LOCUS       sequence-226501-       20460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic expression of luc, ekivR, esyn1 using P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20460)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 20460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS32.372
FEATURES             Location/Qualifiers
     source          1..20460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          341..456
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             2223..2279
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3660..3716
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(15795..15814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15796..15814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15821..15838)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15821..15837)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15830..15852)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15979..16407
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16066..16085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16276..16297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        16460..16564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16565..16633,16634..17425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16783..16802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17596..18184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18085..18104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18338..18355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    18472..18493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18508..18513,18514..18531,18532..18538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18546..18562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18551..18573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18570..18586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18570..18586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        18607..18625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     18653..18669
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     18654..18670
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          19234..19349
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(19367..19984,19985..19990,19991..20026,20027..20029,
                     20030..20065,20066..20068,20069..20104,20105..20113)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcc ccatcttcag
      301 tatattcatc ttcccatcca agaaccttta tttcccctaa gtaagtactt tgctacatcc
      361 atactccatc cttcccatcc cttattcctt tgaacctttc agttcgagct ttcccacttc
      421 atcgcagctt gactaacagc taccccgctt gagcagacat caccgtttaa acaccatgga
      481 ggacgccaag aacatcaaga agggccccgc ccccttctac cccctcgagg acggcaccgc
      541 cggcgagcag ctccacaagg ccatgaagcg ctacgccctc gtccccggca ccatcgcctt
      601 cgtaggtttc ctccagctct cgcctccagc acccgaggca cacctcgggc atcttcacaa
      661 caacagacac tgacatctca ttctcacaga ccgacgccca catcgaggtc aacatcacct
      721 acgccgagta cttcgagatg tccgtccgcc tcgccgaggc catgaagcgc tacggcctca
      781 acaccaacca ccgcatcgtc gtctgctccg agaactccct ccagttcttc atgcccgtcc
      841 tcggcgccct cttcatcggc gtcgccgtcg cccccgccaa cgacatctac aacgagcgcg
      901 agctcctcaa ctccatgaac atctcccagc ccaccgtcgt cttcgtctcc aagaagggcc
      961 tccagaagat cctcaacgtc cagaagaagc tccccatcat ccagaagatc atcatcatgg
