LOCUS       sequence-226624-        8946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PegA glycosyltansferase in streptomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Stephens N, Rawlings B, Caffrey P
  TITLE     Versatility of enzymes catalyzing late steps in polyene 67-121C 
            biosynthesis.
  JOURNAL   Biosci Biotechnol Biochem. 2013;77(4):880-3. doi: 
            10.1271/bbb.120961. Epub 2013 Apr 7.
  PUBMED    23563553
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2013"; volume: "77(4):880-3. doi"; 
            pages: " 10.1271/bbb.120961. Epub 2013 Apr 7"
COMMENT     Sequence Label: pIAGO-pegA1
FEATURES             Location/Qualifiers
     source          1..8946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(124..146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     246..265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     459..481
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     473..490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     474..490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2347..2363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2347..2363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2360..2382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2371..2387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2395..2401,2402..2419,2420..2425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2440..2461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7051..7068)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7222..7810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7302..7321)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7981..8772,8773..8841))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8604..8623
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8842..8946)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
      121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
      181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
      301 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
      361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      421 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      481 gacggccagt gaattcgagc tcggtaccag cccgacccga gcacgcgccg gcacgcctgg
      541 tcgatgtcgg accggagttc gaggtacgcg gcttgcaggt ccaggaaggg gacgtccatg
      601 cgagtgtccg ttcgagtggc ggcttgcgcc cgatgctagt cgcggttgat cggcgatcgc
      661 aggtgcacgc ggtcgatctt gacggctggc gagaggtgcg gggaggatct gaccgacgcg
      721 gtccacacgt ggcaccgcga tgctgttgtg ggcacaatcg tgccggttgg taggatctgc
      781 taaggaaatt cggcaccgat cggtcgcagg atcggtgcgg gtgtccttgc agggcaccgg
      841 caaacgcccg agcgtaggag acgagatgac atcgcatcgt cccatcctgt tctgttgcac
      901 ccactccacc ggacaggcca cgtccagcct ggttttggcc ggcgagttcg ccgcccgcgg
      961 cgtcaagaac ctgtggtttg cgtccgacga caaccaccgt gcggcggtcg ccgggctgag
     1021 cggggcgagc gaggtcggct tcgtctcgac gggtccggtc aatccccgtg tggcgccgac
     1081 catgtgggac gacgagacgt atcgcagcat cacccagcgg tcccggtgga aaggcaatcg
     1141 agcacggctg cgccagctca tggacccggt gcaccacgcc gaccggttcc gccagctgga
     1201 cgcggcggtg cagcggatcc agccagccct catggtgatc aacaacctct gtatccacgg
     1261 catccaggtg gcgatgaagc ggggtgtgcc gtatgtcatc accggatcgt cgctggccag
     1321 cgacatctgg cagttcgatc tgccggacga ctacccggtg ccgtactccg gcctgccccg
     1381 gcggatgaac ctgcggcagc ggatcggcaa tcggctcttc cgccaccgca acctgctgtc
     1441 catgctggat ccggcactgc tcaggacggt tggcgccatg ctgaaggcca ccgacgagct
     1501 gggcctggac cgccgccagc tgcagcagcg cgcctggatg gaggaggcgg agctggtgct
     1561 ctgcttctcg gtgttcggcc tggactatcc cttcccccac ccgcccaagt tgcagatggt
     1621 cggcgcgatg gtcccgccgc tgccgcagaa cccggtgcct ggcgagaccg accggtggct
     1681 ggatgcacat ccgtcggtgg tcttcatcgc cttcggcact atcacccggc tcaccaaggc
     1741 ggaggtgcgg gcggtggtcg acgtggcccg gcggctgggc gagcggcacc acgtgctgtg
