LOCUS       sequence-226519-        9591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  broad-host range plasmid to constitutively express fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE161
FEATURES             Location/Qualifiers
     source          1..9591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(64..66,67..780)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(106..127)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(367..386)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     714..735
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      794..888
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             932..1591
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(998..1017)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1656..1742
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2081..2875)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2192..2211)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2802..2821
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      4355..5126
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             5127..5789
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     6483..6505
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6497..6514
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6498..6514
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6524..6543
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6524..6542
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      6585..6671
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6768..6854
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6951..7037
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7134..7220
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7322..8521)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 atctgatggc gcaggggatc aagatctgat caagagacag gatactagtg gaggaagaaa
       61 aaaatgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      121 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      181 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      241 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      301 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      361 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      421 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      481 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
      541 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      601 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      661 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      721 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      781 ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
      841 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagctagctt
      901 ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      961 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     1021 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     1081 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     1141 tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     1201 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     1261 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     1321 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     1381 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1441 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1501 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1561 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
     1621 aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag gctcagtcga
     1681 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     1741 atccgccgct aggagcttgc ggccgccagt gtgatggata tctgcataaa aactgttgta
     1801 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1861 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatgggg gtgggcgaag
     1921 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     1981 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     2041 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     2101 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     2161 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     2221 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     2281 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     2341 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     2401 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     2461 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     2521 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     2581 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     2641 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     2701 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     2761 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     2821 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     2881 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     2941 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     3001 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
     3061 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
     3121 agtagctgac attcatccca ggtggcactt ttcggggaaa tgtgcgcgcc cgcgttcctg
     3181 ctggcgctgg gcctgtttct ggcgctggac ttcccgctgt tccgtcagca gcttttcgcc
     3241 cacggccttg atgatcgcgg cggccttggc ctgcatatcc cgattcaacg gccccagggc
     3301 gtccagaacg ggcttcaggc gctcccgaag gtctcgggcc gtctcttggg cttgatcggc
     3361 cttcttgcgc atctcacgcg ctcctgcggc ggcctgtagg gcaggctcat acccctgccg
     3421 aaccgctttt gtcagccggt cggccacggc ttccggcgtc tcaacgcgct ttgagattcc
     3481 cagcttttcg gccaatccct gcggtgcata ggcgcgtggc tcgaccgctt gcgggctgat
     3541 ggtgacgtgg cccactggtg gccgctccag ggcctcgtag aacgcctgaa tgcgcgtgtg
     3601 acgtgccttg ctgccctcga tgccccgttg cagccctaga tcggccacag cggccgcaaa
     3661 cgtggtctgg tcgcgggtca tctgcgcttt gttgccgatg aactccttgg ccgacagcct
     3721 gccgtcctgc gtcagcggca ccacgaacgc ggtcatgtgc gggctggttt cgtcacggtg
     3781 gatgctggcc gtcacgatgc gatccgcccc gtacttgtcc gccagccact tgtgcgcctt
     3841 ctcgaagaac gccgcctgct gttcttggct ggccgacttc caccattccg ggctggccgt
     3901 catgacgtac tcgaccgcca acacagcgtc cttgcgccgc ttctctggca gcaactcgcg
     3961 cagtcggccc atcgcttcat cggtgctgct ggccgcccag tgctcgttct ctggcgtcct
     4021 gctggcgtca gcgttgggcg tctcgcgctc gcggtaggcg tgcttgagac tggccgccac
     4081 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
     4141 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
     4201 actcaatgct tgagtatact cactagactt tgcttcgcaa agtcgtgacc gcctacggcg