     1021 actccaagac cgactaccag ggcttccagt ccatgtacac cttcgtcacc tcccacctcc
     1081 cccccggctt cagcgagtac gacttcgtcc ccgagtcctt cgaccgcgac aagaccatcg
     1141 ccctcatcat gaactcctcc ggctccaccg gcctccccaa gggcgtcgcc ctcccccacc
     1201 gcaccgcctg cgtccgcttc tcccacgccc gcgaccccat cttcggcaac cagatcatcc
     1261 ccgacaccgc catcctctcc gtcgtcccct tccaccacgg cttcggcatg ttcaccaccc
     1321 tcggctacct catctgcggc ttccgcgtcg tcctcatgta ccgcttcgag gaggagctct
     1381 tcctccgctc cctccaggac tacaagatcc agtccgccct cctcgtcccc accctcttct
     1441 ccttcttcgc caagtccacc ctcatcgaca agtacgacct ctccaacctc cacgagatcg
     1501 cctccggcgg cgcccccctc tccaaggagg tcggcgaggc cgtcgccaag cgcttccacc
     1561 tccccggcat ccgccagggc tacggcctca ccgagaccac ctccgccatc ctcatcaccc
     1621 ccgagggcga cgacaagccc ggcgccgtcg gcaaggtcgt ccccttcttc gaggccaagg
     1681 tcgtcgacct cgacaccggc aagaccctcg gcgtcaacca gcgcggcgag ctctgcgtcc
     1741 gcggccccat gatcatgtcc ggctacgtca acaaccccga ggccaccaac gccctcatcg
     1801 acaaggacgg ctggctccac tccggcgaca tcgcctactg ggacgaggac gagcacttct
     1861 tcatcgtcga ccgcctcaag tccctcatca agtacaaggg ctaccaggtc gcccccgccg
     1921 agctcgagtc catcctcctc cagcacccca acatcttcga cgccggcgtc gccggcctcc
     1981 ccgacgacga cgccggcgag ctccccgccg ccgtcgtcgt cctcgagcac ggcaagacca
     2041 tgaccgagaa ggagatcgtc gactacgtcg cctcccaggt caccaccgcc aagaagctcc
     2101 gcggcggcgt cgtcttcgtc gacgaggtcc ccaagggcct caccggcaag ctcgacgccc
     2161 gcaagatccg cgagatcctc atcaaggcca agaagggcgg caagtccaag ctcggctccg
     2221 gcgccaccaa cttctccctc ctgaagcagg ccggtgacgt cgaggagaac cccggcccca
     2281 tggaaaagcc tgtggcctca caagtccacc ccagctggct aacagctctt ctacctgaat
     2341 ccagaatacc ccccaagctg tttgcctggt cctcagccaa catccgccca gcaagctcct
     2401 ccaagctcgg aggctctgcc accaacatcc accatgcaga gtgttcacat ccaaccccaa
     2461 actccacagc aagctctgag cagcgcatct tcatcgttgg tcccggcaac ataggtaggc
     2521 tctacgcaag ccacatggtc cgccgcccaa atcccctccc catcactctc gtcgtcccca
     2581 gaaaggaact cctatcgcaa tgggcaggct gtgaaggcgt gggccttata gacccgggtc
     2641 gcggcaaggt cctcaaaagc aagcgcttca ccgtcgagtg gtggacggag actagaccac
     2701 agtatggtcc tgtgagagag gtggcagacg gaaagaagct gcgcaacgtg ttcatatcca
     2761 caaaggcatc cgctgggctg gccgaggcag atcgtcttcg tcggtatttg ggcaagtgta
     2821 gttctgtcgt ctttgcgcag aacggagtat gcaagctgtg gcctcctcat gggccgttat
     2881 acgtcgccgg tcgataccct cctggtgatg ctcctacctt ctcatcctgc gtcgtgaatc
     2941 atggcgttgc atcagcgggg ccttttctga gcattcacgc agctccggca gatgcatcca
     3001 tcggacctgt cctctggcct tcccatccga tgccgcacaa gaaacccctg aacgacttct
     3061 tcacaagata cattgctact acacccttcc taaacacaaa gcaagtttcc tctggtgagc
     3121 tctggcttat tcagctggag aaacttgtta ccaatgccgc catcaacccc ctcacagctt
     3181 tgctacgctg caaaacggga cagctgttca caacccatga ccccacagac cctcttacgc
     3241 gcgtgctgga caggttgcta tggcaggcca gcgccgtgat tcagggtctt atcaaccacg
     3301 acgcgagtgt cgacatcgtc gcttcctacg ccgagcacct ccaccagacc gaaccaagca
     3361 gcgactcaag ccttgtcgca gtacggacca agttaacaga gcggttctcc cagcctacct
     3421 tgagagcgaa gctattcgcc tttggtcgca agatatacga gcatcgcagc tcgatgcttc
     3481 aggatgttga ggccggcagg aaaacggaga taagagattt caacgggtgg gttgtggata
     3541 tggcgagttt tctggggacg gggctgaatg tgagcgtgca tcagagtttg attgtgctga
     3601 ttgaacgcgg tgaggttttg aacaaggatg agttggctag gaggacatta ggctccggcg
     3661 ccaccaactt ctccctcctg aagcaggccg gtgacgtcga ggagaacccc ggccccatgt