     1801 gaagttgccc cgtgagcagc agcggttcct gcctcccgcc gcggagctgc ccgccaacct
     1861 tcgcgtggag gactggttgc cctctcaata cgacgtgctc gcccactcga acgtccgggt
     1921 gttcttcggc cacggcggca acaacagctt ccacgagggg atctacttcg gaaagccgtc
     1981 actagtgcgg cccctgtggt ttgactgcct ggaccacgcc gttcgggccg tggacagcgg
     2041 cgtagggctc agtgtcgcgc cgggcaccat ggacccggca gagatccaca gcaagctcac
     2101 cgcgcttctg gacgacacgt cgttccgcgc gcgggccgag cacttcgccc agattcagca
     2161 cgaggccggc ggggtacgcc gggccgccga cctcattctg cgctgcgcgg cggtgacgag
     2221 cgccggccag gacgagcagc cggcggtcca gcggtgatct gcagccgggt gccgccgctc
     2281 ggctgacggc gtcgtcggcg gcggccttcc gtccggctga cgtgccttaa gcttggcgta
     2341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agggaccggc
     2521 gccacaccgg cttgcgccgg ctcacggtct cggacggcgc gaagccttcg caagtacctt
     2581 cgtgactacc cggtggacgc gttctccatg ctcaagggga tgcgcaaggc gatggaactc
     2641 atcggctacc cgccgacgtc cacgagcgtt ttcacagtcg aggcgatcgc ggaaggcgtc
     2701 agcgccatcg agtcgttcaa gggcgtcggc cgcgccttcg aggagtgccc gcccggacca
     2761 cgaccgttga cctcgatatg tcagtggtga tcgctgccgt gtgaggtgat catcactgac
     2821 gaatcgaggt cgaggaaccg agcgtccgag gaacagaggc gcttatcggt tggccgcgag
     2881 attcctgtcg atcctctcgt gcagcgcgat tccgagggaa acggaaacgt tgagagactc
     2941 ggtctggctc atcatgggga tggaaaccga ggcggaagac gcctcctcga acaggtcgga
     3001 aggcccaccc ttttcgctgc cgaacagcaa ggccagccga tccggattgt ccccgagttc
     3061 cttcacggaa atgtcgccat ccgccttgag cgtcatcagc tgcataccgc tgtcccgaat
     3121 gaaggcgatg gcctcctcgc gaccggagag aacgacggga agggagaaga cgtaacctcg
     3181 gctggccctt tggagacgcc ggtccgcgat gctggtgatg tcactgtcga ccaggatgat
     3241 ccccgacgct ccgagcgcga gcgacgtgcg tactatcgcg ccgatgttcc cgacgatctt
     3301 caccccgtcg agaacgacga cgtccccacg ccggctcgcg atatcgccga acctggccgg
     3361 gcgagggacg cgggcgatgc cgaatgtctt ggccttccgc tcccccttga acaactggtt
     3421 gacgatcgag gagtcgatga ggcggaccgg tatgttctgc cgcccgcaca gatccagcaa
     3481 ctcagatgga aaaggactgc tgtcgctgcc gtagacctcg atgaactcca ccccggccgc
     3541 gatgctgtgc atgaggggct cgacgtcctc gatcaacgtt gtctttatgt tggatcgcga
     3601 cggcttggtg acatcgatga tccgctgcac cgcgggatcg gacggatttg cgatggtgtc
     3661 caactcagtc atggtcgtcc taccggctgc tgtgttcagt gacgcgattc ctggggtgtg
     3721 acaccctacg cgacgatggc ggatggctgc cctgaccggc aatcaccaac gcaaggggaa
     3781 gtcgtcgctc tctggcaaag ctccccgctc ttccccgtcc gggacccgcg cggtcgatcc
     3841 ccgcatatga agtattcgcc ttgatcagtc ccggtggacg cgccagcggc ccgccggagc
     3901 gacggactcc ccgacctcga tcgtgtcgcc ctgagcgtcc acgtagacgt tgcgtgagag
     3961 caggactggg ccgccgccga ccgcaccgcc ctcaccaccg accgcgaccg cgccatggcc
     4021 gccgccgacg gcctggtcgc cgccgccgcc cgccggttcg gcgcctgacc cgaccaaccc
     4081 ccgcgggccc ccacccacca ggagaccgac catgaccgac ttcgacggac gcctgaccga
     4141 ggggaccgtg aacctggtcc aggaccccaa cggcggtggc tggtccgccc actgcgctga
     4201 gcccggttgc gactgggccg acttcgccgg accgctcggc ttccagggcc tcgtggccat
     4261 cgctcgccga cacacgcact gaccgcacgt caaagccccg ccggatcacc ggcggggctc
     4321 tcttcggccc tccaagtcac accagcccca aggggcgtcg ggagtggcgg agggaacctc
     4381 tggcccgatt ggtgccagga ttcccaccag accaaagagc aacgggccgg acttcgcacc
     4441 tccgacccgt ccgctcccag actcgcgccc cttagccggg cgagacagga acgttgctcg
     4501 tgcccagagt acggagcgat gccgaggcat tgccagatcg gcccgccggg ccccgctgcc
     4561 actgcgggac cgcaattgcc cacacaccgg gcaaacggcc gcgtatctac tgctcagacc
     4621 gctgccggat ggcagcgaag cgggcgatcg cgcgtgtgac gcgagatgcc gcccgaggca
     4681 aaagcgaaca ccttgggaaa gaaacaacag agtttcccgc acccctccga cctgcggttt
     4741 ctccggacgg ggtggatggg gagagcccga gaggcgacag cctctcggaa gtaggaagca
     4801 cgtcgcggag cgacgctgcc cgactgcgga aagccgcccg gtacagccgc cgccggacgc
     4861 tgtggcggat cagcggggac gccgcgtgca agggctgcgg ccgcgccctg atggaccctg
     4921 cctccggcgt gatcgtcgcc cagacggcgg ccggaacgtc cgtggtcctg ggcctgatgc
