LOCUS       sequence-226502-       15502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15502)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-151
FEATURES             Location/Qualifiers
     source          1..15502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..108
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             152..811
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(218..237)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      876..962
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1073..1238
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(1671..2621)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2669..2891
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     3574..3593
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            3631..3740
                     /note="incP origin of transfer"
     primer_bind     3763..3782
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(3911..4702,4703..4771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4534..4553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4772..4863)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(4882..4909)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5001..5087)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5240..5257
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(11948..12232)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12006..12025)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12259..13137
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             13629..14423
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     GQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDVGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             join(14786..14788,14789..15502)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(14828..14849)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(15089..15108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     15436..15457
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
       61 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagctagctt
      121 ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      181 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      241 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      301 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      361 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      421 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
      481 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
      541 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
      601 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
      661 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
      721 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
      781 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
      841 aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag gctcagtcga
      901 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      961 atccgccgct aggagcttgc ggccgccagg ggatctgatt aattaaaacg tggcttacta
     1021 ggatccgata tcatttaaat ctagggataa cagggtaata ctagtgtcga ccaaccagat
     1081 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
     1141 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
     1201 ccacccctcc cagttcccaa ctattttgtc cgcccacagg ctgaaagcgc tatttcttcc
     1261 agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct
     1321 ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa
     1381 caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg gtttcacctg
     1441 ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca tggtgaacag
     1501 ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
     1561 catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
     1621 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     1681 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     1741 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     1801 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     1861 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     1921 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     1981 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     2041 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     2101 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     2161 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     2221 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     2281 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     2341 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     2401 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgaacgtat
     2461 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     2521 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     2581 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     2641 tgattttaat cactatacca attgagatgg gctagtcaat gataattact agtccttttc
     2701 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     2761 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     2821 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     2881 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     2941 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     3001 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     3061 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     3121 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     3181 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
     3241 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
     3301 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
     3361 tcttactgtc ggggatcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     3421 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     3481 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     3541 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     3601 aaccgcacct gtggcgccgg tgatgccgcc gggcaggata ggtgaagtag gcccacccgc
     3661 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
     3721 tgctctgcga ggctggccgg cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3781 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3841 cgcgcttaat gcgccgctac agggcgcgta aatcaatcta aagtatatat gagtaaactt
