LOCUS       sequence-226570-        3390 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter paf from Penicillium chrysogenum domesticated into pUPD2 
            with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid 
            modular DNA assembly for A. tumefaciens-mediated genetic 
            transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB029
FEATURES             Location/Qualifiers
     source          1..3390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      1327..1384
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1408..1425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1579..2167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1659..1678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2350..2444)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2465..3124)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3039..3058
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3286..3304
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3308..3351)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3354..3375
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggaggaattc agagagcttt tcgtacgaag tgcgtaatgt acgtagcatt ttatggtagc
       61 atgcaaagca cattttgctg caaccccaat ttaatgcggt cctgctcaat aattgatctg
      121 cactaaggcc ttggcgatgg ggccagaaaa gggttgttca gtggtgtgta ctccgtaatg
      181 gtcaagccga tttcgagaat gaccgtagtg ttcattcatc agtgcgatat taaatcagtt
      241 agctactcta tctgaaagct aataaatttc tttaccacta acaatactct tctctgactg
      301 aaagtacctt ttccactccc ctcatacttc atgttttaag ctcaaccgta ggaaagcctg
      361 tatatcttaa aagatttgga tttactcttc cagcgcttac tgtctgctct ttcggccgag
      421 cgaaccttgg cagtatgatc ggactatgta ctttgttaca caaaaggaga agcggggctg
      481 ccactgagga caacccctgt tcaagggcta gcatcccgct gtaagcccac ccatcccacc
      541 ttgaagtatg caacttttga ccgcctagac catgtgagct tatgttactg aaatactacc
      601 cgcgaatcat ttcctaattt gctttggctc gaatccaccc cagccctacg taacacaacc
      661 gggagctgcc ttacagcttg gctgtatcac agtatcacat agatacatac atagtatagt
      721 gcctttgcct tttcgaccta taagcatccg ccatatgcta aaccttctca tataccaaca
      781 ttttggattt ggagatcatt tcctagtgaa acaactttat caaatgcaat gcagccatcg
      841 tcctttgcag atccgagtgg cccagtcacc gtgtcaacgt gtcagccgtt ttctctgctt
      901 tttaggaaat gattaccact aggtaagccc aaaaatatct tcctggtaaa caagtagtgc
      961 atcttacccc ggaggctgaa gcaggtaagg gattttggag agtccccacc cgtaagaata
     1021 taccagccaa gaggtccagt atcctgaagt atgtgaggca ttaatgtcat tggagaagtc
     1081 atgcaatcca taagctgcca cccccaagat gactgcattg gacctgagca ttgtatgtgt
     1141 cacctttcac acagagctca tgatctggtt tataaaggcg gcttcatgac cctcaattcc
     1201 atatagtatc actcccatca cagcatttcg atatcttcaa ccactttaac cttctccaga
     1261 ggatcatcat ctcaagccct tcataaatgt gagaccactc atcgcgacta gtagcggccg
     1321 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     1381 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1561 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     1621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1981 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     2341 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     2401 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     2461 caaattacgc cccgccctgc cactcgtcgc agtactgttg taattcatta agcattctgc
     2521 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2581 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2641 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgat acgaaaaaca
     2701 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2761 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agtgatgaaa
     2821 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2881 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     2941 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     3001 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     3061 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     3121 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     3181 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
     3241 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3301 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     3361 cggccgcttc tagagcgatg agtggtctcg
//