     4981 ggtgcgggcg gatctggctc tgcccggtct gcgccgccac gatccggcac aagcgggccg
     5041 aggagatcac cgccgccgtg gtcgagtgga tcaagcgcgg ggggaccgcc tacctggtca
     5101 ccttcacggc ccgccatggg cacacggacc ggctcgcgga cctcatggac gccctccagg
     5161 gcacccggaa gacgccggac agcccccggc ggccgggcgc ctaccagcga ctgatcacgg
     5221 gcggcacgtg ggccggacgc cgggccaagg acgggcaccg ggccgccgac cgcgagggca
     5281 tccgagaccg gatcgggtac gtcggcatga tccgcgcgac cgaagtcacc gtggggcaga
     5341 tcaacggctg gcacccgcac atccacgcga tcgtcctggt cggcggccgg accgaggggg
     5401 agcggtccgc gaagcagatc gtcgccacct tcgagccgac cggcgccgcg ctcgacgagt
     5461 ggcaggggca ctggcggtcc gtgtggaccg ccgccctgcg caaggtcaac cccgccttca
     5521 cgcccgacga ccggcacggc gtcgacttca agcggctgga gaccgagcgc gacgccaacg
     5581 acctcgccga gtacatcgcc aagacccagg acgggaaggc gcccgccctc gaactcgccc
     5641 gcgccgacct caagacggcg accggcggga acgtcgcccc gttcgaactc ctcggacgga
     5701 tcggggacct gaccggcggc atgaccgagg acgacgccgc cggggtcggc tcgctggagt
     5761 ggaacctctc gcgctggcac gagtacgagc gggcaacccg gggacgccgg gccatcgaat
     5821 ggacccgcta cctgcggcag atgctcgggc tcgacggcgg cgacaccgag gccgacgacc
     5881 tcgatctgct cctggcggcc gacgccgacg gcggggagct gcgggccggg gtcgccgtga
     5941 ccgaggacgg atggcacgcg gtcacccgcc gcgccctcga cctcgaggcg acccgggccg
     6001 ccgaaggcaa ggacggcaac gaggattcgg cggccgtggg cgaacgggtg cgggaggtcc
     6061 tggcgctggc cgacgcggcc gacacagtgg tggtgctcac ggcgggggag gtggccgagg
     6121 cgtacgccga catgctcgcc gccctcgccc agcgccgcga ggaagcaact gcacgccgac
     6181 ggcgagagca ggacgacgac caggacgacg acgccgacga ccgccaggag cgggccgccc
     6241 ggcacatcgc ccggctcgca agtgggccca cttcgcacta actcgctccc ccccgccgta
     6301 cgtcatcccg gtgacgtacg gcgggggtcg gtgacgtacg cggcgacggc ggccggggtc
     6361 gaagccgcgg gagtaatcct gggattactc gcccggggtc ggccccgccg gcacttcgtg
     6421 caggcggtac ctcgcgcccg actcgcctcg ctacgagacg tgccgcgtac ggtcgtcggc
     6481 catgagcacc accaccccca gggacgccga cggcgcgaag ctctgcgcct ggtgcggctc
     6541 ggagatcaag caatccggcg tcggccggag ccgggactac tgccgccgct cctgccgcca
     6601 gcgggcgtac gaggcccggc gccagcgcga ggcgatcgtg tccgccgtgg cgtcggcagt
     6661 cgctcgccga gatacgtcac gtgacgaaat gcagcagcct tccattccgt cacgtgacga
     6721 aactcgggcc gcaggtcaga gcacggttcc gcccgctccg tcgtggccgt agtacgagac
     6781 gtcggtgacc gttgccctga ccgcgccgac ggagtcctcg ccggtgaggc ggagttgttc
     6841 cggacggagc aggaccgtgc cggcgcgcag gccgccgggc ggagcggcga ggggcaccgt
     6901 gcccaaggcg gtccgggccg tgtcgccgga gtcgacgtcg acggtgccgg ggagcaggac
     6961 ggcgtcgccg acgaagccgg cgaccctgca ttaatgaatc ggccaacgcg cggggagagg
     7021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7201 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7621 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7741 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7801 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7861 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7981 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8041 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8101 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8161 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8221 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8281 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8341 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8401 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8461 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8521 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8581 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8641 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8701 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8761 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8821 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8881 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8941 tccgcg
//