     3721 cactccacac cccaagtgac ggtcaacaag acccagcgtt agcctccaaa accctctgtg
     3781 agcaaatctc gcgagccctc ggcctcggcc aggacaagat tgagaatatc ttcccaggga
     3841 cacccttcca gcgggacgtc atagactgcg ccgcagacga caagcagcgc gccgtgggcc
     3901 acgctgtctt tgaaattccc aaagatatag acgcggcccg cctggcggct gcgtggaagg
     3961 agaccgtgct tcacacccct gctctgcgga cctgcacgtt tacgtccaaa tctggggatg
     4021 tgcttcaggt ggtcctgaga gatagctttg tcttttcgtg gatgtctggg ccgtctgtgg
     4081 atcttaagga ggcggttgtg caagacgagg ctgctgctgc tttggctggc ccgcgctgta
     4141 accgcttcgt ccttcttgag gaccctgata caaaggaacg tcagctcatc tggacgttca
     4201 gccatgctct cgttgatagt accttccagg aacgtatcct tcgacgagtc ctcaaagctt
     4261 acaaggatgc caatgacgaa catcctcgcc agttcgagac accagattct tcccaggcca
     4321 cgcccgagga ggatttacag cccaatccat ccaagatgct caagatccct caagccgcag
     4381 atatggaccg tgctgttgag ttttggaagg atcatctaag tggcctgaat gcttctgcct
     4441 tcccgcattt gtcctctcat ctgtctatgc cacatccaga tgccaaggca gagcatagaa
     4501 tctcctactc gtcttcagca cagcaaaaaa tgtccagtgc tactatttgc cgaactgccc
     4561 tggcaattct gttatctcgt tatacgcact ctcctgaagc actctttggc attgtaactg
     4621 agcaaactcc tctactggag gagcaactca tgcttgatgg cccaacacgt acagtcgtac
     4681 caatccgcgt atcctgtgct tctgaacagt cagtgtcaga tatcatgagc acgattgact
     4741 cctacgatca gaccatgcgg cagtttgccc acgctggtct tcgcaacatc gccagtgctg
     4801 gagacgatga atctgccgcc tgcgggttcc agaccgttct cttggtctca gatggagacg
     4861 cccagccagc atctacttgg gaaattctca agaaaactga agagcctgag ggtttcatcc
     4921 cctgtacgaa ccgtgctctg cttctaagtt gccagatgac cagctctgga gcacacctaa
     4981 ccgccagata cgaccagagt atcatcgacg ctgagcaaat ggcccgactc ctgcgacaac
     5041 taggccatct gatccaaaat cttcaaacct ccacagatct acccgtggaa aaggtggaca
     5101 tgatgacgca agaagactgg ttagagattg agaggtggaa ctcagactcc atcgacgcac
     5161 aagacacgct tatccacagc gagatgttga agtggacttc tcagtcgcct aacaaagctg
     5221 ccgtcgccgc gtgggatgga gagtggacgt atgctgagct ggacaatgta tcttcgcgcc
     5281 tggctcagca tatcaactct attgacctgg gcaaggagca cgcgatagta cccatctact
     5341 ttgagaaatc gaaatgggtc gttgcttcga tgctggctgt gctcaaggct ggccatgcgt
     5401 ttactctcat tgaccccagt gatccaccag ctcgaacagc acaggtcgtg cagcaaacct
     5461 ctgcgacagt tgctcttact tccaagctcc atcgcgagac tgttcaatca actgtcgggc
     5521 gctgcattgt tgtcgacgaa gagtttgtca aatccctccc ccagtccagc gaactctcag
     5581 cgtctgtaaa ggcacatgac ctggcatatg tcatcttcac ctcgggcagc acaggcattc
     5641 caaagggaat catgattgag caccgctcat tttcatcctg cgccatcaaa ttcggtcccg
     5701 cgcttggaat aacctccgac acacgcgctc ttcagtttgg atctcatgcc tttggcgcgt
     5761 gtatcctcga gatcatgacg acgctcatcc acggaggctg tgtctgtatc ccctctgacg
     5821 atgaccgcat gaacaacgtc cttgagttca tcaaccggac taacgtcaac tgggtcatgg
     5881 caacgccttc gtacatgggg acattccagc cagaggttgt tcctggtttg aagaccttgg
     5941 tattggtagg tgagcagatg tctgcttctg tgaatgaggt ctgggctcct cgtgttcaac
     6001 tcctgaacgg ttacggacag agcgagagtt cttctatctg ctgcgtagcc aagatcagcc
     6061 ctggttcatc tgagccaaac aatattggcc acgcagtagg cgcacattct tggatcgttg
     6121 accctgagga tcccaatcgc ttggctccca tcggtgccgt tggcgagctg gtcatcgaga
     6181 gtgcaggtat cgcacgcgat tacatcgttg cgccaactca ggataagtct cctttcatta
     6241 agactgctcc tacgtggtat ccggccaagc agctcccgga cggcttcaag atctacagaa
     6301 caggcgatct tgcatgctac gcatctgatg gcagtatagt gtgccttggc cgtatggact
     6361 cccaggtcaa gatcagagga cagcgtgttg agctgggtgc agtggagact catcttcgac