     4261 gctgcggcgc cctacgggct tgctctccgg gcttcgccct gcgcggtcgc tgcgctccct
     4321 tgccagcccg tggatatgtg gacgatggcc gcgagcggcc accggctggc tcgcttcgct
     4381 cggcccgtgg acaaccctgc tggacaagct gatggacagg ctgcgcctgc ccacgagctt
     4441 gaccacaggg attgcccacc ggctacccag ccttcgacca catacccacc ggctccaact
     4501 gcgcggcctg cggccttgcc ccatcaattt ttttaatttt ctctggggaa aagcctccgg
     4561 cctgcggcct gcgcgcttcg cttgccggtt ggacaccaag tggaaggcgg gtcaaggctc
     4621 gcgcagcgac cgcgcagcgg cttggccttg acgcgcctgg aacgacccaa gcctatgcga
     4681 gtgggggcag tcgaagggcg aagcccgccc gcctgccccc cgagcctcac ggcggcgagt
     4741 gcgggggttc caagggggca gcgccacctt gggcaaggcc gaaggccgcg cagtcgatca
     4801 acaagccccg gaggggccac tttttgccgg agggggagcc gcgccgaagg cgtgggggaa
     4861 ccccgcaggg gtgcccttct ttgggcacca aagaactaga tatagggcga aatgcgaaag
     4921 acttaaaaat caacaactta aaaaaggggg gtacgcaaca gctcattgcg gcaccccccg
     4981 caatagctca ttgcgtaggt taaagaaaat ctgtaattga ctgccacttt tacgcaacgc
     5041 ataattgttg tcgcgctgcc gaaaagttgc agctgattgc gcatggtgcc gcaaccgtgc
     5101 ggcaccccta ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag
     5161 ccaagaacaa gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg
     5221 ggcttattgc gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg
     5281 gccaccagaa cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt
     5341 tgcggacggt ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga
     5401 agctcaacgg ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc
     5461 agccccgcga ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg
     5521 accaggacga atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg
     5581 gcgagcagca actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg
     5641 gcatggaacc agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc
     5701 agcgcctgcc gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc
     5761 cgacacgggt cacgctgccg cgccggtagc acttgggttg cgcagcaacc cgtaagtgcg
     5821 ctgttccaga ctatcggctg tagccgcctc gccgccctat accttgtctg cctccccgcg
     5881 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg
     5941 ctaacggatt caccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
     6001 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
     6061 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     6121 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     6181 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     6241 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac ggcctattgg ttaaaaaatg
     6301 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca
     6361 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     6421 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     6481 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
     6541 gggcgaattg gagctggggc cgcaattccc aattccccag gcatcaaata aaacgaaagg
     6601 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     6661 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     6721 gggcaggacg cccgccataa actgccagga attaattccc caggcatcaa ataaaacgaa
     6781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     6841 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     6901 ggcgggcagg acgcccgcca taaactgcca ggaattaatt ccccaggcat caaataaaac
     6961 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     7021 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     7081 ggtggcgggc aggacgcccg ccataaactg ccaggaatta attccccagg catcaaataa
     7141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     7201 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
     7261 gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttggggatcg gattcgagct
     7321 ctcagcgatc ggctcgttgc cctgcgccgc tccaaagccc gcgacgcagc gccggcaggc
     7381 agagcaagta gagggcagcg cctgcaatcc atgcccaccc gttccacgtt gttatagaag
     7441 ccgcatagat cgccgtgaag aggaggggtc cgacgatcga ggtcaggctg gtgagcgccg
     7501 ccagtgagcc ttgcagctgc ccctgacgtt cctcatccac ctgcctggac aacattgctt
     7561 gcagcgccgg cattccgatg ccacccgaag caagcaggac catgatcggg aacgccatcc
     7621 atccccgtgt cgcgaaggca agcaggatgt agcctgtgcc gtcggcaatc attccgagca
     7681 tgagtgcccg cctttcgccg agccgggcgg ctacagggcc ggtgatcatt gcctgggcga
     7741 gtgaatgcag aatgccaaat gcggcaagcg aaatgccgat cgtggtcgcg tcccagtgaa
     7801 agcgatcctc gccgaaaatg acccaaagcg cggccggcac ctgtccgaca agttgcatga
     7861 tgaagaagac cgccatcagg gcggcgacga cggtcatgcc ccgggcccac cggaacgaag
     7921 cgagcgggtt gagagcctcc cggcgtaacg gccggcgttc gcctttgtgc gactccggca
     7981 aaaggaaaca gcccgtcagg aaattgaggc cgttcaaggc tgccgcggcg aagaacggag
     8041 cgtgggggga gaaaccgccc atcagcccac cgagcacagg tcccgcgacc atcccgaacc
     8101 cgaaacaggc gctcatgaag ccgaagtgcc gcgcgcgctc atcgccatca gtgatatcgg
     8161 caatataagc gccggctacc gccccagtcg ccccggtgat gccggccacg atccgcccga
     8221 tatagagaac ccaaaggaaa ggcgctgtcg ccatgatggc gtagtcgaca gtggcgccgg
     8281 ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc cgacagcgcg cccagcacag
     8341 gtgcgcaggc aaattgcacc aacgcataca gcgccagcag aatgccatag tgggcggtga
     8401 cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag caccggcata atcaggccga
     8461 tgccgacagc gtcgagcgcg acagtgctca gaattacgat caggggtatg ttgggtttca
     8521 cgtctggcct ccggaccagc ctccgctggt ccgattgaac gcgcggattc tttatcactg
     8581 ataagttggt ggacatatta tgtttatcag tgataaagtg tcaagcatga caaagttgca
     8641 gccgaataca gtgatccgtg ccgccctgga cctgttgaac gaggtcggcg tagacggtct
     8701 gacgacacgc aaactggcgg aacggttggg ggttcagcag ccggcgcttt actggcactt
     8761 caggaacaag cgggcgctgc tcgacgcact ggccgaagcc atgctggcgg agaatcatac
     8821 gcattcggtg ccgagagccg acgacgactg gcgctcattt ctgatcggga atgcccgcag
     8881 cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc atccatgccg gcacgcgacc
     8941 gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc ttcctctgcg aggcgggttt
     9001 ttcggccggg gacgccgtca atgcgctgat gacaatcagc tacttcactg ttggggccgt
     9061 gcttgaggag caggccggcg acagcgatgc cggcgagcgc ggcggcaccg ttgaacaggc
     9121 tccgctctcg ccgctgttgc gggccgcgat agacgccttc gacgaagccg gtccggacgc
     9181 agcgttcgag cagggactcg cggtgattgt cgatggattg gcgaaaagga ggctcgttgt
     9241 caggaacgtt gaaggaccga gaaagggtgc taagaaacca ttattatcat gaagctcgca
     9301 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     9361 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     9421 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     9481 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     9541 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag g
//
