LOCUS       sequence-226601-        2483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Terminator paf from P. chrysogenum domesticated into pUPD2 with 
            GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA
            assembly for A. tumefaciens-mediated genetic transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB030
FEATURES             Location/Qualifiers
     source          1..2483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      420..477
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(501..518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(672..1260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(752..771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1443..1537)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1558..2217)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2132..2151
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2379..2397
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2401..2444)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2447..2468
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gcttatggtg tctgcgatca ccagggcatt taatggtttt tggttccctt cttgttggtg
       61 atatgcgaga tgccctgtga ttctcgaagc ttactaccct acacccacaa ggactcgaac
      121 caaggactgc tcggtgggtg atacatatac acccagtatc tatccagctt caattttcgg
      181 cgaattttgt ttcttatttc ataaagacac tcgtttgata tctagctaga tattgttgct
      241 catcaacgaa atggttgtag attatcgaat atatccagca tcttttgatc gtagtcggaa
      301 gtgaaatgga gtactatgat acgacacatg tacattgtaa gcagaaatag gctagaggga
      361 taactatcaa actgctgccg cttgagacca ctcatcgcga ctagtagcgg ccgctgcagt
      421 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
      481 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      541 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      601 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      661 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      841 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1081 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     1441 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     1501 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     1561 cgccccgccc tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1621 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1681 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1741 gtttaaatca aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc
     1801 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1861 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc
     1921 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1981 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2041 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2101 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2161 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2221 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     2281 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     2341 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     2401 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     2461 ttctagagcg atgagtggtc tcg
//