     6421 agcagatgcc tgatgatatg accatcgttg ttgaagctgt caagttctct gattcctcca
     6481 gtactacagt tttgacagcc ttcttgattg gtgcagggga gaagaacagt catatcttgg
     6541 accagcgtgc tacaagggag atcaatgcca agatggagca agttcttcct cgccattcca
     6601 tccccgcgtt ctacatctct atgaacaacc tcccccaaac agccactggt aaggtcgatc
     6661 gacgaaaact tcgtatcatg ggtagcaaga tactgagcca aaagacgcat tccaccccat
     6721 ctcagcagag tcaggccgcc atctcatctg gaaccgacac agagaccaag ctggagagca
     6781 tctggatcac aagcttggac ctcgagcccg gctctgcaaa catgtcagct acattcttcg
     6841 agatgggcgg aaactccatc atcgcaatca agatggtcaa catggcgcgc tccaatggca
     6901 tagagcttaa ggtctcggac atttaccaga accccacgct cgctggtctc aaggctattg
     6961 tcattggtac ttcgctgcca tacagcctta ttcccaaggt tacccgccaa ggccctgtta
     7021 gcgagcagtc ttatgcgcaa aacagaatgt ggttcctgga tcagttgtct gagggtgctt
     7081 catggtatct gattcctttc gctgtgcgca tgcgaggtcc ggtggatgtt gatgcgttga
     7141 cgcgtgcttt gctcgctctt gaacagcgtc atgagacact acgaactaca tttgagaacc
     7201 aagatggtgt tggagtccag atcatccatg atagactctc caaagagcta caagtcatcg
     7261 atgccttgga tggtgacgag ggtggtctca agacactcta caaagtagag accaccacat
     7321 tcgacatcac atccgaagca ggctggagct caaccctcat ccgcctcggc aaagacgatc
     7381 acattctgtc tatcgtcatg caccacatca tctccgacgg ctggtccatc gacgttctcc
     7441 gccgcgaact catccaactc tacgccgccg ctctgcaggg caaggatcct tcctccgcac
     7501 taacccccct acccatccag tacagcgact tcgccgtgtg gcagaagcag gaggcccaag
     7561 cagctgagca cgagaggcag ctccagtact ggaagaagca actcgcagat agttcacctg
     7621 ccaagatccc taccgacttc ccccgtccag atctcctgtc cggtgacgca ggcgttgtgc
     7681 ccgttgccat cgacggcgag ctgtatcaga aactaagggg cttctgcaac aagcacaaca
     7741 gcactgcctt ctccatcctg ctcgctgctt tccgcgcggc gcattaccgt ctcacagccg
     7801 ttgacgacgc cgtgatcggc atccccattg caaaccgtaa ccgctgggag ctggagaaca
     7861 tgattggttt ctttgtcaac acgcagtgta tgcgcatcgc cgttgacgag acggatacat
     7921 ttgagagtct ggtgcgccag gtcagatcta ccactacagc tgcgtttgcg cacgaggatg
     7981 tccccttcga gcgtgtcgtt tcagcgcttc agcctggcca tagagatctc tcgcgaacac
     8041 cgctggcaca gataatgttt gctgttcact cgcagaagga ccttggacgt ttcgagctgg
     8101 agggtatcca gtctgagcct atcgccagca aggcctacac cagattcgat gtcgagttcc
     8161 atctgttcca acaggcagac ggactgaagg gcagttgcaa ctttgccaca gatctcttca
     8221 agcccgaaac tatccagaat gttgtcagcg tgtttttcca gattctacgc catggccttg
     8281 accagcctga gacgtgtatc tcggttcttc cactgactga tggagtcgag gagcttcgca
     8341 ggttggatct gctggaaatc aagaggacta actaccctcg cgattcgagc gtggtagatg
     8401 tcttccgcga acaagctgcc gccaacccag aggttatcgc tgttaccgac tcatcttctc
     8461 gtctgactta tgcagagctg gacaataagt ctgagctgct ctcacgatgg cttcgacgac
     8521 gtaacttgac gccagagacg ctggtcagtg ttcttgctcc ccggtcttgc gagactatcg
     8581 ttgcctatgt tggtatcctc aaggcgaacc tggcgtatct tcctcttgac gtgaggtccc
     8641 cggtgactcg tatgaaggac atcttgtcga gcgtgtctgg aaacaccata gttcttatgg
     8701 gctctggggt agaggatcct ggctttgact taccgcaact agagctcgta cgcatcaccg
     8761 acactttcga tgagaccatc gaggacgtgc aagactctcc ccaaccgtct gccacaagcc
     8821 tcgcctacgt cgtcttcaca tccggttcaa ctggtaaacc caagggcgtc atgatcgagc
     8881 accgggccat tgtgcgtctc gtcaagagtg acaactttcc tggcttcccc tcccccgctc
     8941 gcatgtcaaa tgtcttcaac cctgccttcg acggagccat ctgggagatc aactggatgc
     9001 tcctgaacgg cggaacagtc gtctgcatcg actacctgac caccctggac ggcaaagagc
     9061 tcgctgctgt gttcgccaaa gagcgcgtca acgccgcctt cttcgcacct gcgatgctca