     3901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4201 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     4261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4501 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     4561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4861 aaaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttaatttga
     4921 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg
     4981 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     5041 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
     5101 ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
     5161 gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat tctgttttat
     5221 cagaccgctt ctgcgttctg atttaatctg tatcaggctg aaaatcttct ctcatccgcc
     5281 aaaacagcca agcttgcatg cctgcaggtc gactctagag gatcccctaa aaataatgac
     5341 cgagctagct ctaactgtgg gatgtgataa cgagtcttgc tatatggtgt ttcagcttcg
     5401 taaataaatg aactctgcgc tccatccttg ttgcgtacgg aagcaaagga cgagagatta
     5461 ggaaaatcga ttattttggc tcgttgaaga tccgatgctt caaactcaat cacacgatcc
     5521 gccttattta tttgctttgt tgtactgtta accaccttag ctcgacttaa taaaggcaaa
     5581 tgagtaaatc gagtccattt ccgttcttgc attggattaa accaaacaaa aatacgccac
     5641 tccttatgac ccgagttacg gaataaatga ccaatatgta caacctgcac attgtcatta
     5701 aatgtagcta gcctaaccac tcactcttcc ccataaacca tttccaagaa tctttgtgct
     5761 tcctcagtta tccgctcttt agataacgtt gcacttctta ataatcgcca gcgcctaagc
     5821 tcaacatctt cctgtttaag cttaataaaa gcttggctaa tccgtctaat ttgataatct
     5881 tccacactct ctgagtaacg ctttaagcaa agcgctacca aaggcagttt ctgtagattt
     5941 tttgcaagag aggttccgtt aggagtttgc tttaacagcc agctcgatgt cgctcttggg
     6001 tgatcaaggc tactatctag acgctttatg attcttaata gttgtcgtac agcaattcga
     6061 tctctttggt tccaatcaac tctaggggcg ggtgccagac gctcttgttg atgctgttga
     6121 ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc aagcgtatag caccccaccc
     6181 tctaaagact gtcttgccgc cttaattcct tggtatttat gcactagttg ctgccagtct
     6241 ttatgcttaa cagataaatc ttcagagtta ggttgcacag actggctaac aggtcttgtc
     6301 gttatagagt gctcagtaag agcacttgcc tgctgtagcg cttctataac cgttagtttt
     6361 ggcaataagg cttgccacac aatactatgc tgtaaataac taaaggcttt tctatgctta
     6421 cggaatatac ttttcagcca acacgtgtcc ttgttctctg ccaactttaa atccagtttc
     6481 tctagtgcct catcactaaa agtctgcctc actctctccg ccaccaagtc atgacgaatg
     6541 tgcttgcttt tggttagccc tagatcctgc gctaagcgct gataaaacag cgtccactgc
     6601 tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta acagaggggc tatataagca
     6661 gctagtgctg ttaattgaga tagggagtct ttggggtagt ctgaaagcag ctcagtatga
     6721 cccaaagccc aaaattgatg tcggtgatca tctacagctc tatcaaagaa gactaaagca
     6781 ccgtgttttg gacaatatgg caaagcgggc aaataccaat ctcgttgcca aaaggcttcc
     6841 ccatacctat ttagctgaag agcaacgcaa tcagggcagt atctaaagcg gttatcgctc
     6901 ttaactctag aagcagcgac tcctagcatt aaatgcaccg caccttgcgc ttggtactcc
     6961 attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa acggagcata taaagggaat
     7021 aaggtatgct cataaataag ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt
     7081 gctatcacac ctaaatgcga gggcagacct aaggtagcga ccaccttgcg gttgccatac
     7141 acctcatcca acagctgctt aggactaaca atcccttgat aaacgcctgc ccgtgcaata
     7201 gtgctataaa tcagctcatt cgagtacgga acaggaaagt ttctcatggc taacctgctt
     7261 gtttaaataa gctcgccata tccactatta cccctttccc ttttaaccgt tcatgcatgg
     7321 ttttttcagg ttggcgttgt gaatagatat aacgtaaatc cgtatcaggc aagctatccc
     7381 agtcgctggg cttgactacc tttatagccg aaaccttttt actcttggag ggcttttcta
     7441 gttctgacac taccgtttcg ccttccatca accactgcaa aacaagaggc agtaactttt
     7501 gtcttgtcat cgttggattc tggctaaacg cttttttaat agtgggaatt aacaggcttg
     7561 aatcgtaatc ctctttcagc atcagatata aatgacgctg atcttcggta tctaactctt
     7621 gaagggcttt ttcttctggt gtttgttctt gtatcgctgc gatatctagc tgaagttgga
     7681 ttaaccgttt atcaatctcg ggaacgacta gatcagaata acgagcaatg cgttctggga
     7741 tacccgagcg taatgcctct agcatggggt gcacaggctt taactcatct tgatacactt
     7801 gccgcaataa accagcggta atacgctcat tgcctaaagc tagcgcacgg agctgagcga
     7861 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     7921 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     7981 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     8041 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     8101 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     8161 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     8221 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     8281 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     8341 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     8401 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     8461 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     8521 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     8581 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     8641 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     8701 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
     8761 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
     8821 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
     8881 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
     8941 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
     9001 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
     9061 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
     9121 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
     9181 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
     9241 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
     9301 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
     9361 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
     9421 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
     9481 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
     9541 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
     9601 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
     9661 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
     9721 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
     9781 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
     9841 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
     9901 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
     9961 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
