgcttaaa gcgttactca
     9361 gagagtgtgg aagattatca aattagacgg attagccaag cttttattaa gcttaaacag
     9421 gaagatgttg agcttaggcg ctggcgatta ttaagaagtg caacgttatc taaagagcgg
     9481 ataactgagg aagcacaaag attcttggaa atggtttatg gggaagagtg agtggttagg
     9541 ctagctacat ttaatgacaa tgtgcaggtt gtacatattg gtcatttatt ccgtaactcg
     9601 ggtcataagg agtggcgtat ttttgtttgg tttaatccaa tgcaagaacg gaaatggact
     9661 cgatttactc atttgccttt attaagtcga gctaaggtgg ttaacagtac aacaaagcaa
     9721 ataaataagg cggatcgtgt gattgagttt gaagcatcgg atcttcaacg agccaaaata
     9781 atcgattttc ctaatctctc gtcctttgct tccgtacgca acaaggatgg agcgcagagt
     9841 tcatttattt acgaagctga aacaccatat agcaagactc gttatcacat cccacagtta
     9901 gagctagctc ggtcattatt tttaggggat cctctagagt cgacctgcag gcatgcaagc
     9961 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
    10021 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
    10081 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
    10141 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
    10201 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
    10261 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
    10321 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
    10381 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10441 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10501 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10561 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    10621 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    10681 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
    10741 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10801 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10861 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    10921 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    10981 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
    11041 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11101 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11161 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11221 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11281 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11341 actgtcagac caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg
    11401 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    11461 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc gccggccagc
    11521 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
    11581 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggcggcatc accggcgcca
    11641 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
    11701 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
    11761 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
    11821 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgatccc
    11881 cgacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg cattagccag
    11941 tctgaatgac ctgtcacggg ataatccgaa gtggtcagac tggaaaatca gagggcagga
    12001 actgctgaac agcaaaaagt cagatagcac cacatagcag acccgccata aaacgccctg
    12061 agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga atccataaaa
    12121 ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca gcgaactgaa
    12181 tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg aatattcagc
    12241 gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt
    12301 agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt
    12361 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
    12421 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
    12481 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
    12541 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
    12601 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
    12661 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
    12721 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
    12781 acttataacc aatacgttca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
    12841 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
    12901 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
    12961 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
    13021 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
    13081 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
    13141 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
    13201 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
    13261 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
    13321 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
    13381 acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca
    13441 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat
    13501 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
    13561 gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag
    13621 actaacaaac aaaagtagaa caactgttca ccgttacata tcaaagggaa aactgtccat
    13681 atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac gagtttttgg
    13741 tgcatttaaa gctgttcacc atgaacagat cgacaatgta acagatgaac agcatgtaac
    13801 acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa ttgaacacct
    13861 gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg cgatgctgct
    13921 tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg ggaaaaaatc
    13981 atggcaattc tggaagaaat agcgctttca gcctgtgggc ggacaaaata gttgggaact
    14041 gggaggggtg gaaatggagt ttttaaggat tatttaggga agagtgacaa aatagatggg
    14101 aactgggtgt agcgtcgtaa gctaatacga aaattaaaaa tgacaaaata gtttggaact
    14161 agatttcact tatctggttg gtcgacacta gtattaccct gttatcccta gatttaaatg
    14221 atatcggatc ctagtaagcc acgttttaat taatcagatc ccataaactg ccaggaattg
    14281 gggatcggat tcgagctaag aattcccttg gggtatcttt aaatactgta gaaaagagga
    14341 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
    14401 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
    14461 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
    14521 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
    14581 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
    14641 ctttgctcgg aagagtatga agatggacaa agccctgaaa agattatcga gctgtatgcg
    14701 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
    14761 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
    14821 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
    14881 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
    14941 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
    15001 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatgtcgg ggaagaacag
    15061 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
    15121 tatattttac tggatgaatt gttttagagc tcgcacgctg ccgcaagcac tcagggcgca
    15181 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
    15241 ggatgaatgt cagctactgg gctatctgga caakggaaaa cgcaagcgca aagagaaagc
    15301 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
    15361 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
    15421 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag
    15481 agacaggata ctagtggagg aagaaaaa
//