     9121 agctttacct cgttgatgcg cgcgaggctt tgaagaatct tgacttcctt attgttggag
     9181 gtgagaggtt cgataccaag gaagccgtgg aggccatgcc gcttgtgaga ggcaagattg
     9241 ccaatatcta tggtccgact gaggctggaa taatcagcac gtgctataac atccccaagg
     9301 atgaggctta caccaatggt gttcccattg gtggaagtat ctacaactct ggtgcctacg
     9361 tcatggatcc taaccagcaa cttgtcggcc ttggcgtcat gggagagctt gttgttaccg
     9421 gagacggtgt tggtcgaggc tacactaacc ccgagctcaa caagaaccgc ttcatcgaca
     9481 tcaccatcga gggcaagact ttcaaggctt accgtactgg tgaccggatg cgtgcacgag
     9541 taggcgacgg tctccttgag ttcttcggcc gcatggacaa ccagttcaag atccgcggca
     9601 accgtatcga agcaggggaa gttgagtctg ccatgctcag cctcaagaat gtccttaacg
     9661 ccgccattgt cgtccgcggg ggcggagaag atgaagggcc actcgagatg gtcggattca
     9721 tcgtcgcgga cgacaagaat gataccacgg aggaggaaga gacaggcaac caagttgagg
     9781 gctggcagga ccatttcgag agtggtatgt actcggatat cagcaccgcc gtggaccaat
     9841 ctgccattgg aaacgacttt aagggctgga cttctatgta cgatggtaag gatatcgaca
     9901 agggcgagat gcaggagtgg ttggacgacg ctattcacac cctgcataac ggccagatcc
     9961 ctcgcgatgt cctcgagatc ggtaccggta gtggtatgat cctgttcaac ctcaacccgg
    10021 gcctcaacag ctacgttggt cttgatccat ccaagtcagc agtcgagttc gtcaacagag
    10081 ccgtcgagtc ctctcccaag ttcgcaggaa aagccaaggt ccacgtcggc atggccacag
    10141 acgtcaacaa actcggcgaa gtacaccccg atctcgtggt cttcaactcc gttgttcaat
    10201 acttccccac acccgagtat ctcgccgagg tcatcgatgg cctcattgcc atccccagcg
    10261 tcaagcgcat cttccttggc gatatcagat catatgctac caacggacac ttcctcgccg
    10321 cacgcgccat ccacacgctc ggcaccaata acaacgccac caaggatagg gtgcgccaga
    10381 agatccagga gctggaggat cgagaggaag agtttctcgt tgagcctgcc ttcttcacca
    10441 ctctgaagga gcgacgtcca gatgttgtca agcatgttga gatcatcccc aagaacatga
    10501 aggccaccaa cgaactcagc gcctatcgct acacggctgt tgtgcatctg cgggatgaaa
    10561 cggacgaacc tgtgtatcat attgagaagg atagctgggt tgactttgag gcgaagcaga
    10621 tggacaagac ggctcttctt gaccacctgc gcctctccaa ggatgctatg agtgtggcgg
    10681 ttagcaacat cacctacgcc cacactgcct ttgaacgtcg tatcgttgag tctctcgatg
    10741 aggatagcaa ggatgacacc aagggtacac tcgatggtgc agcctggctc tcagcagttc
    10801 gctccgaagc cgaaaaccgc gcctcactca ccgtccccga catcctggag atcgccaaag
    10861 aggctggttt ccgagttgaa gtcagcgctg ctcgccagtg gtcccaaagt ggtgctttag
    10921 acgcagtctt ccaccacttt ccaccctcca gcactgaccg cactctaatc cagttcccca
    10981 cggacaacga gcttcgatca tcactcaccc tcgccaaccg ccctctccag aagctgcagc
    11041 gccgtcgtgc cgctctgcaa gtccgcgaga agctccagac gctggtcccg tcttacatgg
    11101 ttcctccgaa tatcgtggtg ctggacacga tgcctctcaa tactaacggc aagatcgaca
    11161 gaaaggagct tacgcgtaga gcacgaacac tgccgaagca gcagactgcg gcgcctgtgc
    11221 cggacttccc tatctctgat atcgagatca cgctgtgcga ggaggcaact gaggtctttg
    11281 gaatgaaggt tgaaatcagc gatcacttct tccagctcgg cggtcactct ctcctcgcta
    11341 cgaaactcat ttctcgcatc cagcaccgtc tccatgtgcg ggttactgtg aaggacgtat
    11401 tcgacagccc tgtctttgcc gatctggcag tcatcatccg tcaaggactt gctatgcaga
    11461 accctgttgc tgaaggacag gacaagcaag gctggtcctc gagagttgcc cctcgtacag
    11521 aagtcgagaa gatgctgtgc gaggagttcg cagctggtct tggtgtcccg gttggtatca
    11581 ctgacaactt cttcgatctc ggtggtcact cgctcatggc tactaagcta gctgtgcgaa
    11641 ttggccgtcg ccttgatacc gccatcacag tcaaggacat cttcgactac cctgtgcttt
    11701 tccaattggc gaagaagttg gagtcttcgc attccaagag ctacgaggag tctggcgacg
    11761 atatccagat ggccgattac actgcattcc agctcctcga tctggaagac ccccaagact