    10021 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
    10081 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
    10141 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
    10201 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
    10261 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
    10321 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
    10381 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
    10441 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
    10501 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
    10561 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
    10621 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
    10681 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
    10741 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
    10801 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
    10861 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
    10921 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
    10981 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
    11041 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
    11101 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
    11161 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
    11221 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
    11281 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
    11341 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
    11401 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
    11461 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
    11521 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
    11581 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
    11641 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
    11701 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
    11761 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    11821 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    11881 tacaactatt ctgtctagtt taagacttgg gtaccgagct cgaattcgct agcccaaaaa
    11941 aacgggtatg gagaaacagt agagagttgc gataaaaagc gtcaggtagg atccgctaat
    12001 cttatggata aaaatgctat ggcatagcaa agtgtgacgc cgtgcaaata atcaatgtgg
    12061 acttttctgc cgtgattata gacacttttg ttacgcgttt ttgtcatggc tttggtcccg
    12121 ctttgttaca gaatgctttt aataagcggg gttaccggtt tggttagcga gaagagccag
    12181 taaaagacgc agtgacggca atgtctgatg caatatggac aattggtttc ttctctgaat
    12241 ggcgggagta tgaaaagtat ggctgaagcg caaaatgatc ccctgctgcc gggatactcg
    12301 tttaatgccc atctggtggc gggtttaacg ccgattgagg ccaacggtta tctcgatttt
    12361 tttatcgacc gaccgctggg aatgaaaggt tatattctca atctcaccat tcgcggtcag
    12421 ggggtggtga aaaatcaggg acgagaattt gtttgccgac cgggtgatat tttgctgttc
    12481 ccgccaggag agattcatca ctacggtcgt catccggagg ctcgcgaatg gtatcaccag
    12541 tgggtttact ttcgtccgcg cgcctactgg catgaatggc ttaactggcc gtcaatattt
    12601 gccaatacgg ggttctttcg cccggatgaa gcgcaccagc cgcatttcag cgacctgttt
    12661 gggcaaatca ttaacgccgg gcaaggggaa gggcgctatt cggagctgct ggcgataaat
    12721 ctgcttgagc aattgttact gcggcgcatg gaagcgatta acgagtcgct ccatccaccg
    12781 atggataatc gggtacgcga ggcttgtcag tacatcagcg atcacctggc agacagcaat
    12841 tttgatatcg ccagcgtcgc acagcatgtt tgcttgtcgc cgtcgcgtct gtcacatctt
    12901 ttccgccagc agttagggat tagcgtctta agctggcgcg aggaccaacg tatcagccag
    12961 gcgaagctgc ttttgagcac cacccggatg cctatcgcca ccgtcggtcg caatgttggt
    13021 tttgacgatc aactctattt ctcgcgggta tttaaaaaat gcaccggggc cagcccgagc
    13081 gagttccgtg ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt gtcataattg
    13141 gtaacgaatc agacaattga cggcttgacg gagtagcata gggtttgcag aatccctgct
    13201 tcgtccattt gacaggcaca ttatgcatcg atgataagct gtcaaacatg agcagatcct
    13261 ctacgccgga cgcatcgtgg cctgtgggcg gacaataaag tcttaaactg aacaaaatag
    13321 atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc
    13381 cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt
    13441 tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gggtcgacgc ggccgtggcg
    13501 cgcctcctag gtgctcgagt ggcggccgct attgacccat aaactgccag gaattgggga
    13561 tcggattcga gctaagaatt cccttggggt atctttaaat actgtagaaa agaggaagga
    13621 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
    13681 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
    13741 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
    13801 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
    13861 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
    13921 gctcggaaga gtatgaagat ggacaaagcc ctgaaaagat tatcgagctg tatgcggagt
    13981 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
    14041 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
    14101 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
    14161 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
    14221 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
    14281 ggtatgacat tgccttctgc gtccggtcga tcagggagga tgtcggggaa gaacagtatg
    14341 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
    14401 ttttactgga tgaattgttt tagagctcgc acgctgccgc aagcactcag ggcgcaaggg
    14461 ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
    14521 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
    14581 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
    14641 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
    14701 gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac
    14761 aggatactag tggaggaaga aaaaaatgag caagggcgag gagctgttca ccggggtggt
    14821 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
    14881 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
    14941 gctgcccgtg ccctggccca ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc
    15001 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
    15061 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
    15121 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
    15181 ggacggcaac atcctggggc acaagctgga gtacaactac tttagcgaca acgtctatat
    15241 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
    15301 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
    15361 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
    15421 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
    15481 catggacgag ctgtacaagt aa
//