    11821 ttgttcagtc ccagattcgg cctcaactgg actcctgcta cggcaccatc caggatgtct
    11881 acccgtctac gcagatgcaa aaggccttcc tcttcgatcc cacgaccggc gagccccgag
    11941 gtcttgtgcc tttctatatc gacttcccca gcaatgcaga tgccgagacc ctcaccaagg
    12001 ctatcggagc tctagttgac aagctcgata tgttcagaac tgtcttcctg gaggccgcag
    12061 gcgatctgta ccaagttgtc gttgagcacc tcaacttgcc tattgagacc atcgagactg
    12121 agaagaacgt caacactgca accggtgact acctggatgt tcatggaaag gaccctgtcc
    12181 gtctaggcca cccgtgcatc caattcgcca tcctgaagac tgcctcctct gtacgtgttc
    12241 tcctgcgaat gtcccacgct ctgtatgatg gtttgagttt tgagtacatc gtccgtggtc
    12301 tccacgttct ctacagcggt agaaacctcc ccccaccaac acagtttgcg cgatacatgc
    12361 agtatgctgc acacagtcgt gaagaaggtt atcccttctg gcgcgaggtt ctgcagaacg
    12421 cgcccatgac agttctacac gacaccaata acggtatgtc tgagcaagag atgccagcct
    12481 ccaaggcggt acacctgtca gaggtcgtca acgttccagc gcaagctatt cgaaacagta
    12541 ccaacaccca agcgaccgtc ttcaacaccg cctgtgccct tgtcctagcc aaggaatccg
    12601 gctcacagga tgtcgtcttc ggccgtattg tctctggtcg acaaggtcta ccagtcgtct
    12661 ggcaggacat catcggcccc tgcacaaacg ccgtgcccgt ccacgcacgc gtcgacgatg
    12721 gaaaccccca acgcatcatc cgcgacctac gcgaccaata cctccgcact ctgcccttcg
    12781 aatcgcttgg cttcgaggaa atcaagcgta actgcacgga ctggcccgaa gaattgacca
    12841 acttctctgt ctgcgtgacg taccacaact tcgagtacca ccccgagagc gaagttgaca
    12901 accaaaaggt tgagatggga gttttggcaa agtatgttga gttgagtgaa aacgagccgc
    12961 tgtacgatct tgctattgcg ggagaggttg aggcggatgg agttaacctg aaggtcactg
    13021 ttgttgcaaa ggcaaggctt tacaatgagg cgaggattag acatgtgctt gaggaagttt
    13081 gcaaaacttt caatggtttg aacgaggctt tgtaagttta aacagtagat gccgaccggg
    13141 atccacttaa cgttactgaa atcatcaaac agcttgacga atctggatat aagatcgttg
    13201 gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat aagatacgtt
    13261 catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg tcaacaagaa
    13321 taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc attaatgcat
    13381 tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa tagcttagca
    13441 gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc aagagaccta
    13501 cgagactgag gaatccgctc ttggctccac gcgggcgcgc ctcggtcgct cactgttcct
    13561 ttacggatat gtgtactccg gcaagccgcg gggtcgcggt cgtttgtacg gcagcttcta
    13621 cttgcttgca cagggagctc tctggggctt gacgtctttt ggagttgcga gggagttgat
    13681 ttcctacttc taagtttgga ctgaatccgt ggtgtgattg aggtgattgg cgatgtttgg
    13741 ctataccagc tatatgtaat aatctctact gtatactact attcaacgca ttttactatg
    13801 cgtgctgcta gggtcggcaa tgacaatggc aatctgactg acgtggtcta tttctccatg
    13861 tgcagcaggg aatacgagct ccaatggacc tcgggagtgg cacagtcaat ggcaaggaaa
    13921 ctccgccttt gcaggtgtgg ctgaacccca cgggtcggag gcggagcaat ccacccccga
    13981 tgtggctggt gcgtggaggg gctcgcgatg attttactga gcttgctttt cttgtcgaca
    14041 ttgaacattg tccttggtct tccttcagat ttaagggtca gtcactgcta catttctcag
    14101 tagtatccgc gcacgtctct ggatttacga atcagggtcc accagtcgaa acttcgaact
    14161 actctcatta tacaatcctc tttccattcc cgcattaacc cctccatcaa caccatgtcc
    14221 tccaagtcgc aattgaccta cactgcccgt gccagcaagc atcccaatgc tctggcgaag
    14281 aggctgttcg agattgccga ggccaagaag accaatgtga ctgtctcggc tgacgttacc
    14341 accactaagg agctactaga tcttgctgac cgtaggccga cccgctactc tgcctgatta
    14401 tgctgcatgc aaacttatta acggtgatac cggactgcag gtctcggtcc ctacattgcc
    14461 gtgatcaaaa cccacatcga tatcctctct gatttcagca acgagaccat tgagggactt
    14521 aaggctctcg cgcagaagca caactttctc atcttcgagg accgcaagtt cattgacatc
    14581 ggcaacacgg tccagaagca ataccacggc ggtaccctcc gtatctcgga atgggcccac
    14641 atcatcaact gcagcattct ccctggtgag ggtatcgtcg aggctctcgc tcagacggcg
    14701 tctgcaccgg acttcgcata cggccccgaa cgcggtctgt tgatcttggc agagatgacc
    14761 tctaagggct ccttggctac cggccagtac actacttcct cggtcgatta tgcccggaaa
    14821 tacaagaact tcgttatggg attcgtgtcg acgcgcgcgt tgggtgaggt gcagtcggaa
    14881 gtcagctctc cttcggatga ggaggacttt gtggtcttca cgactggtgt gaacatttct
    14941 tccaagggag ataagcttgg tcagcagtac cagacgcccg gatcggctat cggccggggt
    15001 gctgacttca ttatcgcggg tcgcggtatc tacgccgcgc cggatcgatc cggtgcaggc
    15061 tgcgcaacag tatcagaagg aggggtggga agcctacctg gcccgtgtcg gcggaaacta
    15121 atactataaa aggaggatcg aagttctgat ggttatgaat gatatagaaa tgcaacttgc
    15181 cgcaacggat acggaagcgg aaacggacca atgtcgagca cgggtagtca gactgcggca
    15241 tcggatgtcc aaacggtatt gatcctgcag gctactatgg tgtggcacaa ggatcaatgc
    15301 ggtacgacga tttgatgcag ataagcaggc tgcgaagtag taactcttgc gtagagaaaa
    15361 tggcgacggg tggctgataa gggcggtgat aagcttaatt gtcatcgcag ataagcactg
    15421 ctgtcttgca tccaagtcag cgtcagcaga aatacgggac ttccgaaagt atatggcaaa
    15481 attaaagaac ttgactctcc agcaatgttt tgccctgacc gtcgctaaaa cgttactacc
    15541 cctatacccg tctgtttgtc ccagcccgag gcattaggtc tgactgacag cacggcgcca
    15601 tgcgggcttg ggacgccatg tccgtcgcgt gataagggtt gatccatgca gctactatcc
    15661 ttccatcgtt ccattcccat ccttgtccta tctccatcct tgaaacttta ctagtttagt
    15721 tggatgctcg agcttgctct cggctactcc gtcggcgcgc cttgcggccg ccaccgcggt
    15781 ggagctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
    15841 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    15901 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    15961 gcctgaatgg cgaatggaaa ttgcactgta gcggcgcatt aagcgcggcg ggtgtggtgg
    16021 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
    16081 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
    16141 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
    16201 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
    16261 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
    16321 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
    16381 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccgtgg
    16441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    16501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    16561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    16621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    16681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    16741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    16801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    16861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    16921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    16981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    17041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    17101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    17161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    17221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    17281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    17341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    17401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    17461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    17521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    17581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    17641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    17701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    17761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    17821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    17881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    17941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    18001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    18061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    18121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    18181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    18241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    18301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    18361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    18421 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    18481 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    18541 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    18601 gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgaggtc
    18661 gacggtatcg ataagcttcg gagaatatgg agcttcatcg aatcaccggc agtaagcgaa
    18721 ggagaatgtg aagccagggg tgtatagccg tcggcgaaat agcatgccat taacctaggt
    18781 acagaagtcc aattgcttcc gatctggtaa aagattcacg agatagtacc ttctccgaag
    18841 taggtagagc gagtacccgg cgcgtaagct ccctaattgg cccatccggc atctgtaggg
    18901 cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag
    18961 gagctggcca gcggcgcaga ccgggaacac aagctggcag tcgacccatc cggtgctctg
    19021 cactcgacct gctgaggtcc ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc
    19081 ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca acatttgttg ccatattttc
    19141 ctgctctccc caccagctgc tcttttcttt tctctttctt ttcccatctt cagtatattc
    19201 atcttcccat ccaagaacct ttatttcccc taagtaagta ctttgctaca tccatactcc
    19261 atccttccca tcccttattc ctttgaacct ttcagttcga gctttcccac ttcatcgcag
    19321 cttgactaac agctaccccg cttgagcaga catcaccgaa ttcaccatgt ctagactgga
    19381 caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct
    19441 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
    19501 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
    19561 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
    19621 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
    19681 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
    19741 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
    19801 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
    19861 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
    19921 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
    19981 cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
    20041 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
    20101 gctccccggg taactaagta aggatccact agtacagcag aagaatctct ctccgctgtt
    20161 gcttcagtgt ctgccatgca ttaacttcat cctactgtcc tacccgcagt acccattcac
    20221 atttgcggca gataccaggt tgttttctat tccctcggtt cttcagttct tcagatatta
    20281 cattactcga gaaattggcg gcgatgcagg agtttggttg gttttaattg tttcagtctc
    20341 cttgacctga tttgtatgac atgcaatgtt tcgacggaag actatctcgg tgaatatacg
    20401 tcatgaatca tgcggccgcg cgtatcacga ggccctttcg tcttcactcg agtttaccac
//
